Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp . ATCC 31750
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A mutant that produced higher levels of curdlan than the wild strain Agrobacterium sp.Abstract:
We isolated a mutant that produced higher levels of curdlan than the wild strain Agrobacterium sp. ATCC 31750 by chemical mutagenesis using N-methyl-N-nitro-nitrosoguanidine. The mutant strain produced 66 g/L of curdlan in 120 h with a yield of (0.88) while, the wild strain produced 41 g/L in 120 h with a yield of (0.62) in a stirred bioreactor. The mutant could not produce curdlan when the pH was shifted from 7.0 to 5.5 after nitrogen depletion as followed for wild strain. In contrast, pH optimum for cell growth and curdlan production for mutant was found to be 7.0. We optimized the downstream processing of curdlan by varying different volumes of NaOH and HCl for extraction and precipitation of curdlan. The molecular weight of the purified curdlan from the wild and mutant strain was 6.6 × 105 Da and 5.8 × 105 Da respectively. The monosaccharide analyses confirm that curdlan from both wild and mutant strain contains only glucose units. From the NMR and FTIR data, it has been confirmed that curdlan was exclusively composed of β (1 → 3)-D-glucan residues.read more
Citations
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References
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Improved production of curdlan with concentrated cells ofAgrobacterium sp.
TL;DR: In this paper, the addition of a limited concentration of yeast extract to a minimal salt medium (MSM) enhanced cell growth and increased the production of curdlan whereas nitrogenlimitation was found to be essential for the higher production of Curdlan by Agrobacterium sp.
Journal ArticleDOI
Curdlan-like exopolysaccharide production by Cellulomonas flavigena UNP3 during growth on hydrocarbon substrates
TL;DR: Cellulomonas flavigena UNP3, a natural isolate from vegetable oil contaminated soil sample has been studied for growth associated exopolysaccharide (EPS) production during growth on glucose, groundnut oil and naphthalene to confirm the role of EPS as bioemulsifier.
Journal ArticleDOI
Effect of medium pH and cultivation period on mycelial biomass, polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum from Montenegro
TL;DR: The effect of initial medium pH on biomass, extracellular and intracellular polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum was investigated at different pH values after 7 and 14 days of cultivation.
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