Properties of Protein Monolayers

The properties of insulin films, spread at the air-water interface

We have studied the haemolysing action of lysocithin on the chromocytes of man, rabbit and sheep. It was found that the amount of lysocithin needed to haemolyse a red cell is sufficient to cover this cell by a monomolecular layer. In view of the fact that lysocithin alters the shape (and therefore the surface) of the chromocytes before haemolysis sets in, no very accurate check is possible.



The lysocithin used was prepared from egg-yolk. In a series of preliminary experiments with lyosocithin prepared from ovolecithin Merck the general features of lysocithin haemolysis were studied. It was shown that the number of chromocytes haemolysed by a given quantity of lysocithin is independent of the concentrations used. And further, that the process of lysocithin haemolysis is terminated within less than 15 minutes, even if insufficient lysocithin is present for the haemolysis to be complete.

On the haemolysing action of lysocithin

A relative rigidity of the monolayer of stearic acid on the surface of water containing various metallic ions was measured by the displacement of the glass thread It was found, as was pointed out in the preceding paper, that metallic ions were classified largely into two groups, namely ions which render the surface film remarkably rigid and those without such effect Those of the former group are thorium, aluminum, iron, copper, zinc, mercury, cobalt and nickel, while the latter group involves calcium, barium and magnesium So-called expansion pattern of the film of stearic acid in the presence of these metallic ions was also studied, the result of which also confirmed this conclusion

Studies on Monomolecular Films. VIII. Rigidity of the Monolayer of Stearic Acid on the Surface of Salt Solutions

LITTLE is yet known regarding the change undergone by a native protein when it is adsorbed at an interface. The evidence at present available, which is largely based upon the preservation of the activity of enzymes in monolayers1, would seem to indicate that the molecules are subject to a change of form which remains reversible during the initial period of the adsorption.

Properties of Protein Monolayers

Citations

The properties of insulin films, spread at the air-water interface

On the haemolysing action of lysocithin

Studies on Monomolecular Films. VIII. Rigidity of the Monolayer of Stearic Acid on the Surface of Salt Solutions

A Possible Alternative Orientation in Protein Monolayers

Related Papers (5)

Properties and Structure of Protein Monolayers.

The structure of protein monolayers

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The Ionic Permeability of Adsorbed Membrane Protein Monolayers