Abstract: Energy is considered a conserved quantity. With numerous applications, it's available in a different form from various resources. Hence production energy depends on the available resources and efficiency. The purpose of the research work is to produce bioethanol from waste rose flower petals. Four different steps namely pre-treatment, hydrolysis fermentation, and distillation were used for bioethanol conversion. Advanced techniques such as Fourier infrared transformation, scanning electron micrography, X-ray diffraction, and thermal analysis were employed to determine the best precondition. At a most favorable condition of hydrolysis time = 120 h; temperature 30 °C, enzyme concentration = 1.75 g/L, pH 5.5, and mixing speed 120 rpm give the highest glucose yields 40.8 g/L with commercial enzyme Accellerase 1500. In addition to that at pH 4.5, fermentation time 192hrs and temperature 30 °C, a maximum of 29.5 g/L bioethanol was produced. The bioethanol was confirmed by 1HNMR, gas chromatography analysis, and infrared transformation.
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