Protoporphyrin IX-induced structural and functional changes in human red blood cells, haemoglobin and myoglobin.
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Cites background from "Protoporphyrin IX-induced structura..."
...On the organism level the PS transport to tumors is done by blood carrier proteins such as serum albumin (SA) [5, 22], low and high density lipoproteins [14], and hemoglobin [23]....
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"Protoporphyrin IX-induced structura..." refers methods in this paper
...The αhelical contents of the protein in the presence and absence of the porphyrin were estimated from the spectra according to the relation (Chen et al 1972): Fraction of α-helix = ([θ ]222 + 2340)/– 30300, where [θ ]222 is the ellipticity at 222 nm. 2.9 Assay of peroxidase activities of Hb and…...
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"Protoporphyrin IX-induced structura..." refers background in this paper
...Superoxide radicals are generated in HRP/H2O2/NAD(P)H system (Takayama and Nakano 1977) and these radicals may inhibit the peroxidase activity of HRP (Fridovich 1986)....
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...…radicals are generated in HRP/H2O2/NAD(P)H system (Takayama and Nakano 1977) and these radicals may inhibit the peroxidase activity of HRP (Fridovich 1986). van Steveninck et al (1987, 1988) have postulated that superoxide radical generation is prevented by the porphyrin hematoporphyrin…...
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...Interaction of PP with HRP as demonstrated from this laboratory (Sil and Chakraborti 1997) may be involved in this process, because potentiation of HRP activity has a positive correlation with the extent of binding of the protein with the porphyrin....
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...Drug-protein binding; haemoglobin; myoglobin; protoporphyrin IX; red blood cells ________________ Abbreviations used: cd, Circular dichroism; EPP, erythropoietic protoporphyria; Hb, haemoglobin; HRP, horseradish peroxidase; Mb, oxymyoglobin; PDT, photodynamic therapy; PP, protoporphyrin; RBC, red blood cells; ZPP, zinc protoporphyrin....
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...It is not yet known how porphyrins remove or inhibit generation of superoxide radicals in HRP system....
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