Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells.
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...Such strains provide a direct readout of protein abundance and have led to large-scale surveys of protein expression within single cell...
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...Besides the above methods, the large-scale protein turnover in mammalian cells has been measured using, e.g., fluorescently tagged proteins combined by fluorescence microscopy (Yen et al., 2008; Taniguchi et al., 2010; Eden et al., 2011)....
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...…on Cell Populations versus Single Cells of One Cell Type Despite the rapid progress for measuring mRNA and protein levels in single cells, it remains exceedingly difficult to measure both types of molecules in the same cells at high throughput (Darmanis et al., 2016; Taniguchi et al., 2010)....
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References
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