PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification
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A total of 306 800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as Tm, location on the transcript and amplicon size.Abstract:
PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306 800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as Tm, location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. There are several ways to search for primers for the gene(s) of interest, such as by: GenBank accession number, NCBI protein accession number, NCBI gene ID, PrimerBank ID, NCBI gene symbol or gene description (keyword). In all, 26 855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site.read more
Citations
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Journal ArticleDOI
Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction
TL;DR: A new software tool called Primer-BLAST is presented to alleviate the difficulty in designing target-specific primers and combines BLAST with a global alignment algorithm to ensure a full primer-target alignment and is sensitive enough to detect targets that have a significant number of mismatches to primers.
Journal ArticleDOI
The transcription factor Myc controls metabolic reprogramming upon T lymphocyte activation
Ruoning Wang,Christopher P. Dillon,Lewis Zhichang Shi,Sandra Milasta,Robert Carter,David Finkelstein,Laura L. McCormick,Patrick Fitzgerald,Hongbo Chi,Joshua Munger,Douglas R. Green +10 more
TL;DR: Metabolic tracer analysis revealed a Myc-dependent metabolic pathway linking glutaminolysis to the biosynthesis of polyamines, which may represent a general mechanism for metabolic reprogramming under patho-physiological conditions.
Journal ArticleDOI
Gut microbes promote colonic serotonin production through an effect of short-chain fatty acids on enterochromaffin cells
Christopher S. Reigstad,Charles E. Salmonson,John F. Rainey,Joseph H. Szurszewski,David R. Linden,Justin L. Sonnenburg,Gianrico Farrugia,Purna C. Kashyap +7 more
TL;DR: Gut microbiota acting through SCFAs are important determinants of enteric 5‐HT production and homeostasis through an effect of short‐chain fatty acids on enterochromaffin cells.
Journal ArticleDOI
PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update.
TL;DR: This work describes a major update of PrimerBank, which contains primers that can be used for PCR and qPCR under stringent and allele-invariant amplification conditions, and includes the design of new primers covering 17 076 and 18 086 genes for the human and mouse species.
Journal ArticleDOI
Dedifferentiation of neurons and astrocytes by oncogenes can induce gliomas in mice.
Dinorah Friedmann-Morvinski,Eric A. Bushong,Eugene Ke,Eugene Ke,Yasushi Soda,Tomotoshi Marumoto,Tomotoshi Marumoto,Oded Singer,Mark H. Ellisman,Inder M. Verma +9 more
TL;DR: It is proposed that most differentiated cells in the CNS upon defined genetic alterations undergo dedifferentiation to generate a NSC or progenitor state to initiate and maintain the tumor progression, as well as to give rise to the heterogeneous populations observed in malignant gliomas.
References
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