Preimplantation development of in vitro-matured and in vitro-fertilized ovine zygotes: comparison between coculture on oviduct epithelial cell monolayers and culture under low oxygen atmosphere.
Andrew J. Watson,Patricia H. Watson,Deirdre Warnes,Simon K. Walker,David T. Armstrong,David T. Armstrong,Robert F. Seamark +6 more
Reads0
Chats0
TLDR
Two distinct culture environments are characterized, each capable of supporting the development of high frequencies of unselected IVMF zygotes to the blastocyst stage in vitro, and survival of the embryo under a reduced oxygen atmosphere is investigated.Abstract:
The roles of medium composition, serum source, embryo coculture, and culture under low O2 conditions on the development of in vitro-matured and in vitro-fertilized (IVMF) ovine zygotes were investigated in three separate experiments. In the first experiment, the proportion of cocultured IVMF zygotes developing to the blastocyst stage was significantly higher (38.0% vs. 3.5%; p < 0.05) than that of non-cocultured zygotes treated within three embryo culture media (TCM-199 + 10% fetal bovine serum [FBS]; bicarbonate-buffered, glucose-free synthetic oviduct fluid medium [mod-SOFM] + 10% FBS; and bicarbonate-buffered BSA-free Tyrode's salt solution [mod-TALP] + 10% FBS) under a 5% CO2 atmosphere in air. In a second experiment, a significantly higher (p < 0.05) proportion of cocultured zygotes placed in TCM-199 medium survived to the blastocyst stage (37.4% blastocysts vs. 23.4% in mod-SOFM). No significant effect of serum (FBS vs. human serum [HS]) was observed on embryonic development, but coculture was confirmed to exert a significant influence on development to the blastocyst stage. In the final experiment, survival of the embryo under a reduced oxygen (5% CO2:5% O2:90% N2) atmosphere was investigated. In contrast to results in the initial experiments, embryonic survival was significantly higher (p < 0.05) in the non-cocultured treatment groups (21.9% blastocysts vs. 0.4% for cocultured zygotes). Serum source also had a significant (p < 0.05) influence upon the development of non-cocultured zygotes: 32.3% of zygotes cultured with HS progressed to the blastocyst stage vs. 11.5% of zygotes cultured in FBS-supplemented medium. These results have characterized two distinct culture environments, each capable of supporting the development of high frequencies of unselected IVMF zygotes to the blastocyst stage in vitro.read more
Citations
More filters
Embryo-maternal interactions leading to embryonic development and survival in the bovine : role of progesterone and prostaglandins
TL;DR: Conclusions were that: i) treatment with hCG induced formation of secondary CL, increased plasma P4 concentrations, survival rate of demi-embryos and pregnancy rate of recipients (only in cows), and treatment with carprofen had no significant effect on plasma P 4 concentrations and embryonic survival, but decreased the luteotrophic effect of hCG.
Dissertation
Oxygen concentration during oocyte maturation in the mouse.
TL;DR: Thesis (Ph.D.) -- University of Adelaide, School of Paediatrics and Reproductive Health, 2009.
Patent
Bioreactor for selectively controlling the molecular diffusion between fluids
TL;DR: In this paper, a diffusion controlling bioreactor (50) that selectively controls the molecular diffusion between fluids through a microchannel (200) in fluid communication with a reaction reservoir (300) is presented.
DissertationDOI
The Effects of Equine Somatotropin (eST) on Reproductive Function in the Domestic Mare.
TL;DR: In this article , the effects of exogenous equine somatotropin on ovarian development and plasma and follicular fluid hormone concentrations in cyclic and noncyclic mares were determined.
Chapter 2 In Vitro Fertilization (IVF)
TL;DR: The first IVF of a mammalian oocyte, confirmed cytologically, was performed in a rabbit using spermatozoa that had capacitated in the uterus and recently ovulated oocytes, and IVF was achieved using oocytes that had been matured in vitro in various species.
References
More filters
Journal ArticleDOI
Regression Models for Ordinal Data
TL;DR: In this article, a general class of regression models for ordinal data is developed and discussed, which utilize the ordinal nature of the data by describing various modes of stochastic ordering and this eliminates the need for assigning scores or otherwise assuming cardinality instead of ordinality.
Journal ArticleDOI
Bovine in vitro fertilization with frozen-thawed semen
John J. Parrish,J.L. Susko-Parrish,M.L. Leibfried-Rutledge,E.S. Critser,W.H. Eyestone,N. L. First +5 more
TL;DR: A procedure to obtain high and repeatable fertilization frequencies for bovine in vitro fertilization (IVF) with frozen-thawed sperm was developed and Heparin was the most important factor in increasing IVF frequencies.
Journal ArticleDOI
Superoxide Radical: An Endogenous Toxicant
TL;DR: The Two Faces of Oxygen Molecular oxygen is both benign and malign and the superoxide dismutases, by catalytically scavenging 0;, provide a defense against it and against any reactive radical species which can be derived from it.
Journal ArticleDOI
An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro
TL;DR: The in-vitro development of 1-cell embryos beyond the 2-cell stage in response to the removal of glucose and the addition of glutamine to the culture medium suggests that glucose may block some essential metabolic process, and that glutamine may be a preferred energy substrate during early development for these mouse embryos.
Journal ArticleDOI
Successful culture in vitro of sheep and cattle ova
TL;DR: This communication describes the successful culture of one-cell to eight-cell sheep ova and one- cell and eight- cell cattle ova to the morula and blastocyst stages and reports a high embryo survival after transfer of cultured Ova to recipient animals.
Related Papers (5)
Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells
Fulvio Gandolfi,R. M. Moor +1 more
Successful culture in vitro of sheep and cattle ova
Co-culture of early cattle embryos to the blastocyst stage with oviducal tissue or in conditioned medium.
W. H. Eyestone,N. L. First +1 more