Polarization and depolarization metrics as optical markers in support to histopathology of ex vivo colon tissue
Deyan Ivanov,Viktor Dremin,Ekaterina Borisova,Alexander Bykov,Tatiana Novikova,Igor Meglinski,Razvigor Ossikovski +6 more
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TLDR
It was found that the Stokes-Mueller matrix polarimetry, combined with the appropriate filtering, decomposition algorithms and polarization/depolarization metrics calculations provides relevant optical markers of the colon tissue pathological conditions (healthy versus cancer), as confirmed by histopathology analysis.Abstract:
Tissue polarimetry holds great promise to improve the effectiveness of conventional cancer diagnostics and staging, being a fast, minimally invasive, and low-cost optical technique. We introduce an enhanced diagnostic method for ex vivo colon specimens assessment by utilizing Stokes and Mueller matrix polarimetry. The proposed method makes use of experimental Mueller matrices, measured from healthy and tumor zones of a colon specimen, as input data for post-processing algorithms that include physical realisability filtering, symmetric decomposition and estimation of various polarization and depolarization metrics for colon specimen diagnostics. We validated our results with the gold standard histological diagnostics provided by pathologists. It was found that the Stokes-Mueller matrix polarimetry, combined with the appropriate filtering, decomposition algorithms and polarization/depolarization metrics calculations provides relevant optical markers of the colon tissue pathological conditions (healthy versus cancer), as confirmed by histopathology analysis. This approach potentially provides physicians with valuable and complementary information that holds promises in helping with the diagnostics of colon tissue specimens.read more
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Polarization-Based Histopathology Classification of Ex Vivo Colon Samples Supported by Machine Learning
Deyan Ivanov,Viktor Dremin,Ts. Genova,Alexander Bykov,Tatiana Novikova,Razvigor Ossikovski,Igor Meglinski +6 more
TL;DR: The presented polarimetric model was able to support the physical interpretation of the results from previous studies and relate the latter to the samples’ health condition, respectively.
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Distinguishing tissue structures via polarization staining images based on different combinations of Mueller matrix polar decomposition parameters
Honghui He,Jan Hansen +1 more
TL;DR: In this article , an image fusion method based on color spaces was introduced to combine different Mueller matrix derived parameters to provide multi-dimensional structural information pixel by pixel in a single polarization staining image.
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Assessment of tissue pathology using optical polarimetry.
TL;DR: This article conducted a rigorous online survey to collect all published studies that report the two most common polarimetric variables (i.e., depolarization and retardance) for any type of tissue pathology.
Journal ArticleDOI
Automatic pseudo-coloring approaches to improve visual perception and contrast in polarimetric images of biological tissues
TL;DR: In this paper , two pseudo-colored methods are proposed based on the Euclidean distances of actual values of pixels and an average value taken over a given region of interest in the considered image.
References
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Depolarization and polarization indices of an optical system
José J. Gil,Eusebio Bernabeu +1 more
TL;DR: In this article, the authors derived the depolarization index and the direct and reciprocal polarization indices from the Mueller matrix of any optical system and used them to estimate the power of the optical system.
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Application of circularly polarized light for non-invasive diagnosis of cancerous tissues and turbid tissue-like scattering media
Britt Kunnen,Britt Kunnen,Callum M. Macdonald,Alexander Doronin,Steven L. Jacques,Michael R. Eccles,Igor Meglinski +6 more
TL;DR: It is demonstrated that the Stokes vector of backscattered light depicted on a Poincaré sphere can be used to assess a turbid tissue-like scattering medium and the utility of this approach for characterization of cancerous and non-cancerous tissue samples in vitro is investigated.