Production of ethanol and arabitol by Debaryomyces nepalensis : influence of process parameters

doi:10.1186/2191-0855-3-23

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Production of ethanol and arabitol by Debaryomyces nepalensis : influence of process parameters

Journal Article•DOI•

Production of ethanol and arabitol by Debaryomyces nepalensis : influence of process parameters

Himabindu Kumdam¹, Shweta Narayana Murthy¹, Sathyanarayana N. Gummadi¹•Institutions (1)

Indian Institute of Technology Madras¹

09 May 2013-AMB Express (SpringerOpen)-Vol. 3, Iss: 1, pp 23-23

TL;DR: Debaryomyces nepalensis, osmotolerant yeast isolated from rotten apple, is known to utilize both hexoses and pentoses and produce industrially important metabolites like ethanol, xylitol and arabitol.

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Abstract: Debaryomyces nepalensis, osmotolerant yeast isolated from rotten apple, is known to utilize both hexoses and pentoses and produce industrially important metabolites like ethanol, xylitol and arabitol. In the present study, the effect of different growth substrates, trace elements, nitrogen concentration and initial pH on growth and formation of ethanol and arabitol were examined. Optimum conditions for maximizing the product yields were established: glucose as carbon source, an initial pH of 6.0, 6 g/L of ammonium sulphate and addition of micronutrients. Under these best suited conditions, a concentration of 11g/L of arabitol and 19 g/L of ethanol was obtained in shake flask fermentations. The fermentation was scaled up to 2.5 L bioreactor and the influence of aeration, agitation and initial substrate concentration was also determined. Under optimal conditions (150 g/L glucose, 400 rpm and 0.5 vvm) ethanol concentration reached 52 g/L, which corresponds to a yield of 0.34 g/g and volumetric productivity of 0.28 g/L/h, whereas arabitol production reached a maximum of 14 g/L with a yield and volumetric productivity of 0.1 g/g and 0.07 g/L/h respectively.

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Journal Article•DOI•

Production of arabitol by yeasts: current status and future prospects

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M. Kordowska-Wiater¹•Institutions (1)

University of Life Sciences in Lublin¹

01 Aug 2015-Journal of Applied Microbiology

TL;DR: The present review discusses research on native yeasts from the genera Candida, Pichia, Debaryomyces and Zygosaccharomyces as well as genetically modified strains of SacCharomyces cerevisiae which are able to utilize biomass hydrolysates to effectively produce l‐ or d‐arabitol.

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Abstract: Arabitol belongs to the pentitol family and is used in the food industry as a sweetener and in the production of human therapeutics as an anticariogenic agent and an adipose tissue reducer. It can also be utilized as a substrate for chemical products such as arabinoic and xylonic acids, propylene, ethylene glycol, xylitol and others. It is included on the list of 12 building block C3-C6 compounds, designated for further biotechnological research. This polyol can be produced by yeasts in the processes of bioconversion or biotransformation of waste materials from agriculture, the forest industry (l-arabinose, glucose) and the biodiesel industry (glycerol). The present review discusses research on native yeasts from the genera Candida, Pichia, Debaryomyces and Zygosaccharomyces as well as genetically modified strains of Saccharomyces cerevisiae which are able to utilize biomass hydrolysates to effectively produce L- or D-arabitol. The metabolic pathways of these yeasts leading from sugars and glycerol to arabitol are presented. Although the number of reports concerning microbial production of arabitol is rather limited, the research on this topic has been growing for the last several years, with researchers looking for new micro-organisms, substrates and technologies.

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64 citations

Cites background from "Production of ethanol and arabitol ..."

...Currently, arabitol is produced on an industrial scale by the chemical reduction in lactones of arabinonic and lyxonic acids, a reaction that requires an expensive catalyst and a constant temperature of 100°C (Kumdam et al. 2013)....
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...Its efficacy is similar to that of soluble dietary fibres (Mingguo et al. 2011; Kumdam et al. 2013)....
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...Ethanol was a second product, but only a very small amount of that (2 43 g l 1) was detected in the broth and the yield obtained was 0 03 g g 1 (Kumdam et al. 2013)....
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...Its efficacy is similar to that of soluble dietary fibres (Mingguo et al. 2011; Kumdam et al. 2013)....
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...Ethanol was a second product, but only a very small amount of that (2 43 g l (1)) was detected in the broth and the yield obtained was 0 03 g g 1 (Kumdam et al. 2013)....
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Journal Article•DOI•

Artificial neural network and regression coupled genetic algorithm to optimize parameters for enhanced xylitol production by Debaryomyces nepalensis in bioreactor

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Sharon Mano J. Pappu¹, Sathyanarayana N. Gummadi¹•Institutions (1)

Indian Institute of Technology Madras¹

15 Apr 2017-Biochemical Engineering Journal

TL;DR: In this paper, a multi-layer feed forward neural network (ANN) of 5-10-2 topology has been developed to predict the xylitol production and genetic algorithm (GA) was used to find the optimum parameters to enhance xylanol production.

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61 citations

Journal Article•DOI•

Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel-derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid.

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Rosanina Filippousi¹, Dimitrios Antoniou¹, Paschalitsa Tryfinopoulou¹, Aspasia Nisiotou, George-John E. Nychas¹, Apostolis A. Koutinas¹, Seraphim Papanikolaou¹ - Show less +3 more•Institutions (1)

Agricultural University of Athens¹

01 Oct 2019-Journal of Applied Microbiology

TL;DR: To assess the ability of various newly isolated or belonging yeast strains to convert biodiesel‐derived glycerol (Gly) into added‐value compounds, yeast strains from official collections are tested.

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Abstract: Aims To assess the ability of various newly isolated or belonging in official collections yeast strains to convert biodiesel-derived glycerol (Gly) into added-value compounds. Methods and results Ten newly isolated yeast strains belonging to Debaryomyces sp., Naganishia uzbekistanensis, Rhodotorula sp. and Yarrowia lipolytica, isolated from fishes, metabolized Gly under nitrogen limitation. The aim of the study was to identify potential newly isolated microbial candidates that could produce single-cell oil (SCO), endopolysaccharides and polyols when these micro-organisms were grown on biodiesel-derived Gly. As controls producing SCO and endopolysaccharides were the strains Rhodotorula glutinis NRRL YB-252 and Cryptococcus curvatus NRRL Y-1511. At initial Gly (Gly0 ) ≈40 g l-1 , most strains presented remarkable dry cell weight (DCW) production, whereas Y. lipolytica and Debaryomyces sp. produced non-negligible quantities of mannitol and arabitol (Ara). Five strains were further cultivated at increasing Gly0 concentrations. Rhodotorula glutinis NRRL YB-252 produced 7·2 g l-1 of lipid (lipid in DCW value ≈38% w/w), whereas Debaryomyces sp. FMCC Y69 in batch-bioreactor experiment with Gly0 ≈80 g l-1 , produced 30-33 g l-1 of DCW and ~30 g l-1 of Ara. At shake-flasks with Gly0 ≈125 g l-1 , Ara of ~48 g l-1 (conversion yield of polyol on Gly consumed ≈0·62 g g-1 ) was achieved. Cellular lipids of all yeasts contained in variable concentrations oleic, palmitic, stearic and linoleic acids. Conclusions Newly isolated, food-derived and non-previously studied yeast isolates converted biodiesel-derived Gly into several added-value metabolites. Significance and impact of the study Alternative ways of crude Gly valorization through yeast fermentations were provided and added-value compounds were synthesized.

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37 citations

Cites result from "Production of ethanol and arabitol ..."

...Arabitol production achieved compares favorably with most of the literature reports presented in the literature concerning Ara production by yeasts (Kordowska-Wiater et al. 2008; Kordowska-Wiater et al. 2017; Koganti et al. 2011; Koganti and Ju 2013; Kumdam et al. 2013; Kordowska-Wiater 2015)....
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...…of fermentation) demonstrated maximum Ara concentrations lower or in the same magnitude with the results achieved in the current investigation; i.e. Kumdam et al. (2013) determined that Ara was produced from L-arabinose as the sole carbon source at a concentration of 22 7 g l 1 and a yield of 0…...
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Journal Article•DOI•

Production of arabitol from enzymatic hydrolysate of soybean flour by Debaryomyces hansenii fermentation

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Abdullah Al Loman¹, S. M. M. Islam¹, Lu-Kwang Ju¹•Institutions (1)

University of Akron¹

01 Jan 2018-Applied Microbiology and Biotechnology

TL;DR: Arabitol yield increased with increasing C/N ratio and a high I/O-N, suggesting higher yield at stationary phase of low pH, and catabolite repression was observed, with the following order of consumption: glucose > fructose > galactose > xylose > arabinose.

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Abstract: Arabitol is a low-calorie sugar alcohol with anti-cariogenic properties. Enzymatic hydrolysate of soybean flour is a new renewable biorefinery feedstock containing hexose, pentose, and organic nitrogen sources. Arabitol production by Debaryomyces hansenii using soybean flour hydrolysate was investigated. Effects of medium composition, operating conditions, and culture stage (growing or stationary phase) were studied. Production was also compared at different culture volumes to understand the effect of dissolved oxygen concentration (DO). Main factors examined for medium composition effects were the carbon to nitrogen concentration ratio (C/N), inorganic (ammonium) to organic nitrogen ratio (I/O-N), and sugar composition. Arabitol yield increased with increasing C/N ratio and a high I/O-N (0.8–1.0), suggesting higher yield at stationary phase of low pH (3.5–4.5). Catabolite repression was observed, with the following order of consumption: glucose > fructose > galactose > xylose > arabinose. Arabitol production also favored hexoses and, among hexoses, glucose. DO condition was of critical importance to arabitol production and cell metabolism. The yeast consumed pentoses (xylose and arabinose) only at more favorable DO conditions. Finally, arabitol was produced in fermentors using mixed hydrolysates of soy flour and hulls. The process gave an arabitol yield of 54%, volumetric productivity of 0.90 g/L-h, and specific productivity of 0.031 g/g-h.

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27 citations

Journal Article•DOI•

Enhanced d-arabitol production by Zygosaccharomyces rouxii JM-C46: isolation of strains and process of repeated-batch fermentation

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Xianghui Qi¹, Yan Luo¹, Xu Wang¹, Jingfei Zhu¹, Jing Lin¹, Huanhuan Zhang¹, Fang Chen¹, Wenjing Sun¹ - Show less +4 more•Institutions (1)

Jiangsu University¹

25 Feb 2015-Journal of Industrial Microbiology & Biotechnology

TL;DR: A new strain producing high yield of d-arabitol was isolated from hyperosmotic environments and the ITS rDNA sequencing analysis revealed it as Zygosaccharomyces rouxii JM-C46, which has potential to be used for d-Arabitol and xylitol production from glucose via d-ARabitol route.

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Abstract: A new strain producing high yield of d-arabitol was isolated from hyperosmotic environments and the ITS rDNA sequencing analysis revealed it as Zygosaccharomyces rouxii. In addition, using a pH control and repeated-batch fermentation strategy in a 5-L reactor, the maximum yield and the highest volumetric productivity of d-arabitol were 93.48 ± 2.79 g/L and 1.143 g/L h, respectively. Volumetric productivity was successfully improved from 0.86 to 1.143 g/L h, which was increased by 32.9 % after 72 h of fermentation. Z. rouxii JM-C46 has potential to be used for d-arabitol and xylitol production from glucose via d-arabitol route.

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24 citations

Cites background from "Production of ethanol and arabitol ..."

...The production of d-arabitol from d-glucose using osmophilic yeasts belonging to the genera Saccharomycopsis [19], Candida [14], Debaryomyces [9], Pichia [3], Hansenula [16], Metschnikowia [12], and Zygosaccharomyces [5] has been reported....
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Journal Article•DOI•

Fermentation of d -glucose and d -xylose mixtures by Candida tropicalis NBRC 0618 for xylitol production

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Sebastián Sánchez¹, Vicente Bravo², Juan F. García¹, Nicolás Cruz¹, Manuel Cuevas¹ - Show less +1 more•Institutions (2)

University of Jaén¹, University of Granada²

01 May 2008-World Journal of Microbiology & Biotechnology

TL;DR: The fermentation of d-glucose and d-xylose mixtures by the yeast Candida tropicalis NBRC 0618 has been studied under the most favourable operation conditions for the culture, determining the most adequate initial proportion in these sugars for xylitol production.

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Abstract: The fermentation of d-glucose and d-xylose mixtures by the yeast Candida tropicalis NBRC 0618 has been studied under the most favourable operation conditions for the culture, determining the most adequate initial proportion in these sugars for xylitol production. In all the experiments a synthetic culture medium was used, with an initial total substrate concentration of 25 g L−1, a constant pH of 5.0 and a temperature of 30 °C. From the experimental results, it was deduced that the highest values of specific rates of production and of overall yield in xylitol were achieved for the mixtures with the highest percentage of d-xylose, specifically in the culture with the initial d-glucose and d-xylose concentrations of 1 and 24 g L−1, respectively, with an overall xylitol yield of 0.28 g g−1. In addition, the specific rates of xylitol production declined over the time course of the culture and the formation of this bioproduct was favoured by the presence of small quantities of d-glucose. The sum of the overall yield values in xylitol and ethanol for all the experiments ranged from 0.26 to 0.56 g bioproduct/g total substrate.

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23 citations

"Production of ethanol and arabitol ..." refers background in this paper

...Yeast strains such as Candida tropicalis, Pichia stipitis and Pachysolen tannophilus were reported to ferment glucose/xylose mixtures to ethanol and xylitol (Hahn-Hägerdal et al. 2007; Sanchez et al. 2002; Sanchez et al. 2008)....
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Journal Article•

Growth kinetics and ethanol productivity of Saccharomyces cerevisiae PTCC 24860 on various carbon sources.

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H. Shafaghat, Ghasem D. Najafpour, P. S. Rezaei, M. Sharifzadeh

01 Jan 2009-World applied sciences journal

21 citations

"Production of ethanol and arabitol ..." refers background or result in this paper

...The fermentation performance of the organism was found to be better when fructose was used as carbon source (Shafaghat et al. 2009)....
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...Shafaghat et al. (2009) have studied the effect of glucose, fructose and sucrose on the growth and ethanol productivity of Saccharomyces cerevisiae PTCC 24860 and observed similar results....
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Journal Article•DOI•

Effect of pH on growth and ethanol production by Zymomonas

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Hugh G. Lawford¹, Paul Holloway¹, Antonio Ruggiero¹•Institutions (1)

University of Toronto¹

01 Nov 1988-Biotechnology Letters

TL;DR: In this article, the pH range producing the fastest growth of Z. mobilis was 5.5-6.5 with an apparent optimum at 6.5, and the specific rates of glucose utilization and ethanol production were relatively unaffected by pH over the range 7.0-5.5 but increased sharply as the pH was further decreased below 5.0.

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Abstract: In a mineral salts medium containing yeast extract, NH4Cl and glucose (50g/L), the pH range producing the fastest growth ofZ. mobilis was 5.5–6.5 with an apparent optimum at 6.5. At constant growth rate of 0.15hr−1, the specific rates of glucose utilization (qs) and ethanol production (qp) were relatively unaffected by pH over the range 7.0–5.5 but increased sharply as the pH was further decreased below 5.5 to 4.0. Under these conditions the ethanol yield was unaffected by pH over the range 4.0–6.5 but decreased markedly at pH of 7.

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19 citations

"Production of ethanol and arabitol ..." refers background in this paper

...A similar pH range of 4–6 was shown to be optimal for ethanol production by Kluyveromyces marxianus (Bajpai and Margaritis 1987) and Zymomonas mobilis (Lawford et al. 1988)....
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Journal Article•DOI•

Effect of manganese, copper and zinc on the growth of yeast

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J. S. McHargue, R. K. Calfee

01 Jul 1931-Plant Physiology

18 citations

"Production of ethanol and arabitol ..." refers background or methods in this paper

...Online J Biol Sci 1:1005–1008 Azenha M, Teresa M, Vasconcelos SD, Ferreira PM (2000) The Influence of Cu concentration on ethanolic fermentation by Saccharomyces cerevisiae....
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...World J Microbiol Biotechnol 24:709–716 Shafaghat H, Najafpour GD, Rezaei PS, Sharifzadeh M (2009) Growth kinetics and ethanol productivity of Saccharomyces cerevisiae PTCC 24860 on various carbon sources....
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...J Chem Technol Biotechnol 86:217–222 Vesna ST, Vlatka GZ, Damir S, Slobodan G, Nada V (2004) Zinc, copper and manganese enrichment in yeast Saccharomyces cerevisae....
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...Bioresource Technol 96:673–686 Morant JW, Witter LD (1979) Effect of sugars on D-arabitol production and glucose metabolism in Saccharomyces rouxii....
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...Similarly Zn, Fe and Mn were also shown to effect metabolism and by product formation in many yeasts and bacteria (Azenha et al. 2000; McHargue and Calfee 1931; Reeslev and Jensen 1995; Vesna et al. 2004; Fitzpatrick et al. 2001)....
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