Rapid Diffusion of Green Fluorescent Protein in the Mitochondrial Matrix
Citations
225 citations
Cites background from "Rapid Diffusion of Green Fluorescen..."
...Instead, Partikian et al. (1998) suggested that the arrangement of metabolic pathways into metabolons, particles containing the enzymes of a part or the whole of a metabolic pathway (Robinson and Srere, 1985; Velot et al., 1997), enabled the establishment of an uncrowded, enzyme-free, aqueous space…...
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...…to the matrix of mitochondria in various types of animal cell is fully dispersed throughout the available space and FRAP (fluorescence recovery after photobleaching) studies have shown diffusion rates of GFP to be close to that of a protein in a dilute aqueous solution (Partikian et al., 1998)....
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212 citations
Cites background from "Rapid Diffusion of Green Fluorescen..."
...The mean values of 19–20 ns were consistent with other measurements of GFPs (including EGFP) in solution (Partikian et al., 1998; Volkmer et al., 2000; Heikal et al., 2001)....
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...For example, certain dyes used as microviscosity probes can give misleading results if they bind to (but not partition into) the cellular compartment of interest (Partikian et al., 1998)....
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206 citations
Cites methods from "Rapid Diffusion of Green Fluorescen..."
...These techniques have been used to measure the diffusion of GFP and GFP fusion proteins within animal mitochondria (Partikian et al., 1998) and within a single E.coli cell (Elowitz et al., 1999), and therefore should be applicable to plant plastids, mitochondria , and nuclei....
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202 citations
191 citations
Additional excerpts
...Values of r0 (1) reported by Partikian et al. (1998) and in this work approach the limiting value of 0.4, supporting the assumption that 0 for GFPs....
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...The measured OPE r0 agreed well with the value of 0.39 for humanized red-shifted GFP recently reported by Partikian et al. (1998)....
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References
2,594 citations
"Rapid Diffusion of Green Fluorescen..." refers background in this paper
...As discussed by Axelrod et al. (1976) for conventional two-dimensional spot photobleaching, this approximation is reasonably valid for practical laser/lens systems; the same considerations would apply for bleaching of long thin mitochondria where bleach profile is nearly constant across the thin…...
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2,232 citations
1,502 citations
"Rapid Diffusion of Green Fluorescen..." refers background in this paper
...The three–amino acid chromophore in GFP is fixed rigidly within a barrel structure (Yang et al., 1996; Örmo et al., 1996)....
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1,017 citations