Production of ethanol and arabitol by Debaryomyces nepalensis : influence of process parameters

doi:10.1186/2191-0855-3-23

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Production of ethanol and arabitol by Debaryomyces nepalensis : influence of process parameters

Journal Article•DOI•

Production of ethanol and arabitol by Debaryomyces nepalensis : influence of process parameters

Himabindu Kumdam¹, Shweta Narayana Murthy¹, Sathyanarayana N. Gummadi¹•Institutions (1)

Indian Institute of Technology Madras¹

09 May 2013-AMB Express (SpringerOpen)-Vol. 3, Iss: 1, pp 23-23

TL;DR: Debaryomyces nepalensis, osmotolerant yeast isolated from rotten apple, is known to utilize both hexoses and pentoses and produce industrially important metabolites like ethanol, xylitol and arabitol.

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Abstract: Debaryomyces nepalensis, osmotolerant yeast isolated from rotten apple, is known to utilize both hexoses and pentoses and produce industrially important metabolites like ethanol, xylitol and arabitol. In the present study, the effect of different growth substrates, trace elements, nitrogen concentration and initial pH on growth and formation of ethanol and arabitol were examined. Optimum conditions for maximizing the product yields were established: glucose as carbon source, an initial pH of 6.0, 6 g/L of ammonium sulphate and addition of micronutrients. Under these best suited conditions, a concentration of 11g/L of arabitol and 19 g/L of ethanol was obtained in shake flask fermentations. The fermentation was scaled up to 2.5 L bioreactor and the influence of aeration, agitation and initial substrate concentration was also determined. Under optimal conditions (150 g/L glucose, 400 rpm and 0.5 vvm) ethanol concentration reached 52 g/L, which corresponds to a yield of 0.34 g/g and volumetric productivity of 0.28 g/L/h, whereas arabitol production reached a maximum of 14 g/L with a yield and volumetric productivity of 0.1 g/g and 0.07 g/L/h respectively.

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Journal Article•DOI•

Production of arabitol by yeasts: current status and future prospects

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M. Kordowska-Wiater¹•Institutions (1)

University of Life Sciences in Lublin¹

01 Aug 2015-Journal of Applied Microbiology

TL;DR: The present review discusses research on native yeasts from the genera Candida, Pichia, Debaryomyces and Zygosaccharomyces as well as genetically modified strains of SacCharomyces cerevisiae which are able to utilize biomass hydrolysates to effectively produce l‐ or d‐arabitol.

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Abstract: Arabitol belongs to the pentitol family and is used in the food industry as a sweetener and in the production of human therapeutics as an anticariogenic agent and an adipose tissue reducer. It can also be utilized as a substrate for chemical products such as arabinoic and xylonic acids, propylene, ethylene glycol, xylitol and others. It is included on the list of 12 building block C3-C6 compounds, designated for further biotechnological research. This polyol can be produced by yeasts in the processes of bioconversion or biotransformation of waste materials from agriculture, the forest industry (l-arabinose, glucose) and the biodiesel industry (glycerol). The present review discusses research on native yeasts from the genera Candida, Pichia, Debaryomyces and Zygosaccharomyces as well as genetically modified strains of Saccharomyces cerevisiae which are able to utilize biomass hydrolysates to effectively produce L- or D-arabitol. The metabolic pathways of these yeasts leading from sugars and glycerol to arabitol are presented. Although the number of reports concerning microbial production of arabitol is rather limited, the research on this topic has been growing for the last several years, with researchers looking for new micro-organisms, substrates and technologies.

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64 citations

Cites background from "Production of ethanol and arabitol ..."

...Currently, arabitol is produced on an industrial scale by the chemical reduction in lactones of arabinonic and lyxonic acids, a reaction that requires an expensive catalyst and a constant temperature of 100°C (Kumdam et al. 2013)....
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...Its efficacy is similar to that of soluble dietary fibres (Mingguo et al. 2011; Kumdam et al. 2013)....
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...Ethanol was a second product, but only a very small amount of that (2 43 g l 1) was detected in the broth and the yield obtained was 0 03 g g 1 (Kumdam et al. 2013)....
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...Its efficacy is similar to that of soluble dietary fibres (Mingguo et al. 2011; Kumdam et al. 2013)....
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...Ethanol was a second product, but only a very small amount of that (2 43 g l (1)) was detected in the broth and the yield obtained was 0 03 g g 1 (Kumdam et al. 2013)....
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Journal Article•DOI•

Artificial neural network and regression coupled genetic algorithm to optimize parameters for enhanced xylitol production by Debaryomyces nepalensis in bioreactor

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Sharon Mano J. Pappu¹, Sathyanarayana N. Gummadi¹•Institutions (1)

Indian Institute of Technology Madras¹

15 Apr 2017-Biochemical Engineering Journal

TL;DR: In this paper, a multi-layer feed forward neural network (ANN) of 5-10-2 topology has been developed to predict the xylitol production and genetic algorithm (GA) was used to find the optimum parameters to enhance xylanol production.

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61 citations

Journal Article•DOI•

Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel-derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid.

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Rosanina Filippousi¹, Dimitrios Antoniou¹, Paschalitsa Tryfinopoulou¹, Aspasia Nisiotou, George-John E. Nychas¹, Apostolis A. Koutinas¹, Seraphim Papanikolaou¹ - Show less +3 more•Institutions (1)

Agricultural University of Athens¹

01 Oct 2019-Journal of Applied Microbiology

TL;DR: To assess the ability of various newly isolated or belonging yeast strains to convert biodiesel‐derived glycerol (Gly) into added‐value compounds, yeast strains from official collections are tested.

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Abstract: Aims To assess the ability of various newly isolated or belonging in official collections yeast strains to convert biodiesel-derived glycerol (Gly) into added-value compounds. Methods and results Ten newly isolated yeast strains belonging to Debaryomyces sp., Naganishia uzbekistanensis, Rhodotorula sp. and Yarrowia lipolytica, isolated from fishes, metabolized Gly under nitrogen limitation. The aim of the study was to identify potential newly isolated microbial candidates that could produce single-cell oil (SCO), endopolysaccharides and polyols when these micro-organisms were grown on biodiesel-derived Gly. As controls producing SCO and endopolysaccharides were the strains Rhodotorula glutinis NRRL YB-252 and Cryptococcus curvatus NRRL Y-1511. At initial Gly (Gly0 ) ≈40 g l-1 , most strains presented remarkable dry cell weight (DCW) production, whereas Y. lipolytica and Debaryomyces sp. produced non-negligible quantities of mannitol and arabitol (Ara). Five strains were further cultivated at increasing Gly0 concentrations. Rhodotorula glutinis NRRL YB-252 produced 7·2 g l-1 of lipid (lipid in DCW value ≈38% w/w), whereas Debaryomyces sp. FMCC Y69 in batch-bioreactor experiment with Gly0 ≈80 g l-1 , produced 30-33 g l-1 of DCW and ~30 g l-1 of Ara. At shake-flasks with Gly0 ≈125 g l-1 , Ara of ~48 g l-1 (conversion yield of polyol on Gly consumed ≈0·62 g g-1 ) was achieved. Cellular lipids of all yeasts contained in variable concentrations oleic, palmitic, stearic and linoleic acids. Conclusions Newly isolated, food-derived and non-previously studied yeast isolates converted biodiesel-derived Gly into several added-value metabolites. Significance and impact of the study Alternative ways of crude Gly valorization through yeast fermentations were provided and added-value compounds were synthesized.

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37 citations

Cites result from "Production of ethanol and arabitol ..."

...Arabitol production achieved compares favorably with most of the literature reports presented in the literature concerning Ara production by yeasts (Kordowska-Wiater et al. 2008; Kordowska-Wiater et al. 2017; Koganti et al. 2011; Koganti and Ju 2013; Kumdam et al. 2013; Kordowska-Wiater 2015)....
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...…of fermentation) demonstrated maximum Ara concentrations lower or in the same magnitude with the results achieved in the current investigation; i.e. Kumdam et al. (2013) determined that Ara was produced from L-arabinose as the sole carbon source at a concentration of 22 7 g l 1 and a yield of 0…...
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Journal Article•DOI•

Production of arabitol from enzymatic hydrolysate of soybean flour by Debaryomyces hansenii fermentation

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Abdullah Al Loman¹, S. M. M. Islam¹, Lu-Kwang Ju¹•Institutions (1)

University of Akron¹

01 Jan 2018-Applied Microbiology and Biotechnology

TL;DR: Arabitol yield increased with increasing C/N ratio and a high I/O-N, suggesting higher yield at stationary phase of low pH, and catabolite repression was observed, with the following order of consumption: glucose > fructose > galactose > xylose > arabinose.

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Abstract: Arabitol is a low-calorie sugar alcohol with anti-cariogenic properties. Enzymatic hydrolysate of soybean flour is a new renewable biorefinery feedstock containing hexose, pentose, and organic nitrogen sources. Arabitol production by Debaryomyces hansenii using soybean flour hydrolysate was investigated. Effects of medium composition, operating conditions, and culture stage (growing or stationary phase) were studied. Production was also compared at different culture volumes to understand the effect of dissolved oxygen concentration (DO). Main factors examined for medium composition effects were the carbon to nitrogen concentration ratio (C/N), inorganic (ammonium) to organic nitrogen ratio (I/O-N), and sugar composition. Arabitol yield increased with increasing C/N ratio and a high I/O-N (0.8–1.0), suggesting higher yield at stationary phase of low pH (3.5–4.5). Catabolite repression was observed, with the following order of consumption: glucose > fructose > galactose > xylose > arabinose. Arabitol production also favored hexoses and, among hexoses, glucose. DO condition was of critical importance to arabitol production and cell metabolism. The yeast consumed pentoses (xylose and arabinose) only at more favorable DO conditions. Finally, arabitol was produced in fermentors using mixed hydrolysates of soy flour and hulls. The process gave an arabitol yield of 54%, volumetric productivity of 0.90 g/L-h, and specific productivity of 0.031 g/g-h.

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27 citations

Journal Article•DOI•

Enhanced d-arabitol production by Zygosaccharomyces rouxii JM-C46: isolation of strains and process of repeated-batch fermentation

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Xianghui Qi¹, Yan Luo¹, Xu Wang¹, Jingfei Zhu¹, Jing Lin¹, Huanhuan Zhang¹, Fang Chen¹, Wenjing Sun¹ - Show less +4 more•Institutions (1)

Jiangsu University¹

25 Feb 2015-Journal of Industrial Microbiology & Biotechnology

TL;DR: A new strain producing high yield of d-arabitol was isolated from hyperosmotic environments and the ITS rDNA sequencing analysis revealed it as Zygosaccharomyces rouxii JM-C46, which has potential to be used for d-Arabitol and xylitol production from glucose via d-ARabitol route.

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Abstract: A new strain producing high yield of d-arabitol was isolated from hyperosmotic environments and the ITS rDNA sequencing analysis revealed it as Zygosaccharomyces rouxii. In addition, using a pH control and repeated-batch fermentation strategy in a 5-L reactor, the maximum yield and the highest volumetric productivity of d-arabitol were 93.48 ± 2.79 g/L and 1.143 g/L h, respectively. Volumetric productivity was successfully improved from 0.86 to 1.143 g/L h, which was increased by 32.9 % after 72 h of fermentation. Z. rouxii JM-C46 has potential to be used for d-arabitol and xylitol production from glucose via d-arabitol route.

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24 citations

Cites background from "Production of ethanol and arabitol ..."

...The production of d-arabitol from d-glucose using osmophilic yeasts belonging to the genera Saccharomycopsis [19], Candida [14], Debaryomyces [9], Pichia [3], Hansenula [16], Metschnikowia [12], and Zygosaccharomyces [5] has been reported....
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Journal Article•

Optimization of C/N ratio of the medium and fermentation conditions of ethanol production from tapioca starch using Co-Culture of Aspergillus niger and Sachormyces cerevisiae.

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K. Manikandan, T. Viruthagiri

01 Jan 2010-International Journal of ChemTech Research

TL;DR: In this article, the effect of pH, temperature and substrate concentration on ethanol yield from pretreated tapioca flour and the above parameters were optimized using response surface methodology (RSM) to obtain the optimum values of 5.5, 30°C and 60 g/l respectively.

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Abstract: Studies on Co-Culture fermentation of tapioca flour as the substrate symbiotic strains of starch digesting Aspergillus niger and non starch digesting and sugar fermenting Saccharomyces Cerevisaie in a batch fermentation. The effect of Carbon to Nitrogen (C/N) ratio of the fermentation medium on ethanol concentration and biomass was investigated. The optimum C/N ratio of the fermentation medium was found to be 35.2.which gave a maximum ethanol concentration of 8.85 g/l. Experiments based on Central Composite Design (CCD) were conducted to study the effect of pH, temperature and substrate concentration on ethanol yield from pretreated tapioca flour and the above parameters were optimized using response surface methodology (RSM). The optimum values of pH, temperature and substrate concentration were found to be 5.5, 30°C and 60 g/l respectively. The tapioca flour solution equivalent to 6% initial starch concentration gave the highest ethanol concentration of 8.9 g/l after 48 h of fermentation at optimum conditions of pH and temperature. Logistic model was used for growth kinetics and Leudeking – Piret model was used for product formation kinetics.

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32 citations

"Production of ethanol and arabitol ..." refers background in this paper

...It was also reported that decreasing C/N ratio resulted in poor ethanol yield (Manikandan and Viruthagiri 2010)....
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Journal Article•DOI•

D-Arabitol Production by Endomycopsis chodati

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G. J. Hajny¹•Institutions (1)

United States Department of Agriculture¹

01 Jan 1964-Applied and Environmental Microbiology

TL;DR: A synthetic medium was developed for the fermentation that showed that nitrogen in the medium must be limiting to obtain high yields of arabitol, and endedomycopsis chodati in an aerated fermentation produced d-arabitol in yields of 35 to 40% of the sugar supplied.

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Abstract: Endomycopsis chodati in an aerated fermentation produced d-arabitol in yields of 35 to 40% of the sugar supplied. Glucose, mannose, and sucrose were suitable substrates. A synthetic medium was developed for the fermentation that showed that nitrogen in the medium must be limiting to obtain high yields of arabitol. Excess phosphate also tended to lower arabitol yields, although the effect was not so great as with nitrogen. Pilot plant-size fermentations were made in which all the nutrients were supplied by blackstrap molasses and urea. Arabitol yields in these fermentations were about 40% of the sugar supplied.

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32 citations

"Production of ethanol and arabitol ..." refers background in this paper

...There were many reports indicating the necessity of adequate supply of oxygen for polyol production (Petrson et al. 1958; Hajny 1964; Escalante et al. 1990; Saha et al. 2007; Toyoda and Ohtaguchi 2011)....
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Journal Article•DOI•

Production of arabitol from glucose by Hansenula polymorpha

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Javier Escalante, Gloria Caminal¹, Marta Figueredo, Carles de Mas•Institutions (1)

Spanish National Research Council¹

01 Jan 1990-Journal of Fermentation and Bioengineering

TL;DR: Arabitol was produced from glucose by Hansenula polymorpha DSM 70277 and neither ethanol nor acetate were detected in this experiment.

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30 citations

"Production of ethanol and arabitol ..." refers background or result in this paper

...But, in the case of Hansenula polymorpha, there was a reduction in arabitol production with increasing osmotic pressure (Escalante et al. 1990)....
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...But, in contrast to the above observation, Escalante et al. (1990) reported an increase in arabitol yield with increasing aeration and agitation speed....
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...There were many reports indicating the necessity of adequate supply of oxygen for polyol production (Petrson et al. 1958; Hajny 1964; Escalante et al. 1990; Saha et al. 2007; Toyoda and Ohtaguchi 2011)....
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Journal Article•DOI•

Metabolism of glucose and xylose as single and mixed feed in Debaryomyces nepalensis NCYC 3413: production of industrially important metabolites

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Sawan Kumar¹, Sathyanarayana N. Gummadi¹•Institutions (1)

Indian Institute of Technology Madras¹

08 Feb 2011-Applied Microbiology and Biotechnology

TL;DR: The kinetics of growth and polyol production by Debaryomyces nepalensis NCYC 3413 was studied under single and mixed substrate conditions and the first study to elaborate the glucose and xylose metabolic pathway in this yeast strain was elaborate.

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Abstract: Efficient conversion of hexose and pentose (glucose and xylose) by a single strain is a very important factor for the production of industrially important metabolites using lignocellulose as the substrate. The kinetics of growth and polyol production by Debaryomyces nepalensis NCYC 3413 was studied under single and mixed substrate conditions. In the presence of glucose, the strain produced ethanol (35.8 ± 2.3 g/l), glycerol (9.0 ± 0.2 g/l), and arabitol (6.3 ± 0.2 g/l). In the presence of xylose, the strain produced xylitol (38 ± 1.8 g/l) and glycerol (18 ± 1.0 g/l) as major metabolites. Diauxic growth was observed when the strain was grown with different combinations of glucose/xylose, and glucose was the preferred substrate. The presence of glucose enhanced the conversion of xylose to xylitol. By feeding a mixture of glucose at 100 g/l and xylose at 100 g/l, it was found that the strain produced a maximum of 72 ± 3 g/l of xylitol. A study of important enzymes involved in the synthesis of xylitol (xylose reductase (XR) and xylitol dehydrogenase (XDH)), glycerol (glycerol-3-phosphate dehydrogenase (G3PDH)) and ethanol (alcohol dehydrogenase (ADH)) in cells grown in the presence of glucose and xylose revealed high specific activity of G3PDH and ADH in cells grown in the presence of glucose, whereas high specific activity of XR, XDH, and G3PDH was observed in cells grown in the presence of xylose. To our knowledge, this is the first study to elaborate the glucose and xylose metabolic pathway in this yeast strain.

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30 citations

"Production of ethanol and arabitol ..." refers background or methods or result in this paper

...D. nepalensis is known to survive over a wide pH range from 3.0 to 11.0 (Gummadi and Kumar 2006b, Kumar and Gummadi 2011)....
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...…metabolites arabitol, ethanol and glycerol were estimated by HPLC (Jasco) equipped with refractive index detector and Aminex HPX-87H column (Bio-Rad, Richmond, USA) at 45°C with 0.01N H2SO4 solution as the mobile phase at a flow rate of 0.6 ml/min as described earlier (Kumar and Gummadi 2011) ....
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...nepalensis produced arabitol as a compatible solute along with glycerol to overcome hyperosmotic stress (Kumar and Gummadi 2011)....
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...Debaryomyces nepalensis NCYC 3413, a halotolerant strain that has been previously isolated from rotten apple, is capable of utilizing both hexoses and pentoses; and is also important in production of commercially valuable products such as arabitol, xylitol and ethanol (Gummadi and Kumar 2006a; Kumar and Gummadi 2011; Kumdam et al. 2012)....
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...…strain that has been previously isolated from rotten apple, is capable of utilizing both hexoses and pentoses; and is also important in production of commercially valuable products such as arabitol, xylitol and ethanol (Gummadi and Kumar 2006a; Kumar and Gummadi 2011; Kumdam et al. 2012)....
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Journal Article•DOI•

Optimization of chemical and physical parameters affecting the activity of pectin lyase and pectate lyase from Debaryomyces nepalensis: A statistical approach

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Sathyanarayana N. Gummadi¹, D. Sunil Kumar¹•Institutions (1)

Indian Institute of Technology Madras¹

01 Jun 2006-Biochemical Engineering Journal

TL;DR: Debaryomyces nepalensis was previously isolated, which is capable of producing pectin lyase and pectate lyase using pect in as sole source of carbon, and its activity was increased by 1.3- and 1.4-fold after optimization.

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26 citations

"Production of ethanol and arabitol ..." refers background in this paper

...…strain that has been previously isolated from rotten apple, is capable of utilizing both hexoses and pentoses; and is also important in production of commercially valuable products such as arabitol, xylitol and ethanol (Gummadi and Kumar 2006a; Kumar and Gummadi 2011; Kumdam et al. 2012)....
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...D. nepalensis is known to survive over a wide pH range from 3.0 to 11.0 (Gummadi and Kumar 2006b, Kumar and Gummadi 2011)....
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