Pushing the Limits of MALDI-TOF Mass Spectrometry: Beyond Fungal Species Identification
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TLDR
In this article, a review of the use of MALDI-TOF in the clinical mycology laboratory is presented, focusing on present and future applications of this versatile analytical tool in the field of bioinformatics.Abstract:
Matrix assisted laser desorption ionization time of flight (MALDI-TOF) is a powerful analytical tool that has revolutionized microbial identification. Routinely used for bacterial identification, MALDI-TOF has recently been applied to both yeast and filamentous fungi, confirming its pivotal role in the rapid and reliable diagnosis of infections. Subspecies-level identification holds an important role in epidemiological investigations aimed at tracing virulent or drug resistant clones. This review focuses on present and future applications of this versatile tool in the clinical mycology laboratory.read more
Citations
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Journal ArticleDOI
Molecular diagnostics in medical mycology
TL;DR: Current and future molecular technologies used for fungal identification, and some of the problems associated with development and implementation of these technologies in today’s clinical microbiology laboratories are discussed.
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Online Databases for Taxonomy and Identification of Pathogenic Fungi and Proposal for a Cloud-Based Dynamic Data Network Platform.
Peralam Yegneswaran Prakash,Peralam Yegneswaran Prakash,Peralam Yegneswaran Prakash,Laszlo Irinyi,Catriona Halliday,Sharon C.-A. Chen,Sharon C.-A. Chen,Vincent Robert,Wieland Meyer +8 more
TL;DR: This minireview aims to provide an overview of currently available online databases for the taxonomy and identification of human and animal-pathogenic fungi and calls for the establishment of a cloud-based dynamic data network platform.
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New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples.
TL;DR: New molecular-based approaches for detecting triazole resistance to Aspergillus, real-time polymerase chain reaction (PCR) to detect mutations to the Cyp51A protein, have been developed which are able to detect mostTriazole-resistant A. fumigatus strains in patients with invasive aspergillosis.
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New method for the identification of arbuscular mycorrhizal fungi by proteomic-based biotyping of spores using MALDI-TOF-MS
Thomas Crossay,Cyril Antheaume,Dirk Redecker,Lucie Bon,Nicolas Chedri,Clément Richert,Linda Guentas,Yvon Cavaloc,Hamid Amir +8 more
TL;DR: Fast, accurate and inexpensive molecular mass determination and the possibility of automation make MALDI-TOF-MS a real alternative to conventional morphological and molecular methods for AMF identification.
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MALDI-TOF mass spectroscopy of yeasts and filamentous fungi for research and diagnostics in the agricultural value chain
TL;DR: The role of MALDI-TOF MS as a tool for species identification; in particular with respect to DNA-based identification methods is discussed, and the value of custom-made reference spectra for MalDI biotyping is highlighted.
References
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Journal ArticleDOI
MALDI-TOF mass signatures for differentiation of yeast species, strain grouping and monitoring of morphogenesis markers
TL;DR: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is demonstrated to be a potentially useful tool for the rapid identification of yeasts, the grouping of Candida albicans strains, and the monitoring of germ tube-specific markers.
Journal ArticleDOI
Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Caspofungin Susceptibility Testing of Candida and Aspergillus Species
Elena De Carolis,Antonietta Vella,Ada Rita Florio,Patrizia Posteraro,David S. Perlin,Maurizio Sanguinetti,Brunella Posteraro +6 more
TL;DR: In this paper, a matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin of wild-type and fks mutant isolates of Candida and Aspergillus.
Journal ArticleDOI
Identification of filamentous fungi isolates by MALDI-TOF mass spectrometry: clinical evaluation of an extended reference spectra library.
Pierre Becker,Annelies De Bel,Delphine Martiny,Stéphane Ranque,Renaud Piarroux,Carole Cassagne,Monique Detandt,Marijke Hendrickx +7 more
TL;DR: A large in-house library containing 760 strains and representing 472 species was built and evaluated by comparing MALDI-TOF MS with the classical identification method based on morphological observations, appearing superior to morphology in order to obtain a robust and accurate identification of filamentous fungi.
Journal ArticleDOI
Improved Identification of Yeast Species Directly from Positive Blood Culture Media by Combining Sepsityper Specimen Processing and Microflex Analysis with the Matrix-Assisted Laser Desorption Ionization Biotyper System
Yingjun Yan,Ying He,Thomas Maier,Criziel D. Quinn,Gongyi Shi,Haijing Li,Charles W. Stratton,Markus Kostrzewa,Yi-Wei Tang +8 more
TL;DR: The data indicated that the Sepsityper specimen processing and Microflex analysis by the MALDI Biotyper system provide a rapid and reliable tool for yeast species identification directly from positive blood culture media.
Journal ArticleDOI
Universal In Vitro Antifungal Resistance of Genetic Clades of the Fusarium solani Species Complex
TL;DR: Eleven antifungal drugs were tested against representative isolates of the four phylogenetic clades of the Fusarium solani species complex obtained in a multilocus sequence analysis, and all showed very poor activity.
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