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Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )

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TLDR
Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Abstract
Homozygous diploid zebra fish have been produced on a large scale by the application of simple physical treatments. Clones of homozygous fish have been produced from individual homozygotes. These clones and associated genetic methods will facilitate genetic analyses of this vertebrate.

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Journal ArticleDOI

Stages of embryonic development of the zebrafish.

TL;DR: A series of stages for development of the embryo of the zebrafish, Danio (Brachydanio) rerio is described, providing for flexibility and continued evolution of the staging series as the authors learn more about development in this species.
Journal ArticleDOI

The zebrafish reference genome sequence and its relationship to the human genome.

Kerstin Howe, +174 more
- 25 Apr 2013 - 
TL;DR: A high-quality sequence assembly of the zebrafish genome is generated, made up of an overlapping set of completely sequenced large-insert clones that were ordered and oriented using a high-resolution high-density meiotic map, providing a clearer understanding of key genomic features such as a unique repeat content, a scarcity of pseudogenes, an enrichment of zebra fish-specific genes on chromosome 4 and chromosomal regions that influence sex determination.
Journal ArticleDOI

Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences

TL;DR: The lability of sex-determination systems in fish makes some species sensitive to environmental pollutants capable of mimicking or disrupting sex hormone actions, and such observations provide important insight into potential impacts from endocrine disruptors, and can provide useful monitoring tools for impacts on aquatic environments.
Journal ArticleDOI

Animal models of human disease: zebrafish swim into view.

TL;DR: This Review surveys the achievements and potential of zebrafish for modelling human diseases and for drug discovery and development.
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The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio

TL;DR: It is estimated that the 372 genes defined by the mutants probably represent more than half of all genes that could have been discovered using the criteria of the screen, and the limits and the potentials of a genetic saturation screen in the zebrafish.
References
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Journal ArticleDOI

The genetics of caenorhabditis elegans

TL;DR: In this paper, the authors describe methods for the isolation, complementation and mapping of mutants of Caenorhabditis elegans, a small free-living nematode worm.
Journal Article

The genetics of Caenorhabditis elegans.

Daniel S. Brenner, +1 more
- 29 Apr 1974 - 
TL;DR: Estimates of the induced mutation frequency of both the visible mutants and X chromosome lethals suggests that, just as in Drosophila, the genetic units in C. elegans are large.
Journal ArticleDOI

Starch gel electrophoresis of enzymes—A compilation of recipes

TL;DR: Most of the methods outlined here have been used primarily for tissue extracts from mammalian species, and a few have been developed only on plants or lower animals, but this is not to say that they will not work on higher organisms.
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BEHAVIORAL MUTANTS OF Drosophila ISOLATED BY COUNTERCURRENT DISTRIBUTION

TL;DR: Drosophila offers the same advantages to such a study as it did to classical genetics, namely, large numbers and short generation time, to which may now be added an enormous store of accumulated knowledge concerning the organism.
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