Proteomic analysis of the inhibitory effect of epigallocatechin gallate on lipid accumulation in human HepG2 cells
Zhonghua Liu,Zhonghua Liu,Qin Li,Jianan Huang,Qionglin Liang,Yujun Yan,Haiyan Lin,Wenjun Xiao,Yong Lin,Sheng Zhang,Bin Tan,Guoan Luo +11 more
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TLDR
The proteomic analysis hypothesized that EGCG reduced cellular lipid accumulation in FFA-induced HepG2 cells through the activation of AMP-activated protein kinase (AMPK) resulting from the generation of reactive oxygen species (ROS).Abstract:
Background
(−)-Epigallocatechin-3-gallate (EGCG), the most abundant catechin found in green tea, effectively reduces body weight and tissue and blood lipid accumulation. To explore the mechanism by which EGCG inhibits cellular lipid accumulation in free fatty acid (FFA) induced HepG2 cell culture, we investigated the proteome change of FFA-induced HepG2 cells exposed to EGCG using two-dimensional gel electrophoresis and mass spectrometry.read more
Citations
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Potential Biological Effects of (-)-Epigallocatechin-3-gallate on the Treatment of Nonalcoholic Fatty Liver Disease
TL;DR: The objectives of this paper are to review and discuss the currently known targets, signaling pathways and roles of EGCG that interfere with NAFLD pathogenesis, then providing additional experimental evidence and the foundation for the further studies and clinical applications of E GCG in the prevention and treatment ofNAFLD.
Journal ArticleDOI
Natural alkaloid and polyphenol compounds targeting lipid metabolism: Treatment implications in metabolic diseases.
Can Cheng,Zhenzhen Li,Xu Zhao,Chaoliang Liao,Jing Quan,Ann M. Bode,Ya Cao,Xiangjian Luo,Xiangjian Luo +8 more
TL;DR: Alkaloid and polyphenol are promising candidates for metabolic diseases to ameliorate lipid metabolism abnormalities to improve or even curing lipid metabolism-related diseases.
Journal ArticleDOI
Chardonnay Grape Seed Flour Ameliorates Hepatic Steatosis and Insulin Resistance via Altered Hepatic Gene Expression for Oxidative Stress, Inflammation, and Lipid and Ceramide Synthesis in Diet-Induced Obese Mice
Kun-Ho Seo,Glenn E. Bartley,Christina Tam,Hong-Seok Kim,Dong-Hyeon Kim,Jung-Whan Chon,Hyunsook Kim,Wallace Yokoyama +7 more
TL;DR: The effects of ChrSd supplementation in a HF diet on weight gain, insulin resistance, and progression of hepatic steatosis in DIO mice were associated with modulation of hepatics genes related to oxidative stress, inflammation, ceramide synthesis, and lipid and cholesterol metabolism.
Journal ArticleDOI
Salacia reticulata has therapeutic effects on obesity
Tsutomu Shimada,Tsutomu Shimada,Yuichiro Nakayama,Yuichiro Nakayama,Yukiko Harasawa,Yukiko Harasawa,Hirofumi Matsui,Hiroko Kobayashi,Yoshimichi Sai,Ken-ichi Miyamoto,Shunji Tomatsu,Masaki Aburada +11 more
TL;DR: It is demonstrated that the extract of S. reticulata has therapeutic effects on obesity and metabolic disorders by enhancing lipogenesis genes and suppressing lipolysis genes through the activation of AMPKα in adipocytes.
Journal ArticleDOI
Potency of turmeric (Curcuma longa L.) extract and curcumin as anti-obesity by inhibiting the cholesterol and triglycerides synthesis in HepG2 cells
TL;DR: The C. longa extract is suggested to have the anti-adipogenesis potential on inhibiting the synthesis of triglycerides and cholesterol and lipid droplet formation in HepG2 cell as anti-obesity parameters better than curcumin.
References
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TL;DR: Green tea, green tea catechins, and epigallocatechin gallate (EGCG) have been demonstrated in cell culture and animal models of obesity to reduce adipocyte differentiation and proliferation, lipogenesis, fat mass, body weight, fat absorption, plasma levels of triglycerides, free fatty acids, cholesterol, glucose, insulin and leptin, as well as to increase beta-oxidation and thermogenesis.