Propagation of eight cultiv ars of Rhododendron in vitro using agar-solidified and liquid media and direct rooting of shoots in vivo
TL;DR: Sterile cultures were obtained from expanding buds of Rhododendron cultivars ‘Pink Pearl’, ‘Nova Zembla’ and ‘Gomer Waterer”, and shoot production with liquid medium was 10-fold higher than with agarsolidified medium, which increased the number of roots produced per shoot.
About: This article is published in Scientia Horticulturae.The article was published on 1984-12-01. It has received 41 citations till now. The article focuses on the topics: Shoot & Vegetative reproduction.
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01 Jan 2005
TL;DR: The design of bioreactor systems for large scale production of horticultural and medicinal plants and their application in mass propagation of somatic embryos as a compromise between liquid and solidified media are studied.
Abstract: Contributing Authors Preface 1. General introduction: a personal reflection on the use of liquid media for in vitro culture Walter Preil I. Bioreactors 2. Application of bioreactor design principles to plant micropropagation Wayne R. Curtis 3. Bioreactor design for propagation of somatic embryos Anne Kathrine Hvoslef-Eide, Odd Arild S. Olsen, Ragnhild Lyngved, Cristel Munster & Petter H. Heyerdahl 4. Practical aspects of bioreactor application in mass propagation of plants S. Takayama & M. Akita 5. Simple bioreactors for mass propagation of plants Meira Ziv 6. Application of bioreactor systems for large scale production of horticultural and medicinal plants K.Y. Paek, Debasis Chakrabarty & E.J. Hahn 7. Membranes to reduce adherence of somatic embryos to the cell lift impeller of a bioreactor Seppo Sorvari, Riitta Makelainen, Katriina Ahanen & Otto Toldi 8. Cost-effective mass cloning of plants in liquid media using a novel growtek bioreactor Satyahari Dey 9. Multiplication of Chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems Eun-Joo Hahn & Kee-Yoeup Paek 10. Control of growth and differentiation of bioreactor cultures of Physcomitrella by environmental parameters Annette Hohe & Ralf Reski II. Temporary Immersion Systems 11. Temporary immersion system: a new concept for use liquid medium in mass propagation M. Berthouly & H. Etienne 12. Mass propagation of tropical crops in temporary immersion systems Elio Jimenez Gonzalez 13. Use of growth retardants for banana (Musa AAA cv. Grand Naine) shoot multiplication in temporary immersion systems Nilca Albany, Elio Jimenez, Jorge Vilchez, Leyanis Garcia, Manuel De Feria, Naivy Perez, Zoe Sarria, Blanca Perez & Justo Clavelo 14. Somatic embryogermination of Psidium guajava L. in the Rita(R) temporary immersion system and on semisolid medium Rafael Gomez Kosky, J. Vilchez Perozo, N. Albany Valero & D. Agramonte Penalver 15. Application of a temporary immersion system in mass propagation of Phalaenopsis Tino Hempfling & Walter Preil 16. Propagation of Prunus and Malus by temporary immersion C. Damiano, S.R. La Starza, S. Monticelli, A. Gentile, E. Caboni & A. Frattarelli 17. Optimisation of growing conditions for the apple rootstock M26 grown in RITA containers using temporary immersion principle Li-Hua Zhu, Xue-Yuan Li & Margareta Welander 18. Experimental use of a novel temporary immersion system for liquid culture of olive microshoots Katerina Grigoriadou, Miltiadis Vasilakakis, Theofilos Tzoulis & Eleftherios P. Eleftheriou 19. Shoot regeneration from nodules of Charybdis sp.: A comparison of semisolid, liquid and temporary immersion culture systems Ch. Wawrosch, A. Kongbangkerd, A. Kopf & B. Kopp III. Somatic Embryogenesis And Shoot Initiation 20. Propagation of Norway spruce via somatic embryogenesis Sara von Arnold, Peter Bozhkov, David Clapham, Julia Dyachok, Lada Filonova, Karl-Anders Hogberg, Mathieu Ingouff & Malgorzata Wiweger 21. Norway spruce somatic embryogenesis: membrane rafts as a compromise between liquid and solidified media M. Vagner, Z. Vondrakova, L. Fischerova & J. Opatrna 22. Picea abies somatic embryo development from suspension cultures and agar-based cultures: a comparison Christopher Hunter & Lionel Levy 23. Somatic embryogenesis by liquid culture of epidermal layers in sunflower: from genetic control to cell development Michel Petitprez, A. Sarrafi, E. Flores-Berrios, X. Xuhan, C. Briere & L. Gentzbittel 24. Development of photoautotrophy in Coffea somatic embryos enables mass production of clonal transplants F.
136 citations
Cites background from "Propagation of eight cultiv ars of ..."
...For example, in some Rhododendron cultivars, shoot production in liquid medium was 10-fold higher than with agar-gelled medium (Douglas, 1984)....
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TL;DR: Micropropagation in bioreactors for optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of plants in liquid culture systems.
Abstract: The most common methods of micropropagation involve the proliferation of shoots via a semi solid system. While such semi solid systems have been moderately to highly successful in terms of multiplication yields, it has become increasingly important to improve productivity and reduce the time taken to multiply commercially important material. Micropropagation by conventional techniques is typically a labor intensive time taking means of clonal propagation. To overcome this, the use of shake cultures utilizing liquid culture medium has been promoted. The liquid medium allows the close contact with the tissue which stimulates and facilitates the uptake of nutrients and phytohormones, leading to better shoot and root growth. Continuous shaking promotes lesser expression of apical dominance which generally leads to induction and proliferation of numerous axillary buds. Further, with in the shake culture conditions, the growth and multiplication rate of shoots is enhanced by forced aeration, since continuous shaking of medium provides ample oxygen supply to the tissue which ultimately leads to their faster growth. Bioreactor provides a rapid and efficient clonal propagation system utilizing liquid medium to avoid intensive manual handling. Automation of micropropagation in bioreactors has been advanced by several authors as a possible way of reducing cost of micropropagation. Micropropagation in bioreactors for optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of plants in liquid culture systems.
100 citations
Cites background from "Propagation of eight cultiv ars of ..."
...…Development favors the production of large number of plants which are more or less true-to-type (Takayama and Misawa, 1981; Harris and Mason, 1983; Douglas, 1984; Pierik, 1987; Chu et al., 1993) Furthermore, within the shake culture conditions, the growth and multiplication rate of the shoots is…...
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01 Jan 1995
TL;DR: Many tissue culture laboratories still operate with the assumption that vessels, gels, and related variables are minor players in development of new in vitro strategies, in spite of ample evidence that these underrated variables exert considerable leverage on in vitro productivity, and warrant more comprehensive description and interpretation.
Abstract: A deliberate change in the growth regulators added to a tissue culture medium can elicit a dramatic response in a cultured plant. This cause-effect relationship is well-recognized, and as a result, growth regulator treatments are carefully designed based on the anticipated consequences to in vitro performance. On the other hand, decisions concerning selection of a containment vessel, or the physical status (phase) of in vitro media are frequently based on cost, availability, or convenience, without much appreciation for their broader repercussions. In fact, these underestimated variables related to the tissue culture vessel (size, shape, closure) or the medium phase (gelling agents, liquid medium, physical supports) can conspicuously modify in vitro plant behavior, often more predictably and cost-effectively than chemical additives to a medium. The potent physicochemical influences dictated by vessels & gels have frequently been cited (Chu et al. 1993; Fujiwara and Kozai 1994, this volume; Kozai et al. 1992; Obeidy and Smith 1990; McClelland and Smith 1990; Smith and McClelland 1991), paving the way for practical management of these tools to optimize tissue culture systems. Many tissue culture laboratories still operate with the assumption that vessels, gels, and related variables are minor players in development of new in vitro strategies, in spite of ample evidence that these underrated variables exert considerable leverage on in vitro productivity, and warrant more comprehensive description and interpretation.
83 citations
01 Jan 2005
78 citations
Cites background from "Propagation of eight cultiv ars of ..."
...For example, in some Rhododendron cultivars, shoot production in liquid medium was 10-fold higher than with agar-gelled medium ( Douglas, 1984 )....
[...]
TL;DR: This in vitro strategy can be a reliable method for the steady production of a large number of plants for essential oil production, which is reported for the first time for A. vulgaris.
66 citations
References
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TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Abstract: In experiments with tobacco tissue cultured on White's modified medium (basal meditmi hi Tnhles 1 and 2) supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Subse(iueutly it was found Ihiit this jnoniotiou oi' f^rowih was due mainly though nol entirely to inorj^auic rather than organic con.stitttenls in the extract. In the isolation of Rrowth factors from plant tissues and other sources inorj '̂anic salts are fre(|uently carried along with fhe organic fraclioits. When tissue cultures are used for bioassays, therefore, il is necessary lo lake into account increases in growth which may result from nutrient elements or other known constituents of the medium which may he present in the te.st materials. To minimize interference trom rontaminaitis of this type, an altempt has heen made to de\\eh)p a nieditmi with such adequate supplies of all re(iuired tnineral nutrients and cotntnott orgattic cottslitueitls that no apprecial»le change in growth rate or yield will result from the inlroduclion of additional amounts in the range ordinarily expected to be present in tnaterials to be assayed. As a point of referetice for this work some of the culture media in mc)st common current use will he cotisidered briefly. For ease of comparis4)n Iheir mineral compositions are listed in Tables 1 and 2. White's nutrient .solution, designed originally for excised root cultures, was based on Uspeuski and Uspetiskaia's medium for algae and Trelease and Trelease's micronutrieni solution. This medium also was employed successfully in the original cttltivation of callus from the tobacco Iiybrid Nicotiana gtauca x A', tanijadorffii, atitl as further modified by White in 194̂ ^ and by others it has been used for the
63,098 citations
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
9,342 citations
Book•
01 Jan 1968
TL;DR: This book discusses Propagation Methods and Rootstocks for the Important Fruit and Nut Species, and theoretical Aspects of Grafting and Budding, and Principles of Tissue Culture for Micropropagation.
Abstract: PART I. GENERAL ASPECTS OF PROPAGATION. 1. Introduction. 2. Propagation Structures. PART II. 3. The Development of Seeds and Spores. 4. Production of Genetically Pure Seed. 5. Techniques of Seed Production and Handling. 6. Principles of Propagation by Seeds. 7. Techniques of Propagation by Seeds. PART III. 8. Vegetative Propagation. 9. Anatomical and Physiological Basis of Propagation by Cuttings. 10. Techniques of Propagation by Cuttings. 11. Theoretical Aspects of Grafting and Budding. 12. Techniques of Grafting. 13. Techniques of Budding. 14. Layering and Its Natural Modifications. 15. Propagation by Specialized Stems and Roots. 16. Principles of Tissue Culture for Micropropagation. 17. Techniques of In Vitro Micropropagation. PART IV. 18. Propagation Methods and Rootstocks for the Important Fruit and Nut Species. 19. Propagation of Ornamental Trees, Shrubs, and Woody Vines. 20. Propagation of Selected Annuals and Herbaceous Perennials Used as Ornamentals.
2,977 citations
661 citations
TL;DR: The introduction of some additional new techniques may possibly reduce the cost and broaden the range of plants that can be propagated economically in vitro and its adaptation to commercial practices.
310 citations