Figure 1. Comparison of percentile rank peptide-MHC binding scores for 15mer peptides spanning the N501 or N501Y position in the spike protein. The blue background indicates improved binding affinity (lower percentile rank) for a N501 peptide versus the corresponding N501Y mutated peptide, and a pink background indicates poorer binding affinity (higher percentile rank) for a N501 vs N501Y peptide. Scatterplots are shown for (A) all available 5620 MHC-II alleles, and (B) the 1911 most common MHC-II alleles. (C) Distribution of N501Y minus N501 MHC-II presentation scores across all MHC-II alleles. (D-E) Heatmaps for all possible single amino acid substitutions at position N501, showing the percent of MHC-II alleles that have (D) worsened binding, or (E) can no longer present the mutated peptide at a threshold of 20.
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