Journal ArticleDOI
Purification and characterization of a novel anti-proliferative lectin from Morus alba L. leaves.
Mundekkad Deepa,Sulochana Priya +1 more
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TLDR
The purified mulberry leaf lectin showed an anti-proliferative activity towards human breast cancer cells and colon cancer cells with a higher potency towards MCF-7 cells.Abstract:
A novel anti-proliferative lectin was purified from Morus alba L. (Mulberry) leaves by a two step chromatographic procedure namely, immobilized metal ion affinity chromatography (IMAC) and convective interaction media (CIM) based anion exchange chromatography. The purified mulberry leaf lectin (MLL) was specific to galactose, galactosamine and N-acetyl galactosamine (GalNAc). MLL was homogenous with a molecular weight of ~56kDa in silver stained SDS-PAGE. The lectin showed RBC agglutination activity up to 40°C and was independent of pH above pH 6. Haemagglutination activity of purified MLL was not dependent on any metal ions. However, with high concentration of trivalent metal ions, Fe3+ and Al3+ and the divalent metal ion Fe2+, a three fold increase in agglutination activity was observed. The purified MLL showed an anti-proliferative activity towards human breast cancer cells (MCF-7) and colon cancer cells (HCT-15) with a higher potency towards MCF-7 cells. This is the first report on the anti-proliferative activity of a GalNAc specific lectin from M. alba.read more
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Immobilized metal ion affinity chromatography: a review on its applications
TL;DR: This review addresses the developments, limitations, and considerations of IMAC in the biopharmaceutical industry.
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Anticancer Plants: A Review of the Active Phytochemicals, Applications in Animal Models, and Regulatory Aspects
Tariq Khan,Muhammad Ali,Ajmal Khan,Parveen Nisar,Sohail Ahmad Jan,Shakeeb Afridi,Zabta Khan Shinwari +6 more
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Journal ArticleDOI
Purified mulberry leaf lectin (MLL) induces apoptosis and cell cycle arrest in human breast cancer and colon cancer cells.
TL;DR: Investigation of the mechanism of cell death induction by MLL on human breast cancer and colon cancer cells found that MLL induced apoptosis in MCF-7 and HCT-15 cells in a caspase dependent manner.
Journal ArticleDOI
Microencapsulation of lectin anti-cancer agent and controlled release by alginate beads, biosafety approach.
TL;DR: The extracted lectins showed anti-proliferative effect against HCC with a percentage of 60.76% by using its nontoxic dose with an up-regulation of P53 gene expression and this solid dosage form could be suitable for oral administration complemented with/or without the standard HCC drugs.
Journal ArticleDOI
Mori folium inhibits interleukin-1β-induced expression of matrix metalloproteinases and inflammatory mediators by suppressing the activation of NF-κB and p38 MAPK in SW1353 human chondrocytes.
Jin-Woo Jeong,Hye Hyeon Lee,Ki Won Lee,Ki-Young Kim,Sung Goo Kim,Su Hyun Hong,Gi-Young Kim,Cheol Park,Ho Kyoung Kim,Young-Whan Choi,Yung Hyun Choi +10 more
TL;DR: The novel findings of the present study suggest that Mori folium water extract is a potential therapeutic choice for chondroprotection against the collagen matrix breakdown in the cartilage of diseased tissues, such as those found in patients with arthritic disorders.