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Journal ArticleDOI

Plant regeneration via embryo and shoot bud formation from flower-stalk explants of Oncidium Sweet Sugar

01 Aug 2000-Plant Cell Tissue and Organ Culture (Kluwer Academic Publishers)-Vol. 62, Iss: 2, pp 95-100
TL;DR: Segments taken from flower-stalk internodes of Oncidium Sweet Sugar formed somatic embryos and shoot buds directly from wound surfaces or via nodular masses proliferation within 1.5 months, when cultured on a Gelrite-gelled 1/2-MS basal medium supplemented with thidiazuron in darkness.
Abstract: Segments taken from flower-stalk internodes of Oncidium Sweet Sugar formed somatic embryos and shoot buds directly from wound surfaces or via nodular masses proliferation within 1.5 months, when cultured on a Gelrite-gelled 1/2-MS basal medium supplemented with thidiazuron (0.1–3 mg l−1) in darkness. In light, when subcultured, these nodular masses proliferated into green compact callus, and produced somatic embryos, shoot buds and/or yellowish abnormal structures spontaneously. Supplementing 0.1–1 mg l−1 NAA enhanced embryo formation, but retarded proliferation of shoot buds and yellowish abnormal structures. Somatic embryos that directly formed from wound surfaces of flower stalk explants usually developed into abnormal structures, but the callus-derived embryos could germinate into PLBs and eventually developed to normal plantlets on a hormone-free basal medium for 3–4 weeks. Both the embryo-and shoot bud-derived regenerants developed into healthly plantlets when potted in sphagnum moss and acclimatized in the greenhouse.
Citations
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Journal ArticleDOI
TL;DR: This work endeavours to include the major investigations on explant-based orchid tissue culture starting from the pioneering works of Rotor and Wimber to date to date.

263 citations

Journal ArticleDOI
TL;DR: The red light spectrum enhanced induction, proliferation, and the carbohydrate contents of PLBs, as well as subsequent plantlet lengths, while the blue spectrum promoted differentiation, protein accumulation, and enzyme activities in PLBs and developing plantlets.
Abstract: The effects of different spectral light distribution on in vitro induction and proliferation of Oncidium protocorm-like bodies (PLBs) and subsequent growth of plantlets were investigated. Shoot tips (5 mm in length) of proliferating shoots of Oncidium “Gower Ramsey” were vertically incubated on 1/2 Murashige and Skoog (MS) medium supplemented with 1.0 mg l−1 6-benzyladenine (BA), and grown under either monochromatic red light-emitting diodes (LEDs) (RR), blue LEDs (BB), yellow LEDs (YY) or green LEDs (GG). Cultures grown under fluorescent lamps (FL) were used as control. Selected FL-induced PLBs were cut into 3- to 4-mm sections and incubated on MS medium supplemented with 1.0 mg l−1 BA and 0.5 mg l−1 α-naphthaleneacetic acid (NAA), and grown under RR, BB, YY, GG, or FL. Moreover, FL-differented shoots (15 mm in length with two leaves) were incubated on 1/2 MS medium with 0.5 mg l−1 NAA, and grown under either FL, RR, 10% blue + 90% red LEDs (1BR), 20% blue + 80% red LEDs (2BR), 30% blue + 70% red LEDs (3BR), BB, 80% red + 10% blue + 10% far-red LEDs (RBFr), or 80% red + 10% blue + 10% green LEDs (RBG). Overall, the red light spectrum enhanced induction, proliferation, and the carbohydrate contents of PLBs, as well as subsequent plantlet lengths, while the blue spectrum promoted differentiation, protein accumulation, and enzyme activities in PLBs, as well as pigment content accumulation in PLBs and developing plantlets. The combination of red and blue LEDs resulted in higher energy efficiency as well as dry weight and enzyme activities in these plantlets.

122 citations


Cites methods from "Plant regeneration via embryo and s..."

  • ...Plant regeneration of Oncidium from somatic embryogenesis has been established by using flower stalk internodes ( Chen and Chang 2000a ), leaves, roots and stems (Chen and Chang 2000b )a s explants....

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  • ...Plant regeneration of Oncidium from somatic embryogenesis has been established by using flower stalk internodes (Chen and Chang 2000a), leaves, roots and stems (Chen and Chang 2000b) as explants....

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Journal ArticleDOI
TL;DR: In vitro propagation protocol for Dendrobium hybrids Sonia 17 and 28, two highly priced commercial cut flower cultivars through direct organogenesis from in vitro derived foliar explants was established by subsequent induction of protocorm-like bodies (PLBs) and its conversion to shoots.

120 citations

Journal ArticleDOI
TL;DR: An informative and broad overview of orchid biotechnology, addressing several important aspects such as molecular systematics, modern breeding, in vitro morphogenesis, protoplast culture, flowering control, flower color, somaclonal variation, orchid mycorrhiza, pathogen resistance, virus diagnosis and production of virus-free plants.
Abstract: This review provides an informative and broad overview of orchid biotechnology, addressing several important aspects such as molecular systematics, modern breeding, in vitro morphogenesis, protoplast culture, flowering control, flower color, somaclonal variation, orchid mycorrhiza, pathogen resistance, virus diagnosis and production of virus-free plants, functional genomics, genetic transformation, conservation biotechnology and pharmaceutical biotechnology. This resource will provide valuable insight to researchers who are involved in orchid biology and floriculture, using biotechnology to advance research objectives. Producing an improved orchid through biotechnology for industrial purposes or to serve as a model plant for pure and applied sciences is well within reach and many of the current techniques and systems are already employed at the commercial production level.

115 citations

Journal ArticleDOI
TL;DR: This review addresses the morphological, physiological, and cytogenetic abnormalities associated with the use of TDZ in vitro, and provides a summary of these abnormalities in several plant species.
Abstract: Thidiazuron (TDZ) is a proven effective and potent synthetic plant growth regulator for organogenic, regeneration, and developmental pathways, including axillary and adventitious shoot proliferation, somatic embryogenesis, and in vitro flowering. TDZ has facilitated the establishment of in vitro cultures for several plant species, especially woody and recalcitrant plants, which has enabled their genetic transformation and improvement. Despite the effectiveness and advantages of using TDZ, several drawbacks are associated with its application in plant tissue culture. This review addresses the morphological, physiological, and cytogenetic abnormalities associated with the use of TDZ in vitro, and provides a summary of these abnormalities in several plant species.

90 citations

References
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Journal ArticleDOI
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Abstract: In experiments with tobacco tissue cultured on White's modified medium (basal meditmi hi Tnhles 1 and 2) supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Subse(iueutly it was found Ihiit this jnoniotiou oi' f^rowih was due mainly though nol entirely to inorj^auic rather than organic con.stitttenls in the extract. In the isolation of Rrowth factors from plant tissues and other sources inorj '̂anic salts are fre(|uently carried along with fhe organic fraclioits. When tissue cultures are used for bioassays, therefore, il is necessary lo lake into account increases in growth which may result from nutrient elements or other known constituents of the medium which may he present in the te.st materials. To minimize interference trom rontaminaitis of this type, an altempt has heen made to de\\eh)p a nieditmi with such adequate supplies of all re(iuired tnineral nutrients and cotntnott orgattic cottslitueitls that no apprecial»le change in growth rate or yield will result from the inlroduclion of additional amounts in the range ordinarily expected to be present in tnaterials to be assayed. As a point of referetice for this work some of the culture media in mc)st common current use will he cotisidered briefly. For ease of comparis4)n Iheir mineral compositions are listed in Tables 1 and 2. White's nutrient .solution, designed originally for excised root cultures, was based on Uspeuski and Uspetiskaia's medium for algae and Trelease and Trelease's micronutrieni solution. This medium also was employed successfully in the original cttltivation of callus from the tobacco Iiybrid Nicotiana gtauca x A', tanijadorffii, atitl as further modified by White in 194̂ ^ and by others it has been used for the

63,098 citations

Journal ArticleDOI

22,988 citations

01 Jan 1962

16,251 citations


"Plant regeneration via embryo and s..." refers background in this paper

  • ...The basal medium contained 1/2-strength macro- and micro-elements of Murashige and Skoog (Murashige and Skoog, 1962) supplemented with (mg l−1): myo-inositol (100), niacin (0.5), pyridoxine HCl (0.5), thiamine HCl (0.1), glycine (2.0), peptone (1000), NaH2PO4 (170), sucrose (20,000), and Gelrite…...

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  • ...Abbreviations:MS – Murashige and Skoog basal medium; NAA – naphthaleneacetic acid; PLB – protocorm-like body; TDZ – 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea; 2,4-...

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  • ...The basal medium contained 1/2-strength macro- and micro-elements of Murashige and Skoog (Murashige and Skoog, 1962) supplemented with (mg l−1): myo-inositol (100), niacin (0.5), pyridoxine HCl (0.5), thiamine HCl (0.1), glycine (2.0), peptone (1000), NaH2PO4 (170), sucrose (20,000), and Gelrite (2,200)....

    [...]

  • ...The basal medium contained 1/2-strength macro- and micro-elements of Murashige and Skoog (Murashige and Skoog, 1962) supplemented with (mg l−1): myo-inositol (100), niacin (0....

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Journal ArticleDOI
TL;DR: The cytokinin activity of Thidiazuron was similar to that of the highly active N -phenyl- N ′-4-pyridylurea derivatives and to the most active cytokinins of the adenine type.

368 citations


"Plant regeneration via embryo and s..." refers background in this paper

  • ...TDZ, which has strong cytokinin activity (Mok et al., 1982) inducesin vitro morphogenesisin several orchid species (Ernst, 1994; Chen and Piluek, 1995; Nayak et al., 1997; Chang and Chang, 1998; Chen et al., 1999)....

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  • ...TDZ, which has strong cytokinin activity (Mok et al., 1982) inducesin vitro morphogenesisin several orchid species (Ernst, 1994; Chen and Piluek, 1995; Nayak et al....

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Journal ArticleDOI
TL;DR: Totipotent calli of Cymbidium ensifolium var.
Abstract: Totipotent calli of Cymbidium ensifolium var. misericors, a locally grown orchid of high commercial value, were induced from sections of pseudobulbs, rhizomes and roots of seed-derived plantlets on 1/2-strength Murashige and Skoog medium plus 10 mg/l 2,4-dichlorophenoxyacetic acid and 0.1 mg/l thiadiazuron. The calli could be maintained by subculturing in the same medium. The calli could be induced to develop further along one of three distinct morphogeneic routes: (1) production of rhizomes, (2) production of shoot buds, or (3) development of granular embryoids. Efficient mass propagation was possible via rhizome proliferation and embryoid formation.

134 citations


"Plant regeneration via embryo and s..." refers background in this paper

  • ...TDZ, which has strong cytokinin activity (Mok et al., 1982) inducesin vitro morphogenesisin several orchid species (Ernst, 1994; Chen and Piluek, 1995; Nayak et al., 1997; Chang and Chang, 1998; Chen et al., 1999)....

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