Journal ArticleDOI
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
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TLDR
Extension of this method to concentration and purification of other viruses and nucleic acids, as well as some preliminary mechanistic studies, are discussed.About:
This article is published in Virology.The article was published on 1970-03-01. It has received 1454 citations till now. The article focuses on the topics: Polyethylene glycol & PEG ratio.read more
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Analysis of bacteriophage T7 early RNAs and proteins on slab gels
TL;DR: The RNAs and proteins specified by five early genes of bacteriophage T7 have been identified by electrophoresis on sodium dodecyl sulfate, polyacrylamide gels using a slab gel system in which 25 samples can be run simultaneously and then dried for autoradiography.
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Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
TL;DR: The LiAc/SS‐DNA/PEG method was shown to be more effective than other treatments known to make cells transformable and caused tighter binding of 32P‐labelled plasmid DNA than did double‐stranded (DS) carrier.
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Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials
TL;DR: Strategies for exosome isolation, the understanding to date of exosomes composition, functions, and pathways, and their potential for diagnostic and therapeutic applications are summarized.
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Lambda replacement vectors carrying polylinker sequences
TL;DR: A new family of lambda replacement vectors and derivatives containing amber mutations and derivatives of the EMBL3 vector allow the application of genetic screening procedures based on selection for the products of homologous recombination events, and for the selective cloning of DNA sequences linked to supF genes.
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The genetics and physiology of bacteriophage T7
TL;DR: It appears that in T7 (as in T4 and lambda) related functions tend to cluster along the genetic map, which makes this system a very attractive one in which to study a number of biological problems at the molecular level.