Journal ArticleDOI
Pilot-scale culture of adventitious roots of ginseng in a bioreactor system
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TLDR
Ginsengnoside profiles of these multiple adventitious roots were similar to profiles of field-grown ginseng roots when analyzed by HPLC, and saponin content obtained from small-scale and pilot-scale balloon type bubble bioreactors was around 1% based on dry weight.Abstract:
A pilot-scale culture of multiple adventitious roots of ginseng was established using a balloon-type bubble bioreactor. Adventitious roots (2 cm) induced from callus were cultured in plastic Petri dishes having 20 ml of solid Schenk and Hildebrandt (1972) medium containing 3% sucrose, 0.15% gelrite, and 24.6 μM indole-3-butric acid. An average of 29 secondary multiple adventitious roots were produced after 4 weeks of culture. These secondary roots were elongated on the same medium, reaching a length of 5 cm after 6 weeks of culture. A time course study revealed that maximum yields in 5-l and 20-l bioreactors were approximately 500 g and 2.2 kg at day 42 with 60 g and 240 g inoculations, respectively. Cutting twice during the culture increased the total amount of biomass produced. The root biomass in a 20-l balloon-type bubble bioreactor was 2.8 kg at harvest with 240 g of inoculum after 8 weeks of culture. The total saponin content obtained from small-scale and pilot-scale balloon type bubble bioreactors was around 1% based on dry weight. Inoculation of 500 g fresh weight of multiple adventitious roots into a 500 l balloon-type bubble bioreactor with cutting at 4 and 6 weeks after inoculation produced approximately 74.8 kg of multiple roots. The ginsengnoside profiles of these multiple adventitious roots were similar to profiles of field-grown ginseng roots when analyzed by HPLC.read more
Citations
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Journal ArticleDOI
Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation
TL;DR: By following stage-specific strategies, it is possible to produce large amounts of biomass with an increase in the accumulation of secondary compounds, which are used as pharmaceuticals, agrochemicals, flavors, fragrances, coloring agents, biopesticides, and food additives.
Journal ArticleDOI
Application of bioreactor systems for large scale production of horticultural and medicinal plants
TL;DR: Low cost and less labour-intensive clonal propagation through the use of modified air-lift, bubble column, bioreactors, together with temporary immersion systems for the propagation of shoots, bud-clusters and somatic embryos is described.
Journal ArticleDOI
Dammarenediol-II synthase, the first dedicated enzyme for ginsenoside biosynthesis, in Panax ginseng
TL;DR: In this paper, a new OSC gene (named as PNA) was expressed in a lanosterol synthase deficient (erg7) Saccharomyces cerevisiae strain GIL77.
Journal ArticleDOI
Secondary metabolism of pharmaceuticals in the plant in vitro cultures: strategies, approaches, and limitations to achieving higher yield
Tasiu Isah,Shahid Umar,Abdul Mujib,Maheshwar Prasad Sharma,P. E. Rajasekharan,Nadia Zafar,Arajmand Frukh +6 more
TL;DR: The effort has shifted towards optimizing culture conditions for high-value pharmaceuticals production through the application of cell line selection, elicitation, precursor feeding, two-phase co-culture among cell, tissue, and organ culture approaches.
Journal ArticleDOI
Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor
TL;DR: The results of this study contribute to optimization and development of bioreactor technology for adventitious root cultures of H. perforatum for the production of hypericin.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures
Roy U. Schenk,A. C. Hildebrandt +1 more
TL;DR: The composition of a medium is described that proved useful to culture callus of a variety of monocotyledonous and dicotylingonous plants and facilitated work with single cells and the enzymatic removal of cell walls in related studies.
Journal ArticleDOI
Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes.
TL;DR: Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes, and synthesized the same saponins, ginsenosides, as those of the native root.
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