Protein-Sol: a web tool for predicting protein solubility from sequence.

TLDR: The model returns a predicted solubility and an indication of the features which deviate most from average values, and the utility of these additional features is demonstrated with the example of thioredoxin.

Abstract: Motivation Protein solubility is an important property in industrial and therapeutic applications Prediction is a challenge, despite a growing understanding of the relevant physicochemical properties Results Protein-Sol is a web server for predicting protein solubility Using available data for Escherichia coli protein solubility in a cell-free expression system, 35 sequence-based properties are calculated Feature weights are determined from separation of low and high solubility subsets The model returns a predicted solubility and an indication of the features which deviate most from average values Two other properties are profiled in windowed calculation along the sequence: fold propensity, and net segment charge The utility of these additional features is demonstrated with the example of thioredoxin Availability and implementation The Protein-Sol webserver is available at http://protein-solmanchesteracuk Contact jimwarwicker@manchesteracuk