Prognostic Implication of Ezrin Overexpression in Myxofibrosarcomas
Citations
122 citations
Cites methods from "Prognostic Implication of Ezrin Ove..."
...Immunohistochemistry Tissue microarray (TMA) sections were prepared for antigen retrieval as described (4, 26, 28), encompassing all cases subjected tomethylation-specific PCR....
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...The criteria for the diagnosis and parameter assessment were previously elaborated (1, 4, 26)....
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56 citations
55 citations
Cites background from "Prognostic Implication of Ezrin Ove..."
...In fact, ezrin predicted decreasedmetastasis-free survival [86]....
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...Ezrin overexpression in myxofibrosarcomawas associated with a higher histological grade of pathology and cancer stage [86]....
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47 citations
45 citations
References
1,347 citations
"Prognostic Implication of Ezrin Ove..." refers background in this paper
...As a member of the ezrin-radixin-moesin (ERM) protein family, ezrin functions as a linker between the plasma membrane and cortical actin cytoskeleton.(4) Similar to other Society of Surgical Oncology 2010...
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...However, to modulate cytoskeletal rearrangements, the Thr(567) residue at the C-terminal domain of ezrin must be activated by phosphorylation to convert ezrin from a dormant cytoplasmic form to a membrane- and actin-binding conformation.(4) Accordingly, we further compared the endogenous expression and activation status of ezrin protein in myxofibrosarcoma cell lines versus KELFIB fibroblasts....
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...Ezrin functions to integrate various oncogenic signals for cytoskeletal rearrangements via interaction with multiple classes of transmembrane receptors, including CD44, intergrins, and various receptor tyrosine kinases.(4,6,11,22) Among the last, epidermal growth factor receptor and cMet are known to induce the N-terminal tyrosine phosphorylation of ezrin to promote cytoskeletal reorganization and consequent morphogenetic alterations....
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...Through modulation of diverse actin-based functions, ezrin is known as a key component of the molecular chain connecting metastasis-associated cell surface proteins to the signal transduction network.(4,6,21) Despite variation in intensity, phospho-ezrin at Thr(567), the hallmark of ezrin activation, was only detected in myxofibrosarcoma cell 0....
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734 citations
"Prognostic Implication of Ezrin Ove..." refers methods in this paper
...Histological grading and staging were classified according to the updated French Federation of Cancer Centers (FNCLCC) scheme and the American Joint Committee on Cancer (AJCC) system, 6th edition, respectively.(14,15) Retrospective clinical data collection and tissue procurement were in accordance with the local guidelines of research ethics (IRB 99-0506B and 099-04-005)....
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689 citations
"Prognostic Implication of Ezrin Ove..." refers background in this paper
...Among the latter, ezrin overexpression has been recently shown to promote dissemination of pediatric rhabdomyosarcomas and osteosarcomas.(9,10) Its underlying mechanistic basis may be ascribed to increased activated ezrin-implicating signaling programs to modulate pleiotropic cellular phenotypes related to cancerous states, such as substrate adhesion, cell survival, cell migration, and formation of cell–cell junctions....
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...Although the requirement for ezrin in cancer cells to enable successful metastasis seems clear, the regulatory mechanism underlying its overexpression remains less understood.(6,9,10) In rhabdomyosarcomas, ezrin was suggested to be a downstream target of Six-1 homeobox gene, which transcriptionally upregulates ezrin expression to promote metastatic progression....
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511 citations
"Prognostic Implication of Ezrin Ove..." refers background or result in this paper
...Among the last, epidermal growth factor receptor and cMet are known to induce the N-terminal tyrosine phosphorylation of ezrin to promote cytoskeletal reorganization and consequent morphogenetic alterations.(10,23) To date, there is a lack of data on the upstream mechanisms of ezrin activation in myxofibrosarcomas, while our unpublished observation indicated that c-Met protein overexpression, accompanied by mRNA upregulation, is a frequent event in this sarcoma entity....
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...Among the latter, ezrin overexpression has been recently shown to promote dissemination of pediatric rhabdomyosarcomas and osteosarcomas.(9,10) Its underlying mechanistic basis may be ascribed to increased activated ezrin-implicating signaling programs to modulate pleiotropic cellular phenotypes related to cancerous states, such as substrate adhesion, cell survival, cell migration, and formation of cell–cell junctions....
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...In rhabdomyosarcomas, ezrin was suggested to be a downstream target of Six-1 homeobox gene, which transcriptionally upregulates ezrin expression to promote metastatic progression.(10) This was in keeping with our results of real-time PCR quantification and Western blot testing, showing concordance in expression levels of total ezrin between mRNA and protein....
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...Although the requirement for ezrin in cancer cells to enable successful metastasis seems clear, the regulatory mechanism underlying its overexpression remains less understood.(6,9,10) In rhabdomyosarcomas, ezrin was suggested to be a downstream target of Six-1 homeobox gene, which transcriptionally upregulates ezrin expression to promote metastatic progression....
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