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Journal ArticleDOI

Phospholipids of Trichophyton rubrum.

01 Nov 1974-Medical Mycology (Sabouraudia)-Vol. 12, Iss: 3, pp 281-286
TL;DR: Trichophyton rubrum grown in Sabouraud's liquid medium contains phosphatidyl inositol, polyphosphatids inositols, and the relative proportion of these components in total phospholipid fraction remains almost constant throughout the culture life of this fungus.
Abstract: Trichophyton rubrum grown in Sabouraud's liquid medium contains phosphatidyl inositol, polyphosphatidyl inositol, phosphatidyl choline, phosphatidyl serine, phosphatidyl ethanolamine phosphatidyl glycerol and phosphatidic acid in the polar lipid fraction of its mycelia. The relative proportion of these components in total phospholipid fraction remains almost constant throughout the culture life of this fungus.
Citations
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Journal ArticleDOI
TL;DR: 3-O-acyl-quercetines, being more active and more lipophilic, could be more effective than Q when applied to the skin or mucosae, and deserve to be studied further.

94 citations

Journal ArticleDOI
01 Dec 1997-Mycoses
TL;DR: A total of 123 isolates of 14 species of dermatophytes and yeasts were screened for the activity of five extracellular enzymes including elastase, keratinase, protease, lipase and phospholipase, by using solid media.
Abstract: A total of 123 isolates of 14 species of dermatophytes and yeasts were screened for the activity of five extracellular enzymes including elastase, keratinase, protease (gelatinase), lipase and phospholipase, by using solid media. The optimal production and activity of each enzyme was determined with regard to different pH and temperatures. Keratinase activity was high with all the tested fungi with exception of Malassezia furfur. Protease (gelatinase) was produced only by dermatophytes. Elastase was secreted by three dermatophytes viz. Microsporum gypseum, Trichophyton mentagrophytes var. mentagrophytes and T. verrucosum, whereas lipase and phospholipase were detected in all the species except T. violaceum.

91 citations

Journal ArticleDOI
TL;DR: An exocellular proteinase produced by Trichophyton rubrum in a glucose-peptone broth was purified from lyophilized and dialysed culture filtrate of the dermatophyte and showed an alkaline pH optimum and was not activated by divalent metal ions but inhibited strongly by phenylmethylsulfonylfluoride.
Abstract: An exocellular proteinase produced by Trichophyton rubrum in a glucose-peptone broth was purified from lyophilized and dialysed culture filtrate of the dermatophyte by Sephadex G-100 gel filtration and preparative polyacrylamide gel electrophoresis. The purified enzyme was a homogeneous protein of molecular weight 34 700 and it could hydrolyse azoalbumin, casein, bovine serum albumin, α-N-benzoyl-L-arginine ethyl ester and p-toluenesulfonyl-L-arginine methyl ester but not N-benzoyl-L-tyrosine ethyl ester, α-N-benzoyl-DL-arginine-p-nitroanilide and keratin. The enzyme showed an alkaline pH optimum and was not activated by divalent metal ions but inhibited strongly by phenylmethylsulfonylfluoride. Thus the enzyme was identified as an alkaline serine proteinase.

70 citations

Book ChapterDOI
TL;DR: F fungi are an ideal system for the productive correlation of structural and biochemical aspects of lipid physiology in growth and differentiation, and the chapter discusses lipids and fungal membranes.
Abstract: Publisher Summary The physiology of fungal lipids can be defined as the relation of lipids to the process of life in fungi. Besides the essential presence of lipids as membrane components of the endoplasmic reticulum, plasmalemma and organelles, a variety of lipid-containing inclusions occur in plant cells, usually becoming most prominent in reproductive organs and spores. Lipids are also found as cell-wall components and on the surfaces of spores. Lipid inclusions are often abundant within vegetative mycelium and their ultrastructural distribution within vegetative hyphae has received less attention than that in reproductive organs. Reproductive structures show some characteristic patterns of lipid distribution in different taxonomic groups of fungi. Aspects of morphological organization and life cycle used in fungal taxonomy correspond very satisfactorily with wall chemistry. The relative ease of identification of lipid structures and the convenient manipulation of physical and nutrient growth conditions permitted by their hyphal organization, make fungi an ideal system for the productive correlation of structural and biochemical aspects of lipid physiology in growth and differentiation. The chapter discusses lipids and fungal membranes. The primary functions of membranes are to provide a permeability barrier and sites for active transport of ions and facilitated diffusion of hydrophilic molecules. Membranes consist mostly of lipid, protein, and some water. The lipid is a mixture in which phosphoglycerides usually predominate.

67 citations

References
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Journal ArticleDOI
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.

59,550 citations

Journal ArticleDOI
TL;DR: Information obtained from this study and from previous investigations of the properties of silicic acid were used to elaborate a standardized method for the separation of lipide groups based on adsorption chromatography with silicIC acid.

815 citations

Book ChapterDOI
01 Jan 1965
TL;DR: The authors showed that free amino acids and peptides are markedly hydrophilic compounds, only slightly soluble in nonaqueous solvents, and this must be borne in mind when sampling and preparing materials for TLC as well as when choosing the solvent.
Abstract: Free amino acids and peptides are markedly hydrophilic compounds, only slightly soluble in non-aqueous solvents. This must be borne in mind when sampling and preparing materials for TLC as well as when choosing the solvent. Some data on solubilities of a few amino acids in various solvents which illustrate this are:

143 citations

Journal ArticleDOI
TL;DR: Oil ether extracts of 14 species of the dermatophytes, representing one group of closely related filamentous fungi, were examined for the presence of free sterols in continuation of investigations on the chemical constituents of pathogenic fungi.

28 citations