Present and future prospects for wheat improvement through genome editing and advanced technologies.
Shaoya Li,Chen Zhang,Jingying Li,Lei Yan,Ning Wang,Lanqin Xia +5 more
- Vol. 2, Iss: 4, pp 100211
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TLDR
It is concluded that the combination of genome editing and other molecular breeding strategies will greatly facilitate genetic improvement of wheat for sustainable global production.Abstract:
Wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) is one of the most important staple food crops in the world. Despite the fact that wheat production has significantly increased over the past decades, future wheat production will face unprecedented challenges from global climate change, increasing world population, and water shortages in arid and semi-arid lands. Furthermore, excessive applications of diverse fertilizers and pesticides are exacerbating environmental pollution and ecological deterioration. To ensure global food and ecosystem security, it is essential to enhance the resilience of wheat production while minimizing environmental pollution through the use of cutting-edge technologies. However, the hexaploid genome and gene redundancy complicate advances in genetic research and precision gene modifications for wheat improvement, thus impeding the breeding of elite wheat cultivars. In this review, we first introduce state-of-the-art genome-editing technologies in crop plants, especially wheat, for both functional genomics and genetic improvement. We then outline applications of other technologies, such as GWAS, high-throughput genotyping and phenotyping, speed breeding, and synthetic biology, in wheat. Finally, we discuss existing challenges in wheat genome editing and future prospects for precision gene modifications using advanced genome-editing technologies. We conclude that the combination of genome editing and other molecular breeding strategies will greatly facilitate genetic improvement of wheat for sustainable global production.read more
Citations
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Journal ArticleDOI
Increasing yield potential through manipulating of an ARE1 ortholog related to nitrogen use efficiency in wheat by CRISPR/Cas9
TL;DR: In this paper, the authors used CRISPR/Cas9-mediated targeted mutagenesis to generate a series of transgene-free mutant lines with partial or triple-null taare1 alleles.
Journal ArticleDOI
OUP accepted manuscript
TL;DR: In this paper , the authors summarized CRISPR/Cas-mediated HDR in plants and described diverse strategies to improve HDR efficiency by manipulating the DNA repair pathway, timing DSB induction, and donor delivery.
Journal ArticleDOI
An update on precision genome editing by homology-directed repair in plants.
TL;DR: Diverse strategies to improve HDR efficiency by manipulating the DNA repair pathway, timing DSB induction and donor delivery, etc are described, which outline open questions and challenges in HDR-mediated precision genome editing in both plant biological research and crop improvement.
Journal ArticleDOI
Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties.
TL;DR: In this paper, the authors targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-EIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert.
Journal ArticleDOI
Wheat Biofortification: Utilizing Natural Genetic Diversity, Genome-Wide Association Mapping, Genomic Selection, and Genome Editing Technologies
TL;DR: In this paper , a review of the current understanding of availability and utilization of natural genetic variations for grain micronutrients among cultivated and wild relatives, QTLs/genes and different genomic regions regulating the accumulation of micRONutrients, and the status of mic-nutrient bio-fortified wheat varieties released for commercial cultivation across the globe.
References
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