Phosphate solubilization potential and stress tolerance of rhizobacteria from rice soil in Northern Thailand.
01 Feb 2009-World Journal of Microbiology & Biotechnology (Springer Netherlands)-Vol. 25, Iss: 2, pp 305-314
TL;DR: The isolates could exhibit more than two or three plant growth-promoting (PGP) traits, which may promote plant growth directly or indirectly or synergistically, as evidenced by phenotypic biochemical test and quantitative assay using spectrophotometry.
Abstract: Plant growth-promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. A total of 216 phosphate-solubilizing bacterial isolates were isolated from different rice rhizospheric soil in Northern Thailand. These isolate were screened in vitro for their plant growth-promoting activities such as solubilization of inorganic phosphate, ammonia (NH3), catalase and cell wall-degrading enzyme activity. It was found that 100% solubilized inorganic phosphate, 77.77% produced NH3 and most of the isolates were positive for catalase. In addition, some strains also produced cell wall-degrading enzymes such as protease (7%), chitinase (1%), cellulase (3%) and β-glucanase (3%), as evidenced by phenotypic biochemical test and quantitative assay using spectrophotometry. The isolates could exhibit more than two or three plant growth-promoting (PGP) traits, which may promote plant growth directly or indirectly or synergistically. Part of this study focused on the effect of NaCl, temperature, and pH on a specific the bacterial isolate Acinetobacter CR 1.8. Strain CR 1.8 was able to grow on up to 25% NaCl, between 25 and 55°C, and at pH 5–9. Maximum solubilization of tricalcium phosphate and aluminium phosphate was obtained at neutral pH, and 37°C. Strain CR 1.8 had protease activity but no cellulase, β-glucanase and cellulase activities.
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TL;DR: Two stress tolerant phosphate solubilizing rhizobacteria Arthrobacter sp.
Abstract: The importance of rhizospheric microbial phosphate solubilization has now been well documented. However, the performance of these microbes is greatly affected by various environmental stresses such as salt stress, pH stress, temperature stress etc. In this study, two stress tolerant phosphate solubilizing rhizobacteria Arthrobacter sp. and Bacillus sp. have been isolated from tomato rhizosphere and characterized with various morphological and biochemical tests. Phosphate solubilizing bacteria were screened on the basis of their phosphate solubilization and strains with high phosphate solubilizing ability were then tested against wide range of temperature, pH, and salt stresses. Their ability to solubilize other insoluble phosphates, such as ferric phosphate (FePO4) and aluminum phosphate (AlPO4) was also studied. In addition to phosphate solubilizing ability these strains also demonstrated various plant growth promoting and biocontrol activities including indole acetic acid (IAA) production. These two strains have the potential to be used as plant growth promoting rhizobacteria (PGPR).
149 citations
Cites background from "Phosphate solubilization potential ..."
...Phosphate solubilizing microbes have been routinely isolated from rhizospheric soil of various plants such as rice (Chaiharn and Lumyong, 2009), wheat (Ahmad et al., 2008), soybean (Son et al., 2006), mustard (Chandra et al., 2007), aubergine (Ponmurugan and Gopi, 2006), and chili (Ponmurugan and…...
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TL;DR: In this context, isolation, identification and characterization of soil PSMs are considered to be effective in broadening the spectrum of phosphate solubilizers available for field application.
Abstract: Phosphate solubilizing microorganisms (PSMs) offer an ecologically acceptable mean for converting insoluble phosphate to soluble forms making them available for plants to absorb. Several bacterial strains (Pseudomonas, Bacillus, Rhizobium and Enterobacter) and fungal strains (Aspergillus and Penicillium) have so far been recognized as powerful phosphate solubilizers. Insoluble phosphates are converted into available forms by phosphate solubilizing microorganisms via the process of acidification, chelation, exchange reactions and production of organic acid. Though phosphorus is found to be a limiting factor in many soils, application of PSMs as biofertilizers or bioconverters for solubilizing fixed phosphorus has not yet been successfully practiced. In this context, isolation, identification and characterization of soil PSMs are considered to be effective in broadening the spectrum of phosphate solubilizers available for field application.
Key words: Phosphate solubilization, insoluble phosphate, organic acid, Phosphate solubilizing microorganisms (PSMs).
123 citations
Cites background from "Phosphate solubilization potential ..."
...PSMs have been isolated from soil of various plants such as walnut (Xuan Yu, 2011), rice (Chaiharn and Lumyong, 2009), mustard (Chandra et al., 2007), oil palm (Fankem et al., 2006), soybean (Son et al., 2006), aubergine and chili (Ponmurugan and Gopi, 2006), and maize (Alam et al., 2002)....
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TL;DR: The results confirm promising applications of the endophytic bacterial isolates obtained in the study and also their possible growth promoting effect in P. nigrum.
Abstract: Piper nigrum is an interesting plant to study the endophytic microbial factors affecting plant growth because of its unique features. Endophytic bacterial isolation from the plant resulted in the isolation of twelve bacterial isolates which were screened for various plant growth promoting properties like phosphate solubilization, ACC deaminase production, siderophore production etc. Interestingly, seven isolates were found to have IAA biosynthetic potential. Bacterial isolates with multiple plant growth promoting properties were studied for their growth promoting effect on Vigna radiata seedlings. This resulted in the identification of Klebsiella sp. (PnB 10) and Enterobacter sp. (PnB 11) as the isolates with excellent growth promoting properties. The results confirm promising applications of the endophytic bacterial isolates obtained in the study and also their possible growth promoting effect in P. nigrum.
123 citations
TL;DR: The SMS biofertilizer produced by P. farinose significantly improved the growth of soybean in pot experiments, demonstrating a tremendous potential in agricultural application.
85 citations
Cites background from "Phosphate solubilization potential ..."
...Mathurot and Saisamorn (2009) reported a bacterial isolate Acinetobacter CR 1.8 was able to grow on up to 25% NaCl....
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01 Jan 2012
TL;DR: In this paper, a review of the status of salinization and constraints related with it, and future research strategies for the development of better inoculants in saline-affected regions, particularly in context to the developing countries, is presented.
Abstract: The economy of many countries relies on agriculture. Salinity is one of the major constraints that limit crop productivity, particularly in arid and semiarid regions. The development of salt-tolerant crops is not an easy and economical approach for sustainable agriculture, whereas microbial inoculation to alleviate salt tolerance is a better option because it minimizes production costs and environmental hazards. The aim of the present review is to point out the status of salinization and constraints related with it, and to draw the focus on future research strategies for the development of better inoculants in saline-affected regions, particularly in context to the developing countries.
81 citations
Cites background from "Phosphate solubilization potential ..."
...fertilizer by mobilizing the fertilizer constituents present in the soil and at the same time reducing costs and improving crop yields (Chaiharn and Lumyong 2009)....
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TL;DR: Genetic manipulation of phosphate-solubilizing bacteria to improve their ability to improve plant growth may include cloning genes involved in both mineral and organic phosphate solubilization, followed by their expression in selected rhizobacterial strains.
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"Phosphate solubilization potential ..." refers background in this paper
...Phosphate in soil mostly exists in insoluble (bound) forms and the concentration of soluble phosphate in soil solution is very low (400–1,200 mg kg of soil) (Rodriguez and Fraga 1999)....
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