Purification of RuBisCO from the thermophilic cyanobacterium Synechococcus sp. strain a-1
TL;DR: Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), the key enzyme of the Calvin Benson cycle, has been purified from a thermophilic cyanobacterium, Synechococcus sp.
About: This article is published in Journal of Fermentation and Bioengineering.The article was published on 1992-01-01. It has received 19 citations till now. The article focuses on the topics: RuBisCO & Light-independent reactions.
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TL;DR: Two putative ATP-binding proteins encoded in the gene cluster for the Calvin cycle of Pseudomonas hydrogenothermophila (cbbQ) and for the denitrification of PseUDomonas aeruginosa (nirQ) have been found to be similar.
28Â citations
Cites methods from "Purification of RuBisCO from the th..."
...The enzyme assay for RubisCO was performed by using NaH(14)CO3 at 503C as described previously [17]....
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TL;DR: Investigation of three sets of genes for ribulose 1,5-bisphosphate carboxylase/oxygenase in Hydrogenovibrio marinus strain MH-110 revealed that the specific activity of CbbM was very low and CBBM was inactivated easily during the process of purification, and the antibody to neither form of RubisCO demonstrated cross-reactivity for the other form.
25Â citations
TL;DR: The N-terminal amino acid sequence determination of the purified enzyme showed high homology with those of the L2-form RubisCO of Rhodospirillum rubrum and the Lx-formRubisCO from Rhodobacter sphaeroides.
Abstract: Ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) was purified from an obligately autotrophic hydrogen-oxidizing bacterium, Hydrogenovibrio marinus MH-110. The protein has a Mr value of approximately 110 000, and is composed of two identical subunits of 55 000. To our knowledge, the existence of L2-form RubisCO in a chemolithoautotrophic bacterium is first reported in this paper. The N-terminal amino acid sequence determination of the purified enzyme showed high homology with those of the L2-form RubisCO of Rhodospirillum rubrum and the Lx-form RubisCO from Rhodobacter sphaeroides.
20Â citations
TL;DR: An ammonia-oxidizing bacterium was isolated from the rhizoplane of the reed used in an aquaponics plant which is a wastewater treatment plant and had a higher activity than that of Nitrosomonas europaea ATCC25978T.
20Â citations
TL;DR: Two cyanobacterial strains obtained from hot springs of Northern Thailand belong to genus Chroococcidiopsis, and properties indicate that the strain can be used for the conversion to organic matter of carbon dioxide produced by combustion.
18Â citations
References
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TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Abstract: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.
232,912Â citations
Journal Article•
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Abstract: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.
203,017Â citations
TL;DR: A cloned fragment of spinach chloroplast DNA carrying the gene for the large subunit of ribulose bisphosphate (RuBP) carboxylase has been analysed by electron microscopy of R-loops, by hybridization to Northern blots of chloropleft RNA, by S1 nuclease mapping and by DNA sequencing.
Abstract: A cloned fragment of spinach chloroplast DNA carrying the gene for the large subunit of ribulose bisphosphate (RuBP) carboxylase has been analysed by electron microscopy of R-loops, by hybridization to Northern blots of chloroplast RNA, by S1 nuclease mapping and by DNA sequencing. The transcribed region of the gene is 1690 +/- 3 nucleotides long and co-linear with its mRNA. It comprises a 178-179 bp 5' untranslated sequence, a 1425 bp coding region and an 85-88 bp 3' untranslated region. The deduced sequence of the 475 amino acids of the spinach large subunit protein shows 10% divergence from that of the maize large subunit protein (1). The nucleotide sequence divergence between spinach and maize over the same coding region is 16% but in the transcribed flanking regions it is 35%. Features of the spinach chloroplast gene which resemble those of bacterial genes include a 5-base Shine-Dalgarno sequence complementary to a sequence near the 3' end of chloroplast and bacterial 16S rRNA, a promoter region partially homologous to a consensus sequence of bacterial promoters, and a transcription termination region capable of forming a typical stem and loop structure.
376Â citations
TL;DR: This chapter focuses on the culturing methods for cyanobacteria, and identifies three major systems of classification: Geitler, Drouet, and Stanier systems.
Abstract: Publisher Summary This chapter focuses on the culturing methods for cyanobacteria. For the establishment of new cultures, as much ecological information as possible about the field populations should be gathered and recorded. With this, it eventually becomes possible to relate diverse information obtained from culture strains to the survival strategies of the organism in nature, ultimately the object of all biological research unless it is directed solely to the benefit of humans. Cyanobacteria, unlike most other prokaryotes (eubacteria or archaebacteria) are usually seen and often recognizable (even at the species level) before collection; they are present in many cases as plankton blooms or as dense tufts or mats which are composed of few other species. Only a miniscule percentage of cyanobacterial species have been brought into culture. This, in part, reflects the relatively few and mainly recent attempts to isolate and grow diverse cyanobacteria. Cyanobacteria from certain habitats and those of certain taxonomic groups seem to be extremely recalcitrant with regard to being cultured with present methods. Identification of cyanobacteria, whether of natural populations or of cultured material, is not a simple task, because at present, there are three major systems of classification: Geitler, Drouet, and Stanier systems.
360Â citations
TL;DR: Molecular Approaches to the Function of Form I and Form II RuBPC/O, PRK, and FBP in Rhodobacter sphaeroides and Regulatory Determinants are studied.
256Â citations