Journal ArticleDOI
Proteases of the genus Bacillus. I. Neutral proteases
Leonard Keay,Bernard S Wildi +1 more
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The B. subtilis NRRL B3411 neutral protease has been extensively purified by solvent, and salt fractional ion, pigment removal with DEAE‐cellulose followed by chromatography on hydroxylapatite, and a final passage through a Sephadex G‐100 column.Abstract:
B. subtilis NRRL B3411 neutral protease has been extensively purified by solvent, and salt fractional ion, pigment removal with DEAE‐cellulose followed by chromatography on hydroxylapatite, and a final passage through a Sephadex G‐100 column. The neutral protease was shown to be homogeneous by disc gel and cellulose acetate electrophoresis, gel filtration chromatography, and ultra‐centrifugation. The molecular weight was determined by osmometry and ultracentrifugation to be about 38–42,000 and the amino acid composition and zinc content determined. The general properties of the enzyme, pH‐activity relationship, stability, effect of inhibitors, and specificity are discussed. Comparative studies were carried out on the B. subtilis NRRL B3411 and B. subtilis var. amylosacchariticus neutral proteases and these enzymes were found to be indistinguishable by the methods used, but quite distinct from the thermostable enzyme thermolysin from B. thermoprotcolyticus.read more
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Microbial alkaline proteases: From a bioindustrial viewpoint
C. Ganesh Kumar,Hiroshi Takagi +1 more
TL;DR: In this paper, a review of the proteases that can resist extreme alkaline environments produced by a wide range of alkalophilic microorganisms is presented, and various nutritional and environmental parameters affecting the production of alkaline proteases are delineated.
Journal ArticleDOI
Extracellular enzyme synthesis in the genus Bacillus.
TL;DR: This article will attempt to review the recent literature concerned with the characterization and properties of the exoenzymes synthesized by the bacilli and the control and mechanisms of their synthesis.
Journal ArticleDOI
The biology of mycorrhiza in the Ericaceae
V Pearson,David Read +1 more
TL;DR: It was revealed that H. ericae was able to produce significantly higher yield when grown on intact fungal necromass than when provided with equivalent concentrations of N in the form of ammonium.
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Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation
TL;DR: In this article, a comparative study was carried out on the production of neutral protease using agro-industrial residues as substrate in solid-state fermentation (SSF) and submerged fermentation (SmF).
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Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein.
TL;DR: Alkaline protease was produced from this ochre mutant derivative of apr[BamP] only when the host strain was Su+.
References
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Journal ArticleDOI
Determination of serum proteins by means of the biuret reaction.
TL;DR: An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.
Journal ArticleDOI
The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin.
TL;DR: It is considered simpler and more useful to describe completely the procedures as they are now used in this laboratory, and to avoid confusion about results already published no radical changes have been made.
Journal ArticleDOI
The gel-filtration behaviour of proteins related to their molecular weights over a wide range
TL;DR: Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5.
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The spectrophotometric determination of tyrosine and tryptophan in proteins.
TL;DR: (All right. re8erved)
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Comparison of the specificities of various neutral proteinases from microorganisms
TL;DR: The results led to the consideration that neutral proteinases from microorganisms exhibit remarkably similar although not identical specificities irrespective of their origins.