Journal ArticleDOI
Prime genome editing in rice and wheat
Qiupeng Lin,Yuan Zong,Chenxiao Xue,Shengxing Wang,Shuai Jin,Zixu Zhu,Yanpeng Wang,Andrew V. Anzalone,Andrew V. Anzalone,Andrew V. Anzalone,Aditya Raguram,Aditya Raguram,Aditya Raguram,Jordan L. Doman,Jordan L. Doman,Jordan L. Doman,David R. Liu,David R. Liu,David R. Liu,Caixia Gao +19 more
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TLDR
The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts through codon, promoter, and editing-condition optimization.Abstract:
Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.read more
Citations
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Journal ArticleDOI
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Andrew V. Anzalone,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,David R. Liu,David R. Liu,David R. Liu +6 more
TL;DR: This work analyzes key considerations when choosing genome editing agents and identifies opportunities for future improvements and applications in basic research and therapeutics.
Journal ArticleDOI
Applications of CRISPR–Cas in agriculture and plant biotechnology
Haocheng Zhu,Chao Li,Caixia Gao +2 more
TL;DR: The most important applications of CRISPR-Cas in increasing plant yield, quality, disease resistance and herbicide resistance, breeding and accelerated domestication, and prospective applications of this game-changing technology are discussed.
Journal ArticleDOI
Genome engineering for crop improvement and future agriculture
TL;DR: This article reviewed the development and application of genome editing tools in plants while highlighting newly developed techniques and discussed their impact on crop production, with an emphasis on recent advancements in genome editing-based plant improvements that could not be achieved by conventional breeding.
Journal ArticleDOI
Enhanced prime editing systems by manipulating cellular determinants of editing outcomes
Peter J. Chen,Peter J. Chen,Peter J. Chen,Jeffrey A. Hussmann,Jun Yan,Friederike Knipping,Purnima Ravisankar,Pin-Fang Chen,Cidi Chen,James W. Nelson,James W. Nelson,James W. Nelson,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Mustafa Sahin,Mustafa Sahin,Mark J. Osborn,Jonathan S. Weissman,Jonathan S. Weissman,Britt Adamson,David R. Liu,David R. Liu,David R. Liu +23 more
TL;DR: In this paper, the DNA mismatch repair (MMR) impedes prime editing and promotes undesired indel byproducts, and the authors developed PE4 and PE5 prime editing systems in which transient expression of an engineered MMR-inhibiting protein enhances the efficiency of prime edits.
Journal ArticleDOI
PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants.
TL;DR: PnB Designer as discussed by the authors is a web-based application for the design of pegRNAs for PEs and guide RNAs for BEs, which can be used to model all known disease causing mutations available in ClinVar.
References
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Journal ArticleDOI
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Alexis C. Komor,Yongjoo Kim,Yongjoo Kim,Michael S. Packer,Michael S. Packer,John A. Zuris,John A. Zuris,David R. Liu,David R. Liu +8 more
TL;DR: E engineered fusions of CRISPR/Cas9 and a cytidine deaminase enzyme that retain the ability to be programmed with a guide RNA, do not induce dsDNA breaks, and mediate the direct conversion of cytidine to uridine, thereby effecting a C→T (or G→A) substitution.
Journal ArticleDOI
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
Nicole M. Gaudelli,Alexis C. Komor,Holly A. Rees,Holly A. Rees,Holly A. Rees,Michael S. Packer,Ahmed H. Badran,Ahmed H. Badran,Ahmed H. Badran,David I. Bryson,David R. Liu,David R. Liu,David R. Liu +12 more
TL;DR: Adenine base editors (ABEs) that mediate the conversion of A•T to G•C in genomic DNA are described and a transfer RNA adenosine deaminase is evolved to operate on DNA when fused to a catalytically impaired CRISPR–Cas9 mutant.
Journal ArticleDOI
Search-and-replace genome editing without double-strand breaks or donor DNA
Andrew V. Anzalone,Andrew V. Anzalone,Andrew V. Anzalone,Peyton B. Randolph,Peyton B. Randolph,Peyton B. Randolph,Jessie Rose Davis,Jessie Rose Davis,Jessie Rose Davis,Alexander A. Sousa,Alexander A. Sousa,Alexander A. Sousa,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,Jonathan M. Levy,Jonathan M. Levy,Jonathan M. Levy,Peter J. Chen,Peter J. Chen,Peter J. Chen,Christine D. Wilson,Christine D. Wilson,Christine D. Wilson,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Aditya Raguram,Aditya Raguram,Aditya Raguram,David R. Liu,David R. Liu,David R. Liu +32 more
TL;DR: A new DNA-editing technique called prime editing offers improved versatility and efficiency with reduced byproducts compared with existing techniques, and shows potential for correcting disease-associated mutations.
Journal ArticleDOI
Targeted genome modification of crop plants using a CRISPR-Cas system.
Qiwei Shan,Yanpeng Wang,Jun Li,Yi Zhang,Kunling Chen,Zhen Liang,Kang Zhang,Jinxing Liu,Jianzhong Jeff Xi,Jin-Long Qiu,Caixia Gao +10 more
TL;DR: This work, together with the mice work, demonstrates that it should be feasible to produce genetargeted models in rodents and probably other mammalian species using the CRISPRCas systems.
Journal ArticleDOI
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew
TL;DR: It is shown that TALEN-induced mutation of all three TaMLO homoeologs in the same plant confers heritable broad-spectrum resistance to powdery mildew, and provides a methodological framework to improve polyploid crops.
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Search-and-replace genome editing without double-strand breaks or donor DNA
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