Potassium-Dependant Mutants of Escherichia coli K-12
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TLDR
Mutants of Escherichia coli K-12 that grow more slowly in media containing low concentrations of K have been isolated and are therefore referred to as K-dependent, and the abbreviation kdp is proposed for this class of mutant.Abstract:
Mutants of Escherichia coli K-12 that grow more slowly in media containing low concentrations of K have been isolated. All independent mutants of this type which have been studied carry a mutation in a small region of the bacterial chromosome between the supE and gal loci. The growth rate of the mutants is the same as that of the parental strains in medium containing more than 1 mm K, but is only 50% that of the parent when the K concentration is reduced to 0.1 mm. The mutants do not appear to have a primary alteration in K transport, and are therefore referred to as K-dependent. The abbreviation kdp is proposed for this class of mutant.read more
Citations
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Journal ArticleDOI
Physiological and genetic responses of bacteria to osmotic stress.
TL;DR: This review is an account of the processes that mediate adaptation of bacteria to changes in their osmotic environment.
Journal ArticleDOI
Recalibrated linkage map of Escherichia coli K-12.
B J Bachmann,K B Low,A L Taylor +2 more
TL;DR: This article corrects the article on p. 116 in vol.
Journal ArticleDOI
Potassium Transport Loci in Escherichia coli K-12
Wolfgang Epstein,Byung S. Kim +1 more
TL;DR: Mutants of Escherichia coli K-12 requiring considerably elevated concentrations of potassium for growth are readily obtained as double mutants combining a kdp mutation with a mutation in one or more of five other loci, referred to as trk, for transport of K, because these mutations result in alterations in K transport.
Journal ArticleDOI
pH homeostasis in Escherichia coli: measurement by 31P nuclear magnetic resonance of methylphosphonate and phosphate
TL;DR: The pH-sensitive NMR probe methylphosphonate was employed, which was taken up by glycerol-grown cells and was nontoxic; its pKa of 7.65 made it an ideal probe for measurement of cytoplasmic pH and alkaline external pH.
References
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