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Journal ArticleDOI

Poly(levodopa)-modified β-glucan as a candidate for wound dressings.

24 Jul 2021-Carbohydrate Polymers (Carbohydr Polym)-Vol. 272, pp 118485-118485
TL;DR: In this article, thermally polymerized curdlan hydrogel was modified via two different modes using levodopa instead of commonly used dopamine and then coupled with gentamicin - aminoglycoside antibiotic.
About: This article is published in Carbohydrate Polymers.The article was published on 2021-07-24 and is currently open access. It has received 11 citations till now. The article focuses on the topics: Curdlan & Self-healing hydrogels.
Citations
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Journal ArticleDOI
TL;DR: In this article , the authors proposed a new approach to prepare hydrogel dressings with low-cost, non-toxicity and good anti-bacterial performance in the treatment of infectious wounds.

4 citations

Journal ArticleDOI
TL;DR: In this paper , a sol-gel-based coating was prepared by utilizing the strong adhesion property of polydopamine (PDA) to provide a highly condensed solgel based coating ([email protected]) on Mg alloy AZ31.

3 citations

Journal ArticleDOI
01 Jan 2022
TL;DR: In this article , a poly(L-DOPA)-coated polycatecholamine layer was applied to polyester (PET) prostheses in reconstructive vascular surgery.
Abstract: Polyester (PET) prostheses are commonly used in reconstructive vascular surgery. The most serious complication after implantation is early or late infection of the graft. Therefore, there is high demand to protect prosthesis against bacterial adhesion and biofilm development. For this reason, in this work PET prostheses were first coated by highly adhesive polycatecholamine layer. The grafts were then coupled with gentamicin and studied in relation to morphological and structural properties, biological safety (contact with blood, reaction of vascular endothelial cells (HUVEC), Danio rerio fish), drug release and antibacterial activity. Among two tested catecholamine monomers, L-DOPA was found to be more effective precursor in this process than dopamine. For L-DOPA, assistance of Cu2+, Mg2+ and Na+ ions seems to increase the amount of further immobilized drug. Coated prostheses exhibited greater human endothelial cell proliferation increase and lower cytotoxic effect than uncoated. The modification reduced the hemolysis observed for pristine commercial graft and limited the rate of abnormalities in D. rerio larvae, confirming the safety of the proposed modification. The coating allowed to double the amount of immobilized antibiotic in comparison with uncoated graft which resulted in increased antibacterial activity and reduced bacterial adhesion against 4 bacterial strains prevalent in biomaterials infections. Overall, poly(L-DOPA)-coatings deposited on PET vascular grafts can effectively functionalize these prostheses for higher safety in biomedical applications.

3 citations

Journal ArticleDOI
TL;DR: In this article, a sol-gel-based coating was modified on AZ31 Mg alloy by levodopa (DOPA), and the longlasting corrosion protection mechanism was studied by multiple electrochemical testing methods and surface characterization techniques.
Abstract: Purpose This study aims to develop a sol-gel-based coating to provide a long-lasting corrosion protection on AZ31 Mg alloy. Silane-based sol-gel coatings have been successfully applied to Mg alloys for corrosion protection. However, the micro or nano defects formed during condensation and solidification will cause the coating failure during a long-lasting immersion in a saline solution. More durable corrosion-protective sol-gel coatings are needed. Design/methodology/approach A sol-gel-based coating was modified on AZ31 Mg alloy by levodopa (DOPA). The long-lasting corrosion protection mechanism was studied by multiple electrochemical testing methods and surface characterization techniques. Findings Long-term testing by electrochemical impedance spectroscopy in aqueous 0.1 M NaCl indicated that the modified DOPA@sol-gel coating exhibited significant corrosion protection performance (>14 days). In comparison, the DOPA-free sol-gel coating failed only after three days of testing. The improved corrosion protection is attributed to the self-polymerized DOPA filling to the micro or nano defects in the glassy cross-linked networks of the sol-gel coating, which greatly improves the compactness of the coating. Originality/value The method of this study is simple and easy to process, the raw materials are green and the protective effect is excellent, which is of significance for the study of magnesium alloy corrosion protection.

2 citations

Journal ArticleDOI
TL;DR: In this paper , aqueous resins are developed comprising bovine serum albumin (BSA) and poly(ethylene glycol diacrylate) (PEGDA) with engineered microbes for vat photopolymerization to create objects with a wide array of 3D form factors.
Abstract: Engineered living materials (ELMs) combine living cells with polymeric matrices to yield unique materials with programmable functions. While the cellular platform and the polymer network determine the material properties and applications, there are still gaps in the ability to seamlessly integrate the biotic (cellular) and abiotic (polymer) components into singular materials, then assemble them into devices and machines. Herein, the additive‐manufacturing of ELMs wherein bioproduction of metabolites from the encapsulated cells enhanced the properties of the surrounding matrix is demonstrated. First, aqueous resins are developed comprising bovine serum albumin (BSA) and poly(ethylene glycol diacrylate) (PEGDA) with engineered microbes for vat photopolymerization to create objects with a wide array of 3D form factors. The BSA‐PEGDA matrix afforded hydrogels that are mechanically stiff and tough for use in load‐bearing applications. Second, the continuous in situ production of l‐DOPA, naringenin, and betaxanthins from the engineered cells encapsulated within the BSA‐PEGDA matrix is demonstrated. These microbial metabolites bioaugmented the properties of the BSA‐PEGDA matrix by enhancing the stiffness (l‐DOPA) or resistance to enzymatic degradation (betaxanthin). Finally, the assembly of the 3D printed ELM components into mechanically functional bolts and gears to showcase the potential to create functional ELMs for synthetic living machines is demonstrated.

2 citations

References
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Journal ArticleDOI
19 Oct 2007-Science
TL;DR: Inspired by the composition of adhesive proteins in mussels, dopamine self-polymerization is used to form thin, surface-adherent polydopamine films onto a wide range of inorganic and organic materials, including noble metals, oxides, polymers, semiconductors, and ceramics.
Abstract: We report a method to form multifunctional polymer coatings through simple dip-coating of objects in an aqueous solution of dopamine. Inspired by the composition of adhesive proteins in mussels, we used dopamine self-polymerization to form thin, surface-adherent polydopamine films onto a wide range of inorganic and organic materials, including noble metals, oxides, polymers, semiconductors, and ceramics. Secondary reactions can be used to create a variety of ad-layers, including self-assembled monolayers through deposition of long-chain molecular building blocks, metal films by electroless metallization, and bioinert and bioactive surfaces via grafting of macromolecules.

8,669 citations

Journal ArticleDOI
TL;DR: It is emphasized that the facile synthesis of a GO membrane exploiting the ideal properties of inexpensive GO materials offers a myriad of opportunities to modify its physicochemical properties, potentially making the GO membrane a next-generation, cost-effective, and sustainable alternative to the long-existing thin-film composite polyamide membranes for water separation applications.
Abstract: We report a novel procedure to synthesize a new type of water separation membrane using graphene oxide (GO) nanosheets such that water can flow through the nanochannels between GO layers while unwanted solutes are rejected by size exclusion and charge effects. The GO membrane was made via layer-by-layer deposition of GO nanosheets, which were cross-linked by 1,3,5-benzenetricarbonyl trichloride, on a polydopamine-coated polysulfone support. The cross-linking not only provided the stacked GO nanosheets with the necessary stability to overcome their inherent dispensability in water environment but also fine-tuned the charges, functionality, and spacing of the GO nanosheets. We then tested the membranes synthesized with different numbers of GO layers to demonstrate their interesting water separation performance. It was found that the GO membrane flux ranged between 80 and 276 LMH/MPa, roughly 4–10 times higher than that of most commercial nanofiltration membranes. Although the GO membrane in the present deve...

1,224 citations

Journal ArticleDOI
TL;DR: Dopamine covalently modifies parkin in living dopaminergic cells, a process that increases parkin insolubility and inactivates its E3 ubiquitin ligase function, suggesting a mechanism for the progressive loss of parkin function in dopamine neurons during aging and sporadic Parkinson disease.
Abstract: Inherited mutations in PARK2, the gene encoding parkin, cause selective degeneration of catecholaminergic neurons in the substantia nigra and locus coeruleus of the brainstem, resulting in early-onset parkinsonism. But the role of parkin in common, sporadic forms of Parkinson disease remains unclear. Here we report that the neurotransmitter dopamine covalently modifies parkin in living dopaminergic cells, a process that increases parkin insolubility and inactivates its E3 ubiquitin ligase function. In the brains of individuals with sporadic Parkinson disease, we observed decreases in parkin solubility consistent with its functional inactivation. Using a new biochemical method, we detected catechol-modified parkin in the substantia nigra but not other regions of normal human brain. These findings show a vulnerability of parkin to modification by dopamine, the principal transmitter lost in Parkinson disease, suggesting a mechanism for the progressive loss of parkin function in dopaminergic neurons during aging and sporadic Parkinson disease.

677 citations

Journal ArticleDOI
01 Dec 2018-Heliyon
TL;DR: A brief overview of concepts of bacterial biofilm formation, current state-of-the-art therapeutic approaches for preventing and treating biofilms, and the prevalence of such infections on medical devices is reviewed.

623 citations