Quality evaluation of traditional Chinese drug toad venom from different origins through a simultaneous determination of bufogenins and indole alkaloids by HPLC.
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TLDR
A simple and efficient HPLC method has been developed to evaluate the quality of traditional Chinese medicine toad venoms, and Toad venom samples from B. bufo gargarizans from median China were considered to be of the highest quality.Abstract:
A simple and efficient HPLC method has been developed to evaluate the quality of traditional Chinese medicine toad venoms. The major bioactive ingredients, including 10 bufogenins and 4 indole alkaloids in the drug, were separated and quantified on a phenyl-hexyl column with a UV detector. A total of 27 toad venom samples from two species, Bufo bufo gargarizans CANTOR and Bufo melanostictus SCHNEIDER, from the different drug production regions of China, were analyzed. The chromatograms showed significant differences with respect to the samples from different origins. These toad venom samples can be distinctly classified into 4 groups by cluster analysis using the contents of the 14 main constituents, including toad venom samples from B. bufo gargarizans from north China, median China and south China, and samples from B. melanostictus from south China. Toad venom samples from B. bufo gargarizans from median China were considered to be of the highest quality.read more
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References
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Journal ArticleDOI
Bufadienolides from animal and plant sources.
Liselotte Krenn,Brigitte Kopp +1 more
TL;DR: Naturally occurring bufadienolides, which were isolated from both animal and plant sources and structurally elucidated in the period from 1967-1995, are reviewed and compiled.
Journal ArticleDOI
Toxicity of Panamanian poison frogs (Dendrobates): some biological and chemical aspects
John W. Daly,Charles W. Myers +1 more
TL;DR: A small Neotropical frog, Dendrobates pumilio, undergoes interpopulational variation in color, degree of toxicity, size, and habits, which may result from isolation and chance restriction of original heterozygosity, with subsequent selection acting on different and greatly limited mixtures of alleles.
Journal ArticleDOI
QSAR evaluation of the Ch'an Su and related bufadienolides against the colchicine-resistant primary liver carcinoma cell line PLC/PRF/5(1).
Yoshiaki Kamano,Ayano Yamashita,Toshihiko Nogawa,Hiroshi Morita,Koichi Takeya,Hideji Itokawa,Toshiaki Segawa,Ayako Yukita,Kyoko Saito,Mariko Katsuyama,George R. Pettit +10 more
TL;DR: QSAR analysis has been used to identify the essential structural requirements for increasing the inhibitory activities of selected bufadienolides from the Chinese drug Ch'an Su against the primary liver carcinoma cell line PLC/PRF/5 (PLC) and the derived colchicine-resistant line (COL).
Journal ArticleDOI
Characterisation and determination of indole alkaloids in frog-skin secretions by electrospray ionisation ion trap mass spectrometry.
TL;DR: The quantitative determination of certain indole alkaloids in the skin secretions of the Australian Golden Bell frog, Litoria aurea, by LC/ESI-ion trap MS is presented.
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Determination of potentially hallucinogenic N-dimethylated indoleamines in human urine by HPLC/ESI-MS-MS
TL;DR: The bufotenine excretion of psychiatric patients was found to be higher than that of the somatic patients, and the dimethylated amines were found in significant amounts.