Rapid uncoating of vector genomes is the key to efficient liver transduction with pseudotyped adeno-associated virus vectors.
Citations
1,930 citations
1,209 citations
Additional excerpts
...5 μg of pTRCMVLuc and pXR (1-9) and 15 μg of pXX680....
[...]
802 citations
792 citations
669 citations
References
1,573 citations
"Rapid uncoating of vector genomes i..." refers background or result in this paper
...Vectors based on AAV2 are being tested in a clinical trial of liver-directed gene transfer for hemophilia B, but recent studies suggest that vector pseudotypes composed of AAV2 VG packaged within AAV6 or AAV8 capsids are more efficient than AAV2 vectors at transducing the liver (9, 11)....
[...]
...As seen in this and a previous study (9), AAV8 capsids packaging ss VG are also able to overcome the 10% limit....
[...]
...It has become apparent that enhanced transduction of refractive tissues can be effected if the AAV2 vector genome (VG) is packaged inside capsids from alternative AAV2 serotypes, of which at least eight have been isolated to date (5, 9, 11, 27)....
[...]
812 citations
"Rapid uncoating of vector genomes i..." refers background in this paper
...Fluorescently labeled AAV capsids have also been detected inside the nucleus after virus infection of cultured cells (28, 30)....
[...]
808 citations
"Rapid uncoating of vector genomes i..." refers background in this paper
...It has become apparent that enhanced transduction of refractive tissues can be effected if the AAV2 vector genome (VG) is packaged inside capsids from alternative AAV2 serotypes, of which at least eight have been isolated to date (5, 9, 11, 27)....
[...]
796 citations
"Rapid uncoating of vector genomes i..." refers background in this paper
...(16) reported that an sc AAV vector expressing erythropoietin (EPO) resulted in more rapid elevation of hematocrit than an ss vector....
[...]
...sc AAV2 vectors have been reported to mediate much more efficient transduction than ss AAV2 vectors in vitro and in vivo (16)....
[...]
786 citations