Rad51 Protein Controls Rad52-mediated DNA Annealing
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Cites background from "Rad51 Protein Controls Rad52-mediat..."
...For example, Rad51 prevents Rad52-mediated annealing of complementary ssDNA in yeast [91,92], and RAD51 inhibition in mammalian cells upregulates RAD52-mediated SSA activity [17]....
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Cites background from "Rad51 Protein Controls Rad52-mediat..."
...BLM may also contribute to a noncrossover outcome by promoting the SDSA pathway, as indicated by genetic studies in Drosophila (3)....
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...SDSA, which does not have these deleterious consequences, is the favored subpathway (123)....
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...In the presence of a second end, the predominant pathway for DSB repair in somatic cells appears to be synthesis-dependent strand annealing (SDSA), in which the extended D-loop is reversed, leading to annealing of the newly synthesized strand with the resected strand of the second end (Figure 1) (123)....
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...The DNA damage sensitivity profile of RTEL1 mutants is more consistent with a defect in HR, suggesting that RTEL1 may play a role in SDSA (169)....
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...F or p er so na l u se o nl y. Displacement loop (D-loop): primary DNA-strand invasion product of the Rad51-ssDNA filament leading to the different HR subpathways (BIR, SDSA, dHJ) Break-induced replication (BIR): a subpathway of HR where a single-ended DSB invades and establishes a fullfledged replication fork Holliday junction (HJ): Cross-stranded joint molecule HR intermediate Synthesis-dependent strand annealing (SDSA): a subpathway of HR where the second end of the DSB anneals with the extended strand of the first end Double Holliday junction (dHJ): HR intermediate leading to crossovers....
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763 citations
Cites background from "Rad51 Protein Controls Rad52-mediat..."
...We suggest that annealing is catalyzed by Rad52....
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...Rad52 is essential for the single-strand annealing reaction that PLoS Genetics | www.plosgenetics.org 6 January 2009 | Volume 5 | Issue 1 | e1000327 deletes sequence between direct repeats [96], and it anneals single strands in vitro [97]....
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...These observations show that microhomology junction formation can be mediated by a different protein in yeast, as well as by Rad52....
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...In one of these cases, frequent switches were associated with microhomology junctions in a Rad51-independent, Rad52-dependent process that produced translocations and inversions at sites of highly diverged genes [66]....
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...In vitro, Rad51 inhibits the single-strand annealing activity of Rad52 [99], suggesting that the absence of Rad51 might exercise tight control of the switch from strand invasion to annealing of single strands....
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References
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