Quantitative distribution and localization of calcitonin gene-related peptide-like cells in the stomach of two kidney, one clip rats.

Home
/
Papers
/
Quantitative distribution and localization of calcitonin gene-related peptide-like cells in the stomach of two kidney, one clip rats.

Journal Article•

Quantitative distribution and localization of calcitonin gene-related peptide-like cells in the stomach of two kidney, one clip rats.

Irena Kasacka¹•Institutions (1)

01 Jun 2009-Journal of Physiology and Pharmacology (J Physiol Pharmacol)-Vol. 60, Iss: 2, pp 35-39

TL;DR: The aim of the present study was to examine the number and distribution of CGRP-containing cells in the pylorus of "two kidney, one clip" (2K1C) renovascular hypertension model in rats and the changes induced by hypertension in the neuroendocrine cells containing C GRP of the rats.

read less

Abstract: The majority of research for the calcitonin gene-related peptide (CGRP) in the stomach in the hypertension has been devoted to the submucosal blood flow, and no attention has been paid to its quantitative distribution in the gastric neuroendocrine cells. The aim of the present study was to examine the number and distribution of CGRP-containing cells in the pylorus of "two kidney, one clip" (2K1C) renovascular hypertension model in rats. The studies were carried out on the stomach of rats. After 6 week period of the renal artery clipping procedure, eight 2K1C rats developed stable hypertension. The hypertension significantly increased the number of endocrine cells pylorus immunoreactive to calcitonin gene-related peptide (CGRP) antisera. The differences between the hypertensive rats and the control group concerned not only the number of endocrine cells but also their distribution. CGRP participates in the regulation of cardiovascular functions both in normal state and in the pathophysiology of hypertension through interactions with the prohypertensive systems. The changes induced by hypertension in the neuroendocrine cells containing CGRP of the rats are discussed.

...read moreread less

Content maybe subject to copyright Report

Citations

PDF

Open Access

More filters

Journal Article•DOI•

Changes in VIP-, SP- and CGRP- like immunoreactivity in intramural neurons within the pig stomach following supplementation with low and high doses of acrylamide.

[...]

Katarzyna Palus¹, Michał Bulc¹, Jarosław Całka¹•Institutions (1)

University of Warmia and Mazury in Olsztyn¹

01 Dec 2018-Neurotoxicology

TL;DR: It has been shown that acrylamide induces a significant response of ENS neurons even in TDI dose, which suggests that it is not neutral to the body.

...read moreread less

Abstract: Acrylamide is one of the food toxins to which the human body is exposed. Although researchers’ interest in acrylamide has been growing in recent years, the knowledge of its effect on the gastrointestinal tract, especially on intramural neurons which form the enteric nervous system is scarce. The aim of this experiment was to determine the influence of acrylamide, administered at doses equivalent to the human tolerable daily intake (TDI, 0.5 μg/kg b.w./day) and ten times higher than the TDI (5 μg/kg b.w./day), on the distribution of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene related peptide (CGRP) in intramural neurons of the domestic pig stomach. Using double immunofluorescent labelling we revealed that the ENS neurons underwent adaptive changes in response to the supplementation of acrylamide, which manifested themselves as increased expression of VIP, SP and CGRP, both in intramural neurons and by an increase in the nerve density in submucous and muscular layers in the porcine stomach. These substances take part in defensive reactions of neurons and transmission of sensory reactions may play an important role in protecting the stomach against the harmful effect of acrylamide. Moreover, it has been shown that acrylamide induces a significant response of ENS neurons even in TDI dose, which suggests that it is not neutral to the body. These findings may be the basis for further toxicological studies addressing the question if currently permitted minimal content of acrylamide in the food does jeopardize the health of human consumers?

...read moreread less

20 citations

Cites background or result from "Quantitative distribution and local..."

...In addition, calcitonin gene related peptide (CGRP) was originally identified as a product of alternative processing of RNA of the calcitonin gene in the rat brain and is common in neurons and nerves in the gastrointestinal tract as well as in the central and peripheral nervous system (Hayakawa et al., 2009; Kasacka, 2009; Lee and Nam, 2006; Timmermans et al., 1992; Toole et al., 1998)....
[...]
...…of alternative processing of RNA of the calcitonin gene in the rat brain and is common in neurons and nerves in the gastrointestinal tract as well as in the central and peripheral nervous system (Hayakawa et al., 2009; Kasacka, 2009; Lee and Nam, 2006; Timmermans et al., 1992; Toole et al., 1998)....
[...]
...These findings are similar to those of earlier studies on rodents, mammals and humans (Kaleczyc et al., 2007; Kasacka, 2009; Pimont et al., 2003; Schemann et al., 2001)....
[...]

Journal Article•DOI•

Evaluation of density and distribution of CART-immunoreactive structures in gastrointestinal tract of hypertensive rats.

[...]

Irena Kasacka¹, Żaneta Piotrowska¹•Institutions (1)

Medical University of Białystok¹

01 Nov 2012-Biofactors

TL;DR: This study aims to identify, localize, and assess changes in CART-immunopositive structures of the gastrointestinal tract (GI tract) of rats with renovascular hypertension, and detects CART in the extensive population of neurons and in neuroendocrine cells.

...read moreread less

Abstract: The prevalence of CART (cocaine- and amphetamine-regulated transcript) throughout the organism, multiplicity of functions fulfilled by that peptide, and the collected evidence confirming CART contribution to blood pressure regulation prompted us to undertake the research aiming to identify, localize, and assess changes in CART-immunopositive structures of the gastrointestinal tract (GI tract) of rats with renovascular hypertension. The two-kidney one-clip model of arterial hypertension was used to evaluate the location and density of CART-containing structures in the stomach (cardia, fundus, and pylorus), duodenum, jejunum, ileum, and colon of hypertensive rats. The study was carried out on the GI tract of 20 rats. Ten rats were subjected to the renal artery clipping procedure and after a 6-week period each of them developed stable hypertension. An immunohistochemical localization of CART was performed on paraffin GI tract sections from all the study animals. CART was detected in the extensive population of neurons, particularly within the myenteric plexuses all along the GI tract, and also in neuroendocrine cells, being especially numerous in the stomach and a few in the small intestine. The hypertension significantly increased the density of CART-positive structures in the rat GI tract. The differences between the hypertensive rats and the control animals concerned not only the density of CART-immunoreactive structures but also the staining intensity. As this study provides novel findings, we are planning further molecular examinations to better understand the impact of hypertension on the functioning and activity of CART in the GI tract. © 2012 International Union of Biochemistry and Molecular Biology, Inc.

...read moreread less

19 citations

Cites background or result from "Quantitative distribution and local..."

...Considering that in previous research we proved likewise greater number of CGRP-IR endocrine cells in the stomach of 2K1C rats when compared with the control group, we might suggest the impaired protective and secretory mechanisms in the hypertensive rats [16]....
[...]
...There is some clinical and experimental evidence for the involvement of neuropeptides and proinflammatory markers in the pathogenesis of arterial hypertension [16,34,36]....
[...]
...The experimental data, and also numerous clinical observations, indicate a significant role of humoral factors and neurotransmitters in the pathogenesis of arterial hypertension [9,16]....
[...]
...As there is evidence [16,17] confirming the influence of hypertension on the activity of CGRP-containing cells, considerable changes may be expected in the content of CART peptide in animals with renovascular hypertension....
[...]

Journal Article•DOI•

Alterations of rat stomach endocrine cells under renovascular hypertension.

[...]

Irena Kasacka¹, Zaneta Piotrowska¹, Alicja Lewandowska¹•Institutions (1)

Medical University of Białystok¹

01 Sep 2014-Advances in Medical Sciences

TL;DR: Investigations demonstrated the impact of renovascular hypertension on the neuroendocrine system in the rat stomach and changes in the total number and ultrastructure of DNES cells proved their undeniable role in the modulation of gastric dysfunction, as a consequence of deregulation of homeostasis-maintaining systems.

...read moreread less

7 citations

Cites background from "Quantitative distribution and local..."

...activity of parietal cells and blood flow through the gastric mucosa [1,2,27]....
[...]
...Many investigations have indicated that in various pathological ates the number and morphology of NE cells in the stomach dergo some changes [1,2,12]....
[...]
...Several experimental studies have demonstrated that hypertension causes disturbances in the secretion of neuropeptides and biogenic amines in the majority of organs, including the digestive system [1,2]....
[...]
...This seems to confirm the volvement of biologically active substances produced by stroendocrine cells in various pathological and adaptive ocesses in the body [1,2,12]....
[...]

Journal Article•DOI•

Dynamics of calcitonin gene-related peptide-like cells changes in the lungs of two-kidney, one-clip rats.

[...]

Irena Kasacka¹, Ewa Arciszewska¹•Institutions (1)

Medical University of Białystok¹

08 Mar 2012-European Journal of Histochemistry

TL;DR: It was revealed that renovascular hypertension caused changes in the neuroendocrine, CGRP-containing cells in the lungs of rats in the two-kidney, one-clip (2K1C) renov vascular hypertension model.

...read moreread less

Abstract: Taking into consideration renal hypertension-induced homeostatic disorders and the key role of calcitonin gene-related peptide (CGRP)Â in many, systemic functions regulating systems, a question arises as to what an extent arterial hypertension affects the morphology and dynamics of pulmonary CGRP-immunopositive cell changes. The aim of the present study was to examine the distribution, morphology and dynamics of changes of CGRP-containing cells in the lungs of rats in the two-kidney, one-clip (2K1C) renovascular hypertension model. The studies were carried out on the lungs of rats after 3, 14, 28, 42, and 91 days long period from the renal artery clipping procedure. In order to identify neuroendocrine cells, immunohistochemical reaction was performed with the use of a specific antibody against CGRP. It was revealed that renovascular hypertension caused changes in the neuroendocrine, CGRP-containing cells in the lungs of rats. The changes, observed in the neuroendocrine cells, depended on time periods from experimentally induced hypertension. The highest intensity of changes in the neuroendocrine cells was observed in the lungs of rats after 14 days from the surgery.

...read moreread less

6 citations

Journal Article•DOI•

Gastrointestinal neuroendocrine cells in various types of hypertension –a review

[...]

M. Niezgoda¹, I. Kasacka¹•Institutions (1)

Medical University of Białystok¹

29 Dec 2017-Progress in Health Sciences

TL;DR: Results obtained in many research studies indicate intense activity of enteroendocrine cells in the gastrointestinal tract in various pathological conditions, including hypertension.

...read moreread less

Abstract: ____________________________________________________________________________________________________ Recent years have witnessed a progressive increase in the number of people suffering from hypertension, which is one of the most serious health problems in the world. Hypertension results in changes leading to function disorders, not only of the organs and tissues, but also changes leading to the activation of many defense mechanisms in the cells in order to prevent damage. One of them is the expression of neuroendocrine (NE) hormones and biologically active substances, which has been the focus of extensive research for a number of years. Active involvement of NE cells and the biological and therapeutic properties of various substances synthesized by them have been confirmed in clinical trials and in various experimental models. Results obtained in many research studies indicate intense activity of enteroendocrine cells in the gastrointestinal tract in various pathological conditions, including hypertension. In the present review, we discuss the morphological and functional changes of gastrointestinal neuroendocrine cells under conditions of different types of hypertension.

...read moreread less

6 citations

Cites background from "Quantitative distribution and local..."

...This illustrates the important role played by these cells in maintaining whole body homeostasis [45,46,47]....
[...]

References

PDF

Open Access

More filters

Journal Article•DOI•

Relaxant effects of calcitonin gene-related peptide on isolated small renal arteries in stroke-prone spontaneously hypertensive rats.

[...]

Yujing Gao¹, Yoshitaka Nishimura¹, Aritomo Suzuki¹, Kazumi Yoshida¹•Institutions (1)

Kindai University¹

01 Feb 1994-Journal of Smooth Muscle Research

TL;DR: It is suggested that C GRP produces endothelium-independent relaxation in the small renal arteries in the rat, and that the increased CGRP-induced relaxant response found in SHRSP may not be associated with the altered vasodilation mediated by cyclic AMP, or with functional changes in ATP-sensitive potassium channels.

...read moreread less

Abstract: The relaxant effects of calcitonin gene-related peptide (CGRP) on the 3rd branches of renal arteries obtained from stroke-prone spontaneously hypertensive rats (SHRSP), and Wistar-Kyoto rats (WKY) were investigated in vitro. CGRP elicited concentration-dependent relaxation, and the relaxant response was not affected by the mechanical removal of endothelium in either SHRSP or WKY. The CGRP-induced relaxant response was markedly greater in SHRSP than in WKY, whereas there was no significant difference in acetylcholine-induced relaxation, which was endothelium-dependent, between the two groups. Additionally, significantly enhanced reactivity to CGRP was also shown in sponta-neously hypertensive rats compared to WKY; however, this reactivity was less than that observed in SHRSP.There were also no significant differences between WKY and SHRSP in the relaxation induced by forskolin, dibutyryl cyclic AMP, and 3-isobutyl-1-methylxanthine (IBMX). CGRP-induced relaxation was significantly potentiated in similar manner by the pretreatment with IBMX in both WKY and SHRSP.Incubation with glibenclamide (10-6 M) had no effect on CGRP-induced relaxation in either group, the WKY or the SHRSP. These results suggest that CGRP produces endothelium-independent relaxation in the small renal arteries in the rat, and that the increased CGRP-induced relaxant response found in SHRSP may not be associated with the altered vasodilation mediated by cyclic AMP, or with functional changes in ATP-sensitive potassium channels.

...read moreread less

10 citations

"Quantitative distribution and local..." refers background or methods in this paper

...The study was performed on sixteen (16) young male Wistar rats, their body weight at the beginning of the experiment within 160-180 g (the mean body weight: 170 ± 10 g)....
[...]
...In addition, it has been shown that reactivity to CGRP significantly enhanced in spontaneously hypertensive rats (16) and that CGRP has a significant role in eosinophilia in allergic inflammation (17)....
[...]
...Apart from one report (16) indicating an increased number and staining intensity of CGRP immunoreactive gastric cells in spontaneous hypertension, no information on the expression of this neuropeptide in the cells of the stomach in the renal hypertension is available....
[...]

Journal Article•DOI•

Interface between the intestinal environment and the nervous system

[...]

Ove Lundgren¹•Institutions (1)

University of Gothenburg¹

01 Mar 2004-Gut

TL;DR: Possible mechanisms by which the intestinal contents may influence afferent nerve fibres in the lamina propria of the intestinal mucosa are discussed, as well as enterocytes, as sensors for luminal microbes.

...read moreread less

Abstract: Possible mechanisms by which the intestinal contents may influence afferent nerve fibres in the lamina propria of the intestinal mucosa are discussed in this brief review. After addressing intestinal epithelial permeability, endocrine and brush cells are discussed, as well as enterocytes, as sensors for luminal microbes.

...read moreread less

10 citations

Journal Article•DOI•

Immunohistochemical and electron-microscopic identification of neuroendocrine cells in the stomach of uremic rats

[...]

Irena Kasacka¹•Institutions (1)

Medical University of Białystok¹

01 Jun 2004-Cell Biology International

TL;DR: The analysis showed an increased number of APUD cells in the stomach of uremic rats compared to control rats, which may be a morphological expression of their hyperfunction in the functional impairment of renal parenchyma, suggesting that chronic renal failure can modulate the secretory processes of AP UD cells.

...read moreread less

6 citations

"Quantitative distribution and local..." refers background in this paper

...The results of numerous studies (19-21) seem to indicate that in various pathological states the number and morphology of JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 2009, 60, 2, 35-39...
[...]

Journal Article•DOI•

Nitric oxide synthase activity in rat gastric mucosa contributes to mucin synthesis elicited by calcitonin gene-related peptide.

[...]

Takafumi Ichikawa¹, Tatsumi Kusakabe², Yukari Gono¹, Nobuaki Shikama¹, Hiromi Hiruma¹, Tadashi Kawakami¹, Kazuhiko Ishihara¹ - Show less +3 more•Institutions (2)

Kitasato University¹, Kokushikan University²

01 Jun 2006-Biomedical Research-tokyo

TL;DR: Findings demonstrate the age-dependent reduction in the C GRP-induced mucin biosynthesis, as well as in the density of the CGRP fibers in the rat stomach, which may be a principal cause for the lack of regulation of the mucIn biosynthesis by CGRp.

...read moreread less

Abstract: The majority of research for the calcitonin gene-related peptide (CGRP) in the stomach has been devoted to the submucosal blood flow, and only slight attention has been paid to its involvement in the gastric epithelial function. In this study, we examined the age-related change in the CGRP-containing nerves and its effects on the mucus metabolism. We compared the immunoreactivity for CGRP in the gastric mucosa of 7-week-old rats (young) to that of 52-week-old animals (middle-aged). The effects of CGRP on the mucin biosynthesis were compared using the stomachs from both young and middle-aged rats. The nitric oxide synthase (NOS) activity was measured in the surface and deep mucosa of the gastric corpus. The density of the CGRP nerve fibers was reduced in both the lamina propria and submucosa of the middle-aged rats compared to the young rats. CGRP stimulated the mucin biosynthesis in the cultured corpus mucosa from the 7-week-old rats, but not from the 52-week-old rats. The total NOS activity of the surface layer in the corpus mucosa was markedly reduced in the middle-aged rats compared to the young rats. These findings demonstrate the age-dependent reduction in the CGRP-induced mucin biosynthesis, as well as in the density of the CGRP fibers in the rat stomach. The decreased NOS activity in the surface layer of the oxyntic mucosa in the aged rats may also be a principal cause for the lack of regulation of the mucin biosynthesis by CGRP.

...read moreread less

5 citations

"Quantitative distribution and local..." refers background in this paper

...CGRP can stimulate somatostatin release and exerts stimulatory effects on the mucin biosynthesis in the surface epithelial cells of the gastric mucosa (27)....
[...]

Journal Article•

Quantitative characteristics of somatostatin-like cells in the stomach of uraemic rats.

[...]

Irena Kasacka¹•Institutions (1)

Medical University of Białystok¹

01 Mar 2006-Journal of Physiology and Pharmacology

TL;DR: The results can be regarded as a morphological manifestation of the hyperreaction of somatostatin-producing endocrine cells in the rat stomach to disorders in the internal environment of the body induced by impairment of renal parenchyma function.

...read moreread less

Abstract: Metabolic disorders induced by impairment of renal parenchyma functions affect the activity of the endocrine cells of the APUD system, which are of importance in the intrinsic regulatory system in the digestive tract. For this reason, the author decided to investigate the behaviour of neuroendocrine cells in experimental uraemia, taking somatostatin-producing cells as an example. The aim of the present study was to examine the number and distribution of somatostatin-containing cells in the pylorus of rats with uraemia. Segments of the gastric pylorus were collected 1, 2 and 4 weeks after nephrectomy. Paraffin-embedded sections were stained with H+E and by silver impregnation. To identify the neuroendocrine cells, on immunohistochemical reaction was performed with a specific antibody against somatostatin. It was found that the number of ST-immunoreactive cells in the stomach of the rats significantly decreased one week after nephrectomy and then considerably increased two and four weeks after the uraemia-inducing surgery as compared with the values in the control animals. The results can be regarded as a morphological manifestation of the hyperreaction of somatostatin-producing endocrine cells in the rat stomach to disorders in the internal environment of the body induced by impairment of renal parenchyma function.

...read moreread less

5 citations

"Quantitative distribution and local..." refers background in this paper

...The results of numerous studies (19-21) seem to indicate that in various pathological states the number and morphology of JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 2009, 60, 2, 35-39...
[...]