Journal ArticleDOI
Purification of oligouronides by immobilized L-histidine pseudoaffinity chromatography.
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TLDR
This new monolithic CIM-disk system with L-histidine immobilized: immobilized histidine affinity chromatography (IHAC) constitutes a good tool allowing the fast and selective purification of bioactive oligouronides.About:
This article is published in Journal of Chromatography B.The article was published on 2008-01-15. It has received 14 citations till now. The article focuses on the topics: Elution & Sepharose.read more
Citations
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Performance of a non-grafted monolithic support for purification of supercoiled plasmid DNA.
TL;DR: The binding capacity has been found to be significantly higher for sc plasmid, probably because of its compact structure, being also improved when using feedstock with increased plasmids concentrations and decreased linear velocity.
Journal ArticleDOI
Macroporous monolithic supports for affinity chromatography
TL;DR: This mini-review reports the contributions of several groups to the development of macroporous monoliths and their modification by immobilization of specific ligands on the products for their application in affinity chromatography.
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Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention.
TL;DR: One of the most striking results shows that histidine interacts preferentially with guanine, and the presence of secondary structures on polyA and polyG oligonucleotides has a significant influence on retention.
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Screening nucleotide binding to amino acid-coated supports by surface plasmon resonance and nuclear magnetic resonance
TL;DR: A rapid and efficient screening method using surface plasmon resonance (SPR) and saturation transfer difference–nuclear magnetic resonance (STD-NMR) spectroscopy to yield information regarding the residues involved in nucleotide binding to amino acid-coated supports is described.
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Analysis of hSCOMT adsorption in bioaffinity chromatography with immobilized amino acids: The influence of pH and ionic strength
TL;DR: The results suggest that the AAILs-hSComT interaction is strongly affected by the intrinsic hSCOMT hydrophobic domains, and a new approach in biochromatographic COMT retention, while possess a higher specificity than other chromatographic methods reported in literature.
References
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Biological roles of oligosaccharides: all of the theories are correct
TL;DR: The only common features of the varied functions of oligosaccharides are that they either mediate ‘specific recognition’ events or that they provide ‘modulation’ of biological processes.
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Pectins: structure, biosynthesis, and oligogalacturonide-related signaling.
TL;DR: The view of critical questions regarding pectin structure, biosynthesis, and function that need to be addressed in the coming decade are presented and new methods that may be useful to study localized pectins in the plant cell wall are described.
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Sulfated fucans, fresh perspectives : structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide
Olivier Berteau,Barbara Mulloy +1 more
TL;DR: The availability of purified fucans and fucan fractions with simple, but varied structures, in conjunction with the development of new enzymatic tools, demonstrate that the biological properties of sulfation are not only a simple function of their charge density but also are determined by detailed structural features.
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ALGINATE LYASE: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications.
TL;DR: Characterization of alginate lyases will enhance and expand the use of these enzymes to engineer novelAlginate polymers for applications in various industrial, agricultural, and medical fields.
Journal ArticleDOI
Linear β-1,3 Glucans Are Elicitors of Defense Responses in Tobacco
Olivier Klarzynski,Bertrand Plesse,Jean-Marie Joubert,Jean-Claude Yvin,Marguerite Kopp,Bernard Kloareg,Bernard Fritig +6 more
TL;DR: Structural activity studies showed that the elicitor effects observed in tobacco with β-glucans are specific to linear β-1,3 linkages, with laminaripentaose being the smallest elicitor-active structure.