Journal ArticleDOI
Rapid extraction of bacterial genomic DNA with guanidium thiocyanate
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TLDR
The method, which was applicable to both Gram‐positive and Gram‐negative bacteria, eliminated endogenous nuclease activity and avoided the need for phenol, RNase and protease treatments.Abstract:
A method is described for the rapid isolation and purification of bacterial genomic DNA. A total of 215 bacterial strains representing species of Campylobacter, Corynebacterium, Escherichia, Legionella, Neisseria, Staphylococcus and Streptococcus, were lysed with guanidium thiocyanate. DNA was prepared using just three other reagents and one high-speed centrifugation step. The method, which was applicable to both Gram-positive and Gram-negative bacteria, eliminated endogenous nuclease activity and avoided the need for phenol, RNase and protease treatments. The DNA was of high purity, high molecular mass and double-stranded.read more
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Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
George A. O'Toole,Roberto Kolter +1 more
TL;DR: The isolation and characterization of mutants of Pseudomonas aeruginosa PA14 defective in the initiation of biofilm formation on an abiotic surface, polyvinylchloride (PVC) plastic are reported and evidence that microcolonies form by aggregation of cells present in the monolayer is presented.
Journal ArticleDOI
Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis
George A. O'Toole,Roberto Kolter +1 more
TL;DR: The genetic analyses suggest that biofilm formation can proceed via multiple, convergent signalling pathways, which are regulated by various environmental signals, and that of the 24 sad mutants analysed in this study, only three had defects in genes of known function.
Journal ArticleDOI
A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis
Kathleen Machiels,Marie Joossens,João Sabino,Vicky De Preter,Ingrid Arijs,Venessa Eeckhaut,Vera Ballet,Karolien Claes,Filip Van Immerseel,Kristin Verbeke,Marc Ferrante,Jan Verhaegen,Paul Rutgeerts,Severine Vermeire +13 more
TL;DR: The composition of the fecal microbiota of UC patients differs from that of healthy individuals: the authors found a reduction in R hominis and F prausnitzii, both well-known butyrate-producing bacteria of the Firmicutes phylum, suggesting that different bacterial species contribute to the pathogenesis of UC and CD.
Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Secretion of Flavins by Shewanella Species and Their Role in Extracellular Electron Transfer
TL;DR: Flavin mononucleotide and riboflavin are identified for the first time as the extracellular electron shuttles produced by a range of Shewanella species and shown to act as electron Shuttles and to promote anoxic growth coupled to the accelerated reduction of poorly crystalline Fe(III) oxides.
References
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Journal ArticleDOI
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
Determination of DNA base composition by reversed-phase high-performance liquid chromatography
Jin Tamaoka,Kazuo Komagata +1 more
TL;DR: The system described here gives a direct and precise method for determining DNA base composition by reversed-phase high-performance liquid chromatography (HPLC).
Journal ArticleDOI
Rapid preparation of DNA from filamentous fungi
U. Raeder,P. Broda +1 more
TL;DR: A general, simple and inexpensive method for the isolation of DNA from filamentous fungi, starting from freeze‐dried mycelium 01–015% by weight, which allows the processing of many samples in parallel.
Journal ArticleDOI
A small-scale five-hour procedure for isolating multiple samples of CsCl-purified DNA: Application to isolations from mammalian, insect, higher plant, algal, yeast, and bacterial sources
TL;DR: A rapid and simple procedure is described for obtaining CsCl-purified DNA from multiple small samples of cells or tissue and can be scaled up with larger sample sizes and longer centrifugation times to provide bulk quantities of DNA.