Pushing the Limits of MALDI-TOF Mass Spectrometry: Beyond Fungal Species Identification
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TLDR
In this article, a review of the use of MALDI-TOF in the clinical mycology laboratory is presented, focusing on present and future applications of this versatile analytical tool in the field of bioinformatics.Abstract:
Matrix assisted laser desorption ionization time of flight (MALDI-TOF) is a powerful analytical tool that has revolutionized microbial identification. Routinely used for bacterial identification, MALDI-TOF has recently been applied to both yeast and filamentous fungi, confirming its pivotal role in the rapid and reliable diagnosis of infections. Subspecies-level identification holds an important role in epidemiological investigations aimed at tracing virulent or drug resistant clones. This review focuses on present and future applications of this versatile tool in the clinical mycology laboratory.read more
Citations
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Journal ArticleDOI
Molecular diagnostics in medical mycology
TL;DR: Current and future molecular technologies used for fungal identification, and some of the problems associated with development and implementation of these technologies in today’s clinical microbiology laboratories are discussed.
Journal ArticleDOI
Online Databases for Taxonomy and Identification of Pathogenic Fungi and Proposal for a Cloud-Based Dynamic Data Network Platform.
Peralam Yegneswaran Prakash,Peralam Yegneswaran Prakash,Peralam Yegneswaran Prakash,Laszlo Irinyi,Catriona Halliday,Sharon C.-A. Chen,Sharon C.-A. Chen,Vincent Robert,Wieland Meyer +8 more
TL;DR: This minireview aims to provide an overview of currently available online databases for the taxonomy and identification of human and animal-pathogenic fungi and calls for the establishment of a cloud-based dynamic data network platform.
Journal ArticleDOI
New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples.
TL;DR: New molecular-based approaches for detecting triazole resistance to Aspergillus, real-time polymerase chain reaction (PCR) to detect mutations to the Cyp51A protein, have been developed which are able to detect mostTriazole-resistant A. fumigatus strains in patients with invasive aspergillosis.
Journal ArticleDOI
New method for the identification of arbuscular mycorrhizal fungi by proteomic-based biotyping of spores using MALDI-TOF-MS
Thomas Crossay,Cyril Antheaume,Dirk Redecker,Lucie Bon,Nicolas Chedri,Clément Richert,Linda Guentas,Yvon Cavaloc,Hamid Amir +8 more
TL;DR: Fast, accurate and inexpensive molecular mass determination and the possibility of automation make MALDI-TOF-MS a real alternative to conventional morphological and molecular methods for AMF identification.
Journal ArticleDOI
MALDI-TOF mass spectroscopy of yeasts and filamentous fungi for research and diagnostics in the agricultural value chain
TL;DR: The role of MALDI-TOF MS as a tool for species identification; in particular with respect to DNA-based identification methods is discussed, and the value of custom-made reference spectra for MalDI biotyping is highlighted.
References
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MALDI-TOF typing highlights geographical and fluconazole resistance clusters in Candida glabrata
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