Journal ArticleDOI
Production of Lignocellulolytic Enzymes by Microorganisms Isolated from Bulbitermes sp. Termite Gut in Solid-State Fermentation
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TLDR
The findings suggested that saw dust can be used as a cheap renewable raw material for production of lignocellulolytic enzymes and that the fungal-bacterial co-culture could be a good alternative for the enzymes production.Abstract:
Significant amounts of lignocellulosic waste biomass are produced annually. Lignocellulose degrading enzymes play a crucial role in converting the woody materials into sugars and biofuels. The aims of this work were to isolate and characterise the microorganisms from Bulbitermes sp. termite gut with the ability to produce lignocellulolytic enzymes by using forest residues of saw dust as substrate in solid-state fermentation system. Four of the microorganisms (A1, B1, B2, and Br3) with highest lignocellulolytic enzyme activity, which identified as Aspergillus sp., Bacillus sp., Bacillus sp. and Brevibacillus sp., respectively, were selected for characterization. Aspergillus sp. A1 showed highest activities of lignin peroxidase (729.12 U/g) and β-glucosidase (22.97 U/g). Highest activity of endoglucanase (138.77 U/g) and manganese peroxidase (47.73 U/g) were observed in Bacillus sp. B1. The Bacillus sp. B2 produced highest activities of exoglucanase (32.16 U/g) and laccase (71.18 U/g). The highest xylanase (104.96 U/g) activity was observed in Brevibacillus sp. Br3. The production of enzymes particularly for endoglucanase, β-glucosidase, xylanase, lignin peroxidase and laccase were approximate 17–93 % higher in co-culture compared to single culture. The findings suggested that saw dust can be used as a cheap renewable raw material for production of lignocellulolytic enzymes. The present study also indicates that the fungal-bacterial co-culture could be a good alternative for the enzymes production.read more
Citations
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Journal ArticleDOI
Biological Pretreatment of Lignocellulosic Biomass for Biofuels and Bioproducts: An Overview
TL;DR: The selection of highly promising bacterial and/or fungal consortium has the ability to produce various extracellular enzymes including cellulase, hemicellulase, and lignases which can be used in CBP for efficient biological pretreatment of lignocellulosic biomass following production of biofuels and bioproducts.
Journal ArticleDOI
Screening and construction of a novel microbial consortium SSA-6 enriched from the gut symbionts of wood-feeding termite, Coptotermes formosanus and its biomass-based biorefineries
TL;DR: The synergistic interaction of SSA-6 candidates led to significant higher cellulolytic, xylanolytic and ligninolytic activities than that produced by either strain alone ( P = 0.003, 0.003 and 0.004, respectively).
Journal ArticleDOI
Symbiotic cellulolytic bacteria from the gut of the subterranean termite Psammotermes hypostoma Desneux and their role in cellulose digestion
TL;DR: In this study, 33 different bacterial isolates were obtained from the gut of the termite P. hypostoma which were collected using cellulose traps and identified as Paenibacillus lactis, Lysinibacillin macrolides, Stenotrophomonas maltophilia, Lys inibacilli fusiformis and Bacillus cereus, that deposited in GenBank with accession numbers MG991563.
Book ChapterDOI
It Is the Mix that Matters: Substrate-Specific Enzyme Production from Filamentous Fungi and Bacteria Through Solid-State Fermentation.
TL;DR: This review gives an overview of various technically relevant enzymes produced by filamentous fungi and suitable substrates for the production of the enzymes by solid-state fermentation.
Journal ArticleDOI
Fungal-Assisted Valorization of Raw Oil Palm Leaves for Production of Cellulase and Xylanase in Solid State Fermentation Media
Uchenna R. Ezeilo,Roswanira Abdul Wahab,Lee Chew Tin,Iffah Izzati Zakaria,Fahrul Huyop,Naji Arafat Mahat +5 more
TL;DR: The approach adopted by this study offers an alternative avenue to valorizing agriculture biomass, in conjunction to sustainably produce cellulose-acting enzymes to catalyse biofuel and platform chemical productions, yielding satisfactory high titers of cellulase and xylanase.
References
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Journal ArticleDOI
MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods
Koichiro Tamura,Daniel S. Peterson,Nicholas Peterson,Glen Stecher,Masatoshi Nei,Sudhir Kumar +5 more
TL;DR: The newest addition in MEGA5 is a collection of maximum likelihood (ML) analyses for inferring evolutionary trees, selecting best-fit substitution models, inferring ancestral states and sequences, and estimating evolutionary rates site-by-site.
Journal ArticleDOI
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Journal ArticleDOI
16S ribosomal DNA amplification for phylogenetic study.
TL;DR: A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described in this paper.
Journal ArticleDOI
Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species.
Ok-Sun Kim,Yong-Joon Cho,Kihyun Lee,Seok Hwan Yoon,Mincheol Kim,Hyunsoo Na,Sang-Cheol Park,Yoon-Seong Jeon,Jae-Hak Lee,Hana Yi,Sungho Won,Jongsik Chun +11 more
TL;DR: It is concluded that the EzTaxon-e database provides a useful taxonomic backbone for the identification of cultured and uncultured prokaryotes and offers a valuable means of communication among microbiologists who routinely encounter taxonomically novel isolates.
Book
Solid-state fermentation
TL;DR: Solid-state fermentation has emerged as a potential technology for the production of microbial products such as feed, fuel, food, industrial chemicals and pharmaceutical products and with continuity in current trends, SSF technology would be well developed at par with submerged fermentation technology in times to come.