scispace - formally typeset
Journal ArticleDOI: 10.1021/ACS.ANALCHEM.0C04997

Promoted "Click" SERS Detection for Precise Intracellular Imaging of Caspase-3.

04 Mar 2021-Analytical Chemistry (American Chemical Society (ACS))-Vol. 93, Iss: 11, pp 4876-4883
Abstract: Although homogeneous detection of some biomolecules has been of great significance in clinical assay, it faces great challenges in achieving precise in situ imaging of biomolecules. In addition, nonspecific adsorption between probes and biomolecules and low sensitivity are still unfathomed problems. Herein, we developed a promoted "Click" surface enhanced Raman scattering (SERS) strategy for realizing highly selective homogeneous detection of biomolecules by simultaneous dual enhanced SERS emissions, obtaining mutually confirmed logical judgment. Taking caspase-3 as one of the biotargets, we have realized highly selective homogeneous detection of caspase-3 using this strategy, and precise intracellular imaging of caspase-3 can be in situ monitored in living cells or during cell apoptosis. In detail, polyA-DNA and the Asp-Glu-Val-Asp (DEVD)-containing peptide sequence were modified into alkyne and nitrile-coded Au nanoparticles (NPs). During the cell apoptosis process, the generated caspase-3 would lead to the cleavage of the tetra-peptide sequence DEVD, thereby removing the negative protection part from the peptide on Au NPs. Interestingly, two different triple bond-labeled Au NPs can be connected together through DNA hybridization to form SERS "hotspot", resulting in simultaneously enlarged triple bond Raman signals. Moreover, we found that the SERS intensity was positively related with caspase-3 concentration, which has a wide linear range (0.1 ng/mL to 10 μg/mL) and low detection limit (7.18 × 10-2 ng/mL). Remarkably, these simultaneously enlarged signals by "Click" SERS could be used for more precise imaging of caspase-3, providing mutually confirmed logical judgment based on two spliced SERS emissions, especially for their relative intensity.

... read more

Citations
  More

7 results found


Open accessJournal ArticleDOI: 10.1063/5.0047578
Li Lin1, Xinyuan Bi1, Yuqing Gu1, Fu Wang1  +1 moreInstitutions (1)
Abstract: Surface-enhanced Raman scattering (SERS) technique has shown extraordinary features for biomedical applications. The implementation of SERS nanotags has opened a new era for bioimaging and detections. As a powerful tool, SERS nanotags provide favorable properties such as fingerprint spectrum, narrow peak linewidth, good photostability, and high spatial resolution accompanied by various rational designs of nanoparticles. They have proven as useful imaging agents for in vivo, ex vivo, and in vitro detection of cancerous cells and tissues. This tutorial provides the basic principles of SERS and SERS nanotags, including recent progress of SERS-based bioimaging applications, as well as the outlooks into the future developments toward practical clinical SERS.

... read more

4 Citations


Open accessJournal ArticleDOI: 10.1039/D1NR00708D
15 Jul 2021-Nanoscale
Abstract: In recent years, bioanalytical surface-enhanced Raman spectroscopy (SERS) has blossomed into a fast-growing research area. Owing to its high sensitivity and outstanding multiplexing ability, SERS is an effective analytical technique that has excellent potential in bioanalysis and diagnosis, as demonstrated by its increasing applications in vivo. SERS allows the rapid detection of molecular species based on direct and indirect strategies. Because it benefits from the tunable surface properties of nanostructures, it finds a broad range of applications with clinical relevance, such as biological sensing, drug delivery and live cell imaging assays. Of particular interest are early-stage-cancer detection and the fast detection of pathogens. Here, we present a comprehensive survey of SERS-based assays, from basic considerations to bioanalytical applications. Our main focus is on SERS-based pathogen detection methods as point-of-care solutions for early bacterial infection detection and chronic disease diagnosis. Additionally, various promising in vivo applications of SERS are surveyed. Furthermore, we provide a brief outlook of recent endeavours and we discuss future prospects and limitations for SERS, as a reliable approach for rapid and sensitive bioanalysis and diagnosis.

... read more

Topics: Bioanalysis (52%)

3 Citations


Journal ArticleDOI: 10.1016/J.BIOS.2021.113539
Qiao-Yan Jiang1, Ximin Cui2, Yang Sun1, Zhengsheng Mao1  +4 moreInstitutions (2)
Abstract: Caspase-3 and hydrogen peroxide (H2O2) are closely associated with numerous diseases, both of them are vital in different physiological and pathological conditions. They are closely related and also can act independently. The selective and accurate determination of caspase-3 and H2O2 simultaneously to determine their state of being in different situations is of great significance for further study of their molecular mechanisms and the elucidation of their biological functions. In our latest research, a AuNPL-crown nanoprobe was obtained by attaching (4-aminosulfonylphenyl) boronic acid (4-APBA) and peptide-FITC (NH2-Asp-Glu-Val-Asp (DEVD)-FITC) to gold nanoplates (AuNPLs). The fabricated AuNPL-crown nanoprobe was used for dual-channel and real-time tracking of the dynamic changes in caspase-3 and H2O2 based on fluorescence resonance energy transfer (FRET)/surface-enhanced Raman spectroscopy (SERS) technology. The AuNPL-crown nanoprobe not only provides synergy but can also achieve noninterference, making the results more reliable and repeatable. This study simultaneously traced the dynamic changes of caspase-3 and H2O2 on a single probe, which provides a potential new platform for the analysis of caspase-3 and H2O2 in the biological environment with high accuracy, sensitivity, convenience, and efficiency. In summary, we develop a new strategy for the simultaneous detection of different substances on a single probe.

... read more

Topics: Nanoprobe (59%)

Journal ArticleDOI: 10.1021/ACS.ANALCHEM.1C02158
Bo Hu1, Qin Zhang1, Xiaonan Gao1, Kehua Xu1  +1 moreInstitutions (1)
Abstract: Pyroptosis is closely related to inhibiting the occurrence and development of tumors. However, the pyroptosis pathways (PPs) impacted by different stimulants are still unknown. To accurately understand the PP in cancer cells, we designed a multicolor fluorescent nanoprobe (Cas-NP) to monitor the activation of caspases-1/3/4 during pyroptosis. The Cas-NP was prepared by the assembly of three different fluorophores-labeled peptides, specific response to caspases-1/3/4 on Au nanoparticles via the Au-Se bond to in situ monitor caspase-1/3/4 with high selectivity and sensitivity. Moreover, the selenopeptide specific to caspase-4 (Cyanine-5-LEVD-SeH) was synthesized for the first time to overcome the difficulty in commercial synthesis. During the pyroptosis of cancer cells induced by adenosine triphosphate (ATP), only the fluorescence of caspase-1 significantly increases. When the cells are stimulated with lipopolysaccharide (LPS), the fluorescence signals corresponding to caspases-3 and 4 first appear and then the fluorescence of caspase-1 is observed. Furthermore, the inhibitor study indicates that the activated caspase-4 can lead to the activation of caspase-1 after the LPS treatment. We first discovered that caspase-3 is activated during the pyroptosis process stimulated by LPS and further verified the activation sequence of caspases-1/3/4 via visualized fluorescence detection. The study provides an effective tool for understanding complex signaling mechanisms in pyroptosis cells and new ideas to explore useful therapeutic inhibitors based on pyroptosis.

... read more

Topics: Pyroptosis (69%)

Journal ArticleDOI: 10.1016/J.MICROC.2021.106787
Guo-Xi Liang1, Kai-Ren Zhao1, Yu-Sheng He1, Zhi-Jun Liu1  +2 moreInstitutions (1)
Abstract: Synthesis of highly efficient, cost-effective, Easy labeling electrochemiluminescence (ECL) luminescent materials is attractive in developing highly sensitive ECL sensors for many applications such as clinical diagnostics, biosecurity, and environmental monitoring. Herein, by doping carbon dots (CDots) and gold nanoparticles (AuNPs) into zeolitic imidazolate framework (ZIF-8), novel high-efficiency ECL emitters CDots@ZIF-8/AuNPs nanocomposites were prepared. Our experiment indicated that ZIF-8 in the nanocomposites not only act as carrier for ECL labels, but as an ECL accelerator to convert the CDots to CDots•–, causing the enhanced ECL intensity of CDots in K2S2O8 solution. AuNPs, which have excellent conductivity, were formed in the pores of ZIF-8 to further enhance the ECL signals. As a result, the nanocomposites showed a final about 7-fold ECL intensity as compared to the pure CDots. AuNPs in the nanocomposites could also provide a platform for effective attaching the biomolecular, making the nanocomposites ideally suited for building biosensor. As a proof of concept, the prepared CDots@ZIF-8/AuNPs was successfully applied in the rapid and simple ECL monitoring of caspase-3 activity during cell apoptosis, which gives impetus to the design of new ECL luminescent materials with ultimate applications in highly sensitive bioanalysis.

... read more

Topics: Electrochemiluminescence (52%)

References
  More

31 results found


Open accessJournal ArticleDOI: 10.1021/ACSNANO.9B04224
28 Jan 2020-ACS Nano
Abstract: The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experimental and theoretical effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technology, but additional efforts are still needed before it can be routinely used analytically and in commercial products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technological development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the preparation of this article.

... read more

754 Citations


Journal ArticleDOI: 10.1038/NATURE22393
Yupeng Wang1, Wenqing Gao1, Xuyan Shi1, Jingjin Ding  +4 moreInstitutions (1)
01 May 2017-Nature
Abstract: Pyroptosis is a form of cell death that is critical for immunity. It can be induced by the canonical caspase-1 inflammasomes or by activation of caspase-4, -5 and -11 by cytosolic lipopolysaccharide. The caspases cleave gasdermin D (GSDMD) in its middle linker to release autoinhibition on its gasdermin-N domain, which executes pyroptosis via its pore-forming activity. GSDMD belongs to a gasdermin family that shares the pore-forming domain. The functions and mechanisms of activation of other gasdermins are unknown. Here we show that GSDME, which was originally identified as DFNA5 (deafness, autosomal dominant 5), can switch caspase-3-mediated apoptosis induced by TNF or chemotherapy drugs to pyroptosis. GSDME was specifically cleaved by caspase-3 in its linker, generating a GSDME-N fragment that perforates membranes and thereby induces pyroptosis. After chemotherapy, cleavage of GSDME by caspase-3 induced pyroptosis in certain GSDME-expressing cancer cells. GSDME was silenced in most cancer cells but expressed in many normal tissues. Human primary cells exhibited GSDME-dependent pyroptosis upon activation of caspase-3 by chemotherapy drugs. Gsdme-/- (also known as Dfna5-/-) mice were protected from chemotherapy-induced tissue damage and weight loss. These findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy.

... read more

Topics: Pyroptosis (69%), Caspase 1 (68%), Caspase (51%)

660 Citations


Journal ArticleDOI: 10.1146/ANNUREV.PHYSIOL.010908.163111
Abstract: Cell death was once viewed as unregulated. It is now clear that at least a portion of cell death is a regulated cell suicide process. This type of death can exhibit multiple morphologies. One of these, apoptosis, has long been recognized to be actively mediated, and many of its underlying mechanisms have been elucidated. Moreover, necrosis, the traditional example of unregulated cell death, is also regulated in some instances. Autophagy is usually a survival mechanism but can occur in association with cell death. Little is known, however, about how autophagic cells die. Apoptosis, necrosis, and autophagy occur in cardiac myocytes during myocardial infarction, ischemia/reperfusion, and heart failure. Pharmacological and genetic inhibition of apoptosis and necrosis lessens infarct size and improves cardiac function in these disorders. The roles of autophagy in ischemia/reperfusion and heart failure are unresolved. A better understanding of these processes and their interrelationships may allow for the development of novel therapies for the major heart syndromes.

... read more

Topics: Programmed cell death (60%), Autophagy (56%), Necrosis (53%) ... read more

603 Citations


Journal ArticleDOI: 10.1021/ACS.CHEMREV.7B00668
Cheng Zong1, Mengxi Xu1, Li-Jia Xu1, Ting Wei1  +4 moreInstitutions (1)
11 Apr 2018-Chemical Reviews
Abstract: Surface-enhanced Raman spectroscopy (SERS) inherits the rich chemical fingerprint information on Raman spectroscopy and gains sensitivity by plasmon-enhanced excitation and scattering. In particular, most Raman peaks have a narrow width suitable for multiplex analysis, and the measurements can be conveniently made under ambient and aqueous conditions. These merits make SERS a very promising technique for studying complex biological systems, and SERS has attracted increasing interest in biorelated analysis. However, there are still great challenges that need to be addressed until it can be widely accepted by the biorelated communities, answer interesting biological questions, and solve fatal clinical problems. SERS applications in bioanalysis involve the complex interactions of plasmonic nanomaterials with biological systems and their environments. The reliability becomes the key issue of bioanalytical SERS in order to extract meaningful information from SERS data. This review provides a comprehensive over...

... read more

603 Citations


Journal ArticleDOI: 10.1039/C2CP42598J
Abstract: In this perspective we discuss the roles of hot spots in surface-enhanced Raman spectroscopy (SERS). After giving background and defining the hot spot, we evaluate a variety of SERS substrates which often contain hot spots. We compare and discuss the differentiating properties of each substrate. We then provide a thorough analysis of the hot spot contribution to the observed SERS signal both in ensemble-averaged and single-molecule conditions. We also enumerate rules for determining the SERS enhancement factor (EF) to clarify the use of this common metric. Finally, we present a forward-looking overview of applications and uses of hot spots for controlling chemistry on the nanoscale. Although not exhaustive, this perspective is a review of some of the most interesting and promising methodologies for creating, controlling, and using hot spots for electromagnetic amplification.

... read more

532 Citations