Quorum sensing controls biofilm formation in Vibrio cholerae.
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TLDR
The distinct environments occupied by this aquatic pathogen presumably include niches where cell‐cell communication is crucial, as well as ones where loss of quorum sensing via hapR mutation confers a selective advantage.Abstract:
Summary
Multiple quorum-sensing circuits function in parallel to control virulence and biofilm formation in Vibrio cholerae. In contrast to other bacterial pathogens that induce virulence factor production and/or biofilm formation at high cell density in the presence of quorum-sensing autoinducers, V. cholerae represses these behaviours at high cell density. Consistent with this, we show here that V. cholerae strains ‘locked’ in the regulatory state mimicking low cell density are enhanced for biofilm production whereas mutants ‘locked’ in the regulatory state mimicking high cell density are incapable of producing biofilms. The quorum-sensing cascade we have identified in V. cholerae regulates the transcription of genes involved in exopolysaccharide production (EPS), and variants that produce EPS and form biofilms arise at high frequency from non-EPS, non-biofilm producing strains. Our data show that spontaneous mutation of the transcriptional regulator hapR is responsible for this effect. Several toxigenic strains of V. cholerae possess a naturally occurring frameshift mutation in hapR. Thus, the distinct environments occupied by this aquatic pathogen presumably include niches where cell-cell communication is crucial, as well as ones where loss of quorum sensing via hapR mutation confers a selective advantage. Bacterial biofilms could represent a complex habitat where such differentiation occurs.read more
Citations
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QUORUM SENSING: Cell-to-Cell Communication in Bacteria
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Biofilms: the matrix revisited
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Bacterial Quorum Sensing: Its Role in Virulence and Possibilities for Its Control
TL;DR: This work reviews the quorum-sensing circuits of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, and Vibrio cholerae and examines recent efforts to inhibit quorum sensing in these pathogens with the goal of designing novel antimicrobial therapeutics.
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Bacterial Quorum-Sensing Network Architectures
Wai Leung Ng,Bonnie L. Bassler +1 more
TL;DR: It is argued that the Vibrio quorum-sensing systems are optimally designed to precisely translate extracellular autoinducer information into internal changes in gene expression.
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Biodiversity of Vibrios
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