Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
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...The stress granules were imaged using a fast DSLM microscope with structured illumination (Planchon et al., 2011)....
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1,585 citations
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...S4) conceptually similar to ourearlierBesselmicroscope (7,8)....
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...In response, we introduced (7) “nondiffracting”Bessel beams to light sheetmicroscopy (Fig....
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...Specimens are cultured or mounted on 5-mmdiameter cover slips (Warner Instruments, 640700) cleaned before use according to our earlier protocol (7)....
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...Certain aspects of the design are derived from our earlier Bessel beam work, so these earlier publications (7, 8, 57) remain valuable resources as well....
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...This is about an order of magnitude more images per specimen than we achieved with linear Bessel beam plane illumination (7, 8) and nearly two orders of magnitude more than in typical confocal data sets of similar specimens at similar expression levels....
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902 citations
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...…illumination microscopy (Holekamp et al., 2008), diSPIM (Wu et al., 2013), two-photon lightsheet (Planchon et al., 2011), Bessel beam lightsheet (Planchon et al., 2011), lattice lightsheet (Chen et al., 2014), and SCAPE (Bouchard et al., 2015), are limited to samples only a few hundreds…...
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...…axial resolution, including objectivecoupled planar illumination microscopy (Holekamp et al., 2008), diSPIM (Wu et al., 2013), two-photon lightsheet (Planchon et al., 2011), Bessel beam lightsheet (Planchon et al., 2011), lattice lightsheet (Chen et al., 2014), and SCAPE (Bouchard et al., 2015),…...
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..., 2011), Bessel beam lightsheet (Planchon et al., 2011), lattice lightsheet (Chen et al....
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..., 2013), two-photon lightsheet (Planchon et al., 2011), Bessel beam lightsheet (Planchon et al....
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