Journal ArticleDOI
Purification and characterization of two forms of extracellular β-glucosidase from jute pathogenic fungus Macrophomina phaseolina
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TLDR
Two forms of β-glucosidase are separated and partially purified by (NH4)2SO4 precipitation, ion-exchange chromatography (DE-52) and gel filtration and may be differentiated by inhibition experiments using inhibitors like glucono-δ-lactone and nojirimycin.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1981-11-13. It has received 8 citations till now. The article focuses on the topics: Nojirimycin & Macrophomina phaseolina.read more
Citations
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Journal ArticleDOI
The Properties of Fungal and Bacterial Cellulases with Comment on their Production and Application
TL;DR: Structure de la cellulose, sources et production d'enzymes cellulolytiques: regulation, secretion, mutation, mutation et clonage, multiplicite des enzymes.
Journal ArticleDOI
Properties of β-glucosidase purified from Aspergillus niger mutants USDB 0827 and USDB 0828
TL;DR: Two extracellular β-glucosidases were isolated from Aspergillus nigerUSDB 0827 and A. niger USDB 0828 and were very similar in terms of molecular size, pH optimum, temperature optimum, stability at high temperatures and substrate preferences, but differed in inhibition by glucono-1,5-lactone and affinity for cellobiose.
Journal ArticleDOI
Extracellular cellulolytic enzyme system of Aspergillus japonicus: 2. Purification and characterization of an inducible extracellular β-glucosidase
TL;DR: A high molecular weight β-glucosidase was isolated from the culture filtrate of Aspergillus japonicus and was finally purified to 86-fold by alcohol precipitation, gel filtration and ion-exchange chromatography on Whatman DE-52.
Journal ArticleDOI
Effect of glucose and other sugars on the β-1,4-glucosidase activity of Thermomonospora fusca
John D. Ferchak,E. Kendall Pye +1 more
TL;DR: The cell‐associated β‐glucosidase activity of Thermomonospora fusca, strain YX, showed both PNPGase and cellobiase activities, and growth experiments gave further evidence for the probability of separate enzymes for the PN PGase andcellobiases activities.
Journal ArticleDOI
A novel Trichoderma harzianum strain from the Amazon Forest with high cellulolytic capacity
TL;DR: The association of a high cellulolytic activity and a high β-glucosidase activity in the same microorganism indicates the industrial potential of this new wild type T. harzianum strain.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Book
Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
Structure and synthesis of nojirimycin.
TL;DR: Nojirimycin, an antibiotic produced by several Streptomyces has been shown to be d -gluco-piperidinose (5-amino-5-deoxy- d -glucopyranose) on the basis of chemical and spectroscopic evidence and synthesis.
Journal ArticleDOI
Macroconidium Formation in Submerged Cultures by a Nonsporulating Strain of Gibberella Zeae
R. A. Cappellini,J. L. Peterson +1 more
TL;DR: The typical New Jersey corn stalk-rotting strain of G. zeae does not sporulate in laboratory surface culture, and produces abundant, typical macrospores only in shake-based culture.