Prospective and development of butanol as an advanced biofuel.
TL;DR: Continuous operation is more productive for large scale production of butanol as a biofuel, but a single chemostat bioreactor cannot achieve this goal for the biphasic ABE fermentation, and tanks-in-series systems should be optimized for alternative feedstocks and new strains.
About: This article is published in Biotechnology Advances.The article was published on 2013-12-01. It has received 233 citations till now. The article focuses on the topics: Butanol & Biofuel.
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TL;DR: In this article, the updated progress of ABE fermentation techniques is summarized from the aspects: (i) selection of suitable strain; (ii) availability of cheaper substrates; (iii) development of fermentation engineering; and (iv) the research on ABE combustion in internal combustion engine (ICEs).
213 citations
TL;DR: An overview of recent advances in C. acetobutylicum strain engineering and process development focusing on in situ product recovery is provided and state-of-the-art genome editing tools such as CRISPR-Cas for targeted gene knock-out and knock-in are explored.
196 citations
TL;DR: The latest studies on butanol recovery techniques including gas stripping, liquid–liquid extraction, adsorption, and membrane-based techniques, which can be used for in situ recovery of inhibitory products to enhance butanol production are reviewed.
Abstract: Butanol has recently gained increasing interest due to escalating prices in petroleum fuels and concerns on the energy crisis. However, the butanol production cost with conventional acetone–butanol–ethanol fermentation by Clostridium spp. was higher than that of petrochemical processes due to the low butanol titer, yield, and productivity in bioprocesses. In particular, a low butanol titer usually leads to an extremely high recovery cost. Conventional biobutanol recovery by distillation is an energy-intensive process, which has largely restricted the economic production of biobutanol. This article thus reviews the latest studies on butanol recovery techniques including gas stripping, liquid–liquid extraction, adsorption, and membrane-based techniques, which can be used for in situ recovery of inhibitory products to enhance butanol production. The productivity of the fermentation system is improved efficiently using the in situ recovery technology; however, the recovered butanol titer remains low due to the limitations from each one of these recovery technologies, especially when the feed butanol concentration is lower than 1 % (w/v). Therefore, several innovative multi-stage hybrid processes have been proposed and are discussed in this review. These hybrid processes including two-stage gas stripping and multi-stage pervaporation have high butanol selectivity, considerably higher energy and production efficiency, and should outperform the conventional processes using single separation step or method. The development of these new integrated processes will give a momentum for the sustainable production of industrial biobutanol.
139 citations
Cites methods from "Prospective and development of buta..."
...Hybrid process...
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...Feedstock selection, fermentation process optimization, and strain engineering have been well reviewed (Jang et al. 2012; Lütke-Eversloh and Bahl 2011; Papoutsakis 2008; Xue et al. 2013a; Zhao et al. 2013) and thus will not be covered in this review....
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...1b (Xue et al. 2013b)....
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TL;DR: This gas stripping‐pervaporation process with less contaminated risk is effective in increasing butanol production and reducing energy consumption.
Abstract: Butanol is considered as an advanced biofuel, the development of which is restricted by the intensive energy consumption of product recovery. A novel two-stage gas stripping-pervaporation process integrated with acetone-butanol-ethanol (ABE) fermentation was developed for butanol recovery, with gas stripping as the first-stage and pervaporation as the second-stage using the carbon nanotubes (CNTs) filled polydimethylsiloxane (PDMS) mixed matrix membrane (MMM). Compared to batch fermentation without butanol recovery, more ABE (27.5 g/L acetone, 75.5 g/L butanol, 7.0 g/L ethanol vs. 7.9 g/L acetone, 16.2 g/L butanol, 1.4 g/L ethanol) were produced in the fed-batch fermentation, with a higher butanol productivity (0.34 g/L · h vs. 0.30 g/L · h) due to reduced butanol inhibition by butanol recovery. The first-stage gas stripping produced a condensate containing 155.6 g/L butanol (199.9 g/L ABE), which after phase separation formed an organic phase containing 610.8 g/L butanol (656.1 g/L ABE) and an aqueous phase containing 85.6 g/L butanol (129.7 g/L ABE). Fed with the aqueous phase of the condensate from first-stage gas stripping, the second-stage pervaporation using the CNTs-PDMS MMM produced a condensate containing 441.7 g/L butanol (593.2 g/L ABE), which after mixing with the organic phase from gas stripping gave a highly concentrated product containing 521.3 g/L butanol (622.9 g/L ABE). The outstanding performance of CNTs-PDMS MMM can be attributed to the hydrophobic CNTs giving an alternative route for mass transport through the inner tubes or along the smooth surface of CNTs. This gas stripping-pervaporation process with less contaminated risk is thus effective in increasing butanol production and reducing energy consumption.
136 citations
Cites background or methods from "Prospective and development of buta..."
...In recent study, we also conducted two-stage gas stripping for butanol recovery from ABE fermentation, producing 420.3 g/L butanol (532.2 g/L ABE) in the final product mixture (Xue et al., 2013b)....
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...Butanol is considered an advanced biofuel, which can be produced from renewable resources by acetone-butanol-ethanol (ABE) fermentation usingClostridium spp. (D€urre, 2007; Xue et al., 2013a)....
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01 Jun 2012
TL;DR: A review of the recent trends in systems metabolic engineering for the production of chemicals and materials can be found in this paper, where the authors present general strategies and showcase representative examples of representative examples.
Abstract: Metabolic engineering has contributed significantly to the enhanced production of various value-added and commodity chemicals and materials from renewable resources in the past two decades. Recently, metabolic engineering has been upgraded to the systems level (thus, systems metabolic engineering) by the integrated use of global technologies of systems biology, fine design capabilities of synthetic biology, and rational-random mutagenesis through evolutionary engineering. By systems metabolic engineering, production of natural and unnatural chemicals and materials can be better optimized in a multiplexed way on a genome scale, with reduced time and effort. Here, we review the recent trends in systems metabolic engineering for the production of chemicals and materials by presenting general strategies and showcasing representative examples.
128 citations
References
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TL;DR: The integration of agroenergy crops and biorefinery manufacturing technologies offers the potential for the development of sustainable biopower and biomaterials that will lead to a new manufacturing paradigm.
Abstract: Biomass represents an abundant carbon-neutral renewable resource for the production of bioenergy and biomaterials, and its enhanced use would address several societal needs. Advances in genetics, biotechnology, process chemistry, and engineering are leading to a new manufacturing concept for converting renewable biomass to valuable fuels and products, generally referred to as the biorefinery. The integration of agroenergy crops and biorefinery manufacturing technologies offers the potential for the development of sustainable biopower and biomaterials that will lead to a new manufacturing paradigm.
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TL;DR: Here, the natural resistance of plant cell walls to microbial and enzymatic deconstruction is considered, collectively known as “biomass recalcitrance,” which is largely responsible for the high cost of lignocellulose conversion.
Abstract: Lignocellulosic biomass has long been recognized as a potential sustainable source of mixed sugars for fermentation to biofuels and other biomaterials. Several technologies have been developed during the past 80 years that allow this conversion process to occur, and the clear objective now is to make this process cost-competitive in today's markets. Here, we consider the natural resistance of plant cell walls to microbial and enzymatic deconstruction, collectively known as "biomass recalcitrance." It is this property of plants that is largely responsible for the high cost of lignocellulose conversion. To achieve sustainable energy production, it will be necessary to overcome the chemical and structural properties that have evolved in biomass to prevent its disassembly.
4,035 citations
TL;DR: Histoire-substrats-biochimie and physiologie, facteurs favorisant le passage de the production d'acides a la production de solvents a la phase of production de Solvents, andrology and physiology, et developpement du procede.
1,920 citations
TL;DR: This article reviews biotechnological production of butanol by clostridia and some relevant fermentation and downstream processes and the strategies for strain improvement by metabolic engineering and further requirements to make fermentative butanol production a successful industrial process.
Abstract: Butanol is an aliphatic saturated alcohol having the molecular formula of C4H9OH Butanol can be used as an intermediate in chemical synthesis and as a solvent for a wide variety of chemical and textile industry applications Moreover, butanol has been considered as a potential fuel or fuel additive Biological production of butanol (with acetone and ethanol) was one of the largest industrial fermentation processes early in the 20th century However, fermentative production of butanol had lost its competitiveness by 1960s due to increasing substrate costs and the advent of more efficient petrochemical processes Recently, increasing demand for the use of renewable resources as feedstock for the production of chemicals combined with advances in biotechnology through omics, systems biology, metabolic engineering and innovative process developments is generating a renewed interest in fermentative butanol production This article reviews biotechnological production of butanol by clostridia and some relevant fermentation and downstream processes The strategies for strain improvement by metabolic engineering and further requirements to make fermentative butanol production a successful industrial process are also discussed Biotechnol Bioeng 2008;101: 209-228 © 2008 Wiley Periodicals, Inc
1,017 citations
TL;DR: The best‐studied bacterium to perform a butanol fermentation is Clostridium acetobutylicum, and its genome has been sequenced, and the regulation of solvent formation is under intensive investigation, opening the possibility to engineer recombinant strains with superior biobutanol‐producing ability.
Abstract: Biofuels are an attractive means to prevent a further increase of carbon dioxide emissions. Currently, gasoline is blended with ethanol at various percentages. However, butanol has several advantages over ethanol, such as higher energy content, lower water absorption, better blending ability, and use in conventional combustion engines without modification. Like ethanol, it can be produced fermentatively or petrochemically. Current crude oil prices render the biotechnological process economic again. The best-studied bacterium to perform a butanol fermentation is Clostridium acetobutylicum. Its genome has been sequenced, and the regulation of solvent formation is under intensive investigation. This opens the possibility to engineer recombinant strains with superior biobutanol-producing ability.
905 citations