Rapid and accurate detection of SARS-CoV-2 mutations using a Cas12a-based sensing platform
He Changsheng,Cailing Lin,Guosheng Mo,Binbin Xi,An′an Li,Dongchao Huang,Yanbin Wan,Feng Chen,Yufeng Liang,Qingxia Zuo,Wanqing Xu,Dongyan Feng,Guanting Zhang,Liya Han,Changwen Ke,Hongli Du,Lizhen Huang +16 more
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TLDR
In this paper, a Cas12a-based RT-PCR combined with CRISPR on-site rapid detection system (RT-CORDS) platform was used to detect the key mutations in SARS-CoV-2 variants, such as 69/70 deletion, N501Y, and D614G.About:
This article is published in Biosensors and Bioelectronics.The article was published on 2021-10-25 and is currently open access. It has received 21 citations till now. The article focuses on the topics: Trans-activating crRNA & CRISPR.read more
Citations
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Long-Lasting Chemiluminescence-Based POCT for Portable and Visual Pathogenic Detection and In Situ Inactivation.
TL;DR: Bacterial infections can be eliminated with high efficiency due to excellent photothermal property of CuSeNPs@MPBA, and the developed multifunctional platform has advantages of simple operation with low cost, suggesting its high potential for use in food safety, environment monitoring, and clinical applications.
Journal ArticleDOI
REVEALR-Based Genotyping of SARS-CoV-2 Variants of Concern in Clinical Samples
TL;DR: REVEALR is designed into a novel genotyping assay that detects single-base mismatches corresponding to each of the major SARS-CoV-2 strains found in the United States, and offers an important new approach to personalized diagnostics.
Journal ArticleDOI
An Update on Detection Technologies for SARS-CoV-2 Variants of Concern
TL;DR: A range of research has been reported on detection techniques for VOCs, which is beneficial to prevent the rapid spread of the epidemic, improve the effectiveness of public health and social measures, and reduce the harm to human health and safety as discussed by the authors .
Journal ArticleDOI
Emerging Multiplex Nucleic Acid Diagnostic Tests for Combating COVID-19
TL;DR: A comprehensive and up-to-date overview of multiplex assays that incorporate various detection reactions and readout modalities can be found in this paper , followed by highlights of platforms that are amenable for point-of-care use.
Posted ContentDOI
A multiplexed Cas13-based assay with point-of-care attributes for simultaneous COVID-19 diagnosis and variant surveillance
Maturada Patchsung,Aimorn Homchan,Kanokpol Aphicho,Surased Suraritdechachai,T Wanitchanon,Archiraya Pattama,Khomkrit Sappakhaw,Piyachat Meesawat,Thanakrit Wongsatit,A. Athipanyasilp,Krittapas Jantarug,Niracha Athipanyasilp,Juthamas Buahom,S. Visanpattanasin,Nootaree Niljianskul,Pimchai Chaiyen,Ruchanok Tinikul,Nuanjun Wichukchinda,Surakameth Mahasirimongkol,Rujipas Sirijatuphat,Nasikarn Angkasekwinai,Michael A. Crone,Paul S. Freemont,J. Keith Joung,Amit Ladha,Omar Abudayyeh,Jonathan S. Gootenberg,Claire Chewapreecha,Sittinan Chanarat,Navin Horthongkham,Danaya Pakotiprapha,Chayasith Uttamapinant +31 more
TL;DR: A robust multiplexed CRISPR-based detection using LwaCas13a and PsmCas13b to simultaneously diagnose SARS-CoV-2 infection and pinpoint the causative Sars-Cov-2 variant of concern (VOC)-including globally dominant VOCs Delta, Omicron, and B.1.617.
References
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Journal ArticleDOI
Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus.
Bette T. Korber,Will Fischer,Sandrasegaram Gnanakaran,Hyejin Yoon,James Theiler,Werner Abfalterer,Nick Hengartner,Elena E. Giorgi,Tanmoy Bhattacharya,Brian T. Foley,Kathryn M. Hastie,Matthew Parker,David G Partridge,Cariad Evans,Timothy M. Freeman,Thushan I de Silva,Adrienne Angyal,Rebecca Brown,Laura Carrilero,Luke R. Green,Luke R. Green,Luke R. Green,Danielle C. Groves,Katie Johnson,Alexander J Keeley,Benjamin B Lindsey,Paul J. Parsons,Mohammad Raza,Sarah Rowland-Jones,Nikki Smith,Rachel Tucker,Dennis Wang,Matthew Wyles,Charlene McDanal,Lautaro G. Perez,Haili Tang,Alex Moon-Walker,Alex Moon-Walker,Alex Moon-Walker,Sean P. J. Whelan,Celia C. LaBranche,Erica Ollmann Saphire,David C. Montefiori +42 more
TL;DR: A SARS-CoV-2 variant carrying the Spike protein amino acid change D614G has become the most prevalent form in the global pandemic, and it is found that the G614 variant grows to higher titer as pseudotyped virions.
Journal ArticleDOI
SARS-CoV-2 variants, spike mutations and immune escape.
William T. Harvey,Alessandro M Carabelli,Ben Jackson,Ravindra K. Gupta,E. Thomson,E. Thomson,Ewan M. Harrison,Ewan M. Harrison,Catherine Ludden,Richard Reeve,Andrew Rambaut,Sharon J. Peacock,David Robertson +12 more
TL;DR: A review of the literature on mutations of the SARS-CoV-2 spike protein, the primary antigen, focusing on their impacts on antigenicity and contextualizing them in the protein structure is presented in this article.
Journal ArticleDOI
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Janice S. Chen,Enbo Ma,Lucas B. Harrington,Maria Da Costa,Xinran Tian,Joel M. Palefsky,Jennifer A. Doudna +6 more
TL;DR: It is shown that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA cleavage activity by Cas12a that completely degrades ssDNA molecules, which is also a property of other type V CRISPR-Cas12 enzymes.
Journal ArticleDOI
Estimated transmissibility and impact of SARS-CoV-2 lineage B.1.1.7 in England.
Nicholas G Davies,Sam Abbott,Rosanna C. Barnard,Christopher I Jarvis,Adam J. Kucharski,James D Munday,Carl A. B. Pearson,Timothy W Russell,Damien C. Tully,Alex D. Washburne,Tom Wenseleers,Amy Gimma,William Waites,Kerry L. M. Wong,Kevin van Zandvoort,Justin D. Silverman,Karla Diaz-Ordaz,Ruth H. Keogh,Rosalind M Eggo,Sebastian Funk,Mark Jit,Katherine E. Atkins,Katherine E. Atkins,W. John Edmunds +23 more
TL;DR: Using a variety of statistical and dynamic modeling approaches, the authors estimate that this variant has a 43 to 90% (range of 95% credible intervals, 38 to 130%) higher reproduction number than preexisting variants, and a fitted two-strain dynamic transmission model shows that VOC 202012/01 will lead to large resurgences of COVID-19 cases.
Journal ArticleDOI
CRISPR-Cas12-based detection of SARS-CoV-2.
James Paul Broughton,Xianding Deng,Guixia Yu,Clare L Fasching,Venice Servellita,Jasmeet Singh,Xin Miao,Jessica Streithorst,Andrea Granados,Alicia Sotomayor-Gonzalez,Kelsey C. Zorn,Allan Gopez,Elaine Hsu,Wei Gu,Steve Miller,Chao Yang Pan,Hugo Guevara,Debra A. Wadford,Janice S. Chen,Charles Y. Chiu +19 more
TL;DR: The CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT–PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.