PQM-1 Complements DAF-16 as a Key Transcriptional Regulator of DAF-2-Mediated Development and Longevity
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407 citations
Cites background from "PQM-1 Complements DAF-16 as a Key T..."
...Most of the Class 2 genes, and some of the Class 1 genes, are regulated by the zinc finger transcription factor PQM-1, which acts in opposition to DAF-16/FoxO (Tepper et al., 2013) (Section 6....
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..., 2010), and ChIP-Seq (Niu et al., 2011; Tepper et al., 2013), have revealed a large complement of DAF-16/FoxO targets (discussed in greater detail in Murphy, 2006)....
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...The functions of most of these genes and their roles in normal development and life span control are not yet understood, but recent GO analysis suggests that many of these genes regulate growth and development (Tepper et al., 2013), and reducing expression of some of these genes by RNAi extends life span (Murphy et al....
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...While PQM-1 is regulated by IIS, DAF-16 and PQM-1 also have an influence on the nuclear localization of one another, as the loss of each increases the nuclear occupancy of the other (Tepper et al., 2013)....
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...In fact, ChIP-Seq data implicates the zinc finger transcription factor PQM-1 as the DAE-binding factor, acting antagonistically with DAF-16/FoxO (Tepper et al., 2013) (Section 6....
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290 citations
Additional excerpts
...org) [30, 31], which were mapped to transcription start sites (according to [32]); and the KEGG pathway release 65....
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191 citations
165 citations
Cites background from "PQM-1 Complements DAF-16 as a Key T..."
...Understanding how neuronal CRTC-1 interacts with DAF-16 and/or PQM-1 and if they function intra- or intercellularly to modulate AMPK/ calcineurin-mediated longevity will be informative....
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...The motif strongly resembles the GATA-like DAE (DAF-16 Associated Element), recently identified as the binding site for PQM-1 (Tepper et al., 2013). modENCODE ChIP-seq data also suggests that both DAF-16 and PQM-1 bind the acs2 promoter directly....
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...We saw enrichment of the DAE element in genes downregulated when both AMPK and CRTC-1 were active, suggesting CRTC-1 might Cell 160, 842–855, February 26, 2015 ª2015 Elsevier Inc. 853 remotely regulate DAF-16/FOXO activity and/or activate its transacting antagonist PQM-1 (Tepper et al., 2013)....
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...These data therefore suggest that DAF16 and/or PQM-1 might be acting downstream of the neuronal CRTC-1 signal to modulate metabolic gene expression....
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159 citations
Cites background or methods or result from "PQM-1 Complements DAF-16 as a Key T..."
...For overlaps between FOXO binding and differential expression, genes were ranked by fold change (Foxo1+/+ vs Foxo1 / for mouse data) or FOXO/DAF-16 responsiveness in C. elegans (Tepper et al., 2013)....
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...Only a subset of the FOXO/DAF-16 targets change in expression with age, consistent with previous studies (Tepper et al., 2013)....
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...We used a meta-analysis of FOXO/DAF-16 gene expression datasets (Tepper et al., 2013) as well as microarray expression datasets from mouse cell types where ChIP-seq has been performed....
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...The enrichment for GATA family factors is notable as this family includes ELT factors and PQM-1, and these factors have been previously implicated in aging in C. elegans (Budovskaya et al., 2008; Tepper et al., 2013; Zhang et al., 2013)....
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...By interrogating the existing meta-analysis of DAF-16-transcribed genes in C. elegans (Tepper et al., 2013) (comparing daf-2 mutants vs. daf-2; daf16 mutants), we observed that DAF-16 binding is enriched at the genes most upregulated in conditions that activate DAF-16 (Fig....
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References
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"PQM-1 Complements DAF-16 as a Key T..." refers methods in this paper
...elegans Genetics All strains were cultured using standard methods (Brenner, 1974)....
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...C. elegans Genetics All strains were cultured using standard methods (Brenner, 1974)....
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4,919 citations
3,146 citations
"PQM-1 Complements DAF-16 as a Key T..." refers background in this paper
...This effect is almost entirely dependent on activation of the FOXO transcription factor DAF-16 (Kenyon et al., 1993; Lin et al., 1997; Ogg et al., 1997)....
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2,583 citations
"PQM-1 Complements DAF-16 as a Key T..." refers methods in this paper
...Survival Analysis Day 1 of adulthoodwas defined as t = 0, and the log-rank (Mantel-Cox)method was used to test the null hypothesis in Kaplan-Meier survival analysis (Lawless, 1982) and evaluated using OASIS survival analysis software (Yang et al., 2011)....
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