Journal ArticleDOI
Rapid analysis of amino acids using pre-column derivatization.
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TLDR
The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydrolyzates and the liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids.About:
This article is published in Journal of Chromatography A.The article was published on 1984-12-07. It has received 2397 citations till now. The article focuses on the topics: Derivatization & Amino acid.read more
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Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.
James S. Huston,D. Levinson,Meredith Mudgett-Hunter,M S Tai,Jirí Novotný,Michael N. Margolies,R J Ridge,Robert E. Bruccoleri,Edgar Haber,R Crea +9 more
TL;DR: A biosynthetic antibody binding site, which incorporated the variable domains of anti-digoxin monoclonal antibody 26-10 in a single polypeptide chain, was produced in Escherichia coli by protein engineering.
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Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation.
Terrence L. Geiger,Steven Clarke +1 more
TL;DR: These studies indicate that both aspartic acid and asparagine residues may be hot spots for the nonenzymatic degradation of proteins, especially in cells such as erythrocytes and eye lens, where these macromolecules must function for periods of about 120 days and 80 years, respectively.
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Nutritional properties of microalgae for mariculture
TL;DR: This work has studied the biochemical composition of about 40 species of microalgae from seven algal classes to define those that may be best adapted to the Australian conditions.
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A Polypeptide from Tomato Leaves Induces Wound-Inducible Proteinase Inhibitor Proteins
TL;DR: It is established that a polypeptide factor can initiate signal transduction to regulate the synthesis of defensive proteins in plant tissues.
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Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells
James W. Fett,Daniel J. Strydom,Roy R. Lobb,Edward M. Alderman,J. Lemuel Bethune,James F. Riordan,Bert L. Vallee +6 more
TL;DR: The first human tumor derived protein with in vivo angiogenic activity to be obtained in pure form has been isolated from serum-free supernatants of an established human adenocarcinoma cell line (HT-29) and named angiogenin.
References
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Journal ArticleDOI
Chromatography of Amino Acids on Sulfonated Polystyrene Resins. An Improved System
TL;DR: A systematic study has been made of the separations of amino acids by elution analysis on columns of synthetic ion exchange resins, demonstrating that synthetic resins are capable of separating most of the common amino acids from one another.
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O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin
James R. Benson,P. E. Hare +1 more
TL;DR: O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products that can be detected easily and soluble and stable in aqueous buffers.
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Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits.
TL;DR: Peptide mapping by sodium dodecyl sulfate-polyacrylamide gel electrophoresis following treatment with papain, chymotrypsin, or Staphylococcus aureus V8 protease and by high performance liquid chromatography following trypsinolysis indicates that form 3a is a unique gene product.