Rapid method for determining alkaline phosphatase activity in serum with thymolphthalein monophosphate.
About: This article is published in Clinical Chemistry.The article was published on 1970-05-01. It has received 152 citations till now. The article focuses on the topics: Alkaline phosphatase & Colorimetry (chemical method).
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01 Sep 1973
TL;DR: The CRC Critical Reviews in Clinical Laboratory Sciences: Vol. 4, No. 3, No 3, pp 215-340 as discussed by the authors was the first publication of a comprehensive review of laboratory tests for the assessment of nutritional status.
Abstract: (1973). Laboratory Tests for the Assessment of Nutritional Status. CRC Critical Reviews in Clinical Laboratory Sciences: Vol. 4, No. 3, pp. 215-340.
458 citations
TL;DR: In addition to the bone source, isoenzymes of alkaline phosphatase from liver, intestine and placenta have now been found to contribute importantly to the serum, either individually, or in combination, which provides new insights into the mechanism of uncompetitive inhibition.
400 citations
TL;DR: In all dosage groups the most marked relief of pain was at sites of Paget's involvement of long bones, and there was a dose-response relation of administered disodium etidronate to improvement of pain, with moderate or marked improvement ofPain in six of seven subjects on 20 mg per kilogram per day.
Abstract: A six-month double-blind controlled study was performed to determine effectiveness and dose response of disodium etidronate on Paget's disease (osteitis deformans). Forty-seven subjects were studied with pain related to radiologically documented Paget's bone sites as well as marked elevation of serum alkaline phosphatase and hydroxyproline excretion. There was significant (p<0.05) reduction of alkaline phosphatase, hydroxyproline and radiofluoride or radiostrontium uptake over Paget's bone lesions after orally administered disodium etidronate at doses of 5, 10 and 20 mg per kilogram per day for six months. There was a dose-response relation of administered disodium etidronate to improvement of pain, with moderate or marked improvement of pain in six of seven subjects on 20 mg per kilogram per day. In all dosage groups the most marked relief of pain was at sites of Paget's involvement of long bones. Apparent side effects were limited to occasional abdominal cramps. (N Engl J Med 289:1379–1384, 1973)
239 citations
TL;DR: Benefits of the use of sodium thymolphthalein monophosphate as a substrate for measuring acid phosphatase activity in serum include simplicity, good sensitivity over a wide range of activity, high precision, zero-order kinetics to about 200 times the upper limit of normal, easy standardization by a pure standard, and reagent stability.
Abstract: We describe the use of sodium thymolphthalein monophosphate as a substrate for measuring acid phosphatase activity in serum. Thymolphthalein is liberated in the reaction and is conveniently measured by increasing the pH of the medium, which produces a color and also stops the hydrolysis. Optimal conditions for enzymatic activity were defined for use in the final assay system. Purified acid phosphatase isoenzymes from various human tissues and sera from patients with nonprostatic diseases most likely to affect serum acid phosphatase activity were used to show that the new method is more specific for the prostatic enzyme than other commonly used procedures for acid phosphatase. Other advantages include simplicity, good sensitivity over a wide range of activity, high precision, zero-order kinetics to about 200 times the upper limit of normal, easy standardization by a pure standard, and reagent stability.
229 citations
TL;DR: Investigation of the ability of these volatile oils to scavenge free radicals generated during aflatoxicosis found Nigella sativa oil was found to be more effective than Syzygium aromaticum oil in restoring the parameters that were altered by aflatoxin in rats.
Abstract: Aflatoxins, a group of closely related, extremely toxic mycotoxins produced by Aspergillus flavus and A. parasiticus, can occur as natural contaminants of foods and feeds. Aflatoxins have been shown to be hepatotoxic, carcinogenic, mutagenic and teratogenic to different animal species. Nigella sativa (black cumin) and Syzygium aromaticum (clove) oil are used for the treatment of inflammatory diseases and have antioxidant properties. The aim of this study was to investigate the ability of these volatile oils to scavenge free radicals generated during aflatoxicosis. Sixty male rats were divided into six treatment groups, including a control group, and the groups were treated for 30 days with Nigella sativa and Syzygium aromaticum oils with or without aflatoxin. Blood samples were collected at the end of the experimental period for haematological and biochemical analysis. The results indicated that exposure to aflatoxins resulted in haematological and biochemical changes typical for aflatoxicosis. Treatment with Nigella sativa and Syzygium aromaticum oil of rats fed an aflatoxin-contaminated diet resulted in significant protection against aflatoxicosis. Moreover, Nigella sativa oil was found to be more effective than Syzygium aromaticum oil in restoring the parameters that were altered by aflatoxin in rats.
212 citations