Journal ArticleDOI
Polychromatic staining of plant cell walls by toluidine blue O
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TLDR
The relationship between the polychromatic staining observed and the lignification of the walls is discussed and the effects of various common fixatives and the pH of the staining solution are evaluated.Abstract:
1.
The polychromatic staining of plant cell walls by toluidine blue O is described and illustrated.
2.
The effects of various common fixatives and the effects of the pH of the staining solution are evaluated.
3.
Simple and rapid procedures are described for preparing stained temporary mounts of fresh material, or permanent mounts of embedded and sectioned material.
4.
The relationship between the polychromatic staining observed and the lignification of the walls is discussed.read more
Citations
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Journal ArticleDOI
Plant microtechnique: some principles and new methods
Ned Feder,Terence P. O'Brien +1 more
TL;DR: A method of fixation in acrolein and embedding in glycol methacrylate polymer is described in detail and in a wide range of plant specimens prepared in this way, stained sections 1-3 microns thick showed excellent preservation of tissue and cell structures.
Journal ArticleDOI
Influence of the Testa on Seed Dormancy, Germination, and Longevity in Arabidopsis
TL;DR: Both structural and pigmentation mutants deteriorated faster than the wild types during natural aging at room temperature, with structural mutants being the most strongly affected.
Journal ArticleDOI
Simple method for differential staining of paraffin embedded plant material using toluidine blue O
TL;DR: Differential staining of paraffin embedded plant material is accomplished without removing theparaffin, and lignified tissue, suberized tissue, and some tannins are stained blue-green, while nonlignified walls are stained red-purple.
Journal ArticleDOI
The Use of an Optical Brightener in the Study of Plant Structure
J. Hughes,Margaret E. McCully +1 more
TL;DR: An optical brightener Calcoflour White M2R New has been used to stain cell walls of higher plants and can be used either as a vital stain for intact plants or for hand sections and plastic-embedded thin sections.
Journal ArticleDOI
H2O2 plays different roles in determining penetration failure in three diverse plant-fungal interactions.
TL;DR: It is demonstrated that breakdown products of the cell wall released by the localized application of hemicellulase elicit localized responses including, sequentially, extracellular H2O2 generation; accumulation of phenolic compounds; and cross-linking of proteins in thecell wall.
References
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Journal ArticleDOI
Metachromasy: an experimental and theoretical reevaluation.
John A. Bergeron,Marcus Singer +1 more
TL;DR: It is shown that the destruction of color is a characteristic of metachromasy and that water molecules intercalated between approximated dye ions are responsible for the loss and change of color.
Journal ArticleDOI
Improved polyester wax embedding for histology.
TL;DR: Tissue structure is better preserved in polyester than in paraffin wax, probably because structural lipids are better retained and localized, and this difference between waxes is slight if tissues are well fixed and ...