Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation
Citations
2,866 citations
1,500 citations
1,256 citations
Cites background or methods from "Rational design of highly active sg..."
...[8] was used as rank-percent values as provided; we also converted parts...
[...]
...[8, 34] are not shown here but gave an almost identical correlation profile....
[...]
...A relationship between GC content and specificity is known from siRNA design [30] and would explain the previously observed difficulty to target GC-rich genes [2, 8, 31] and quadruplex-forming sequences [32]....
[...]
...The guide sequence also determines the efficiency of ontarget cleavage [7, 8]; thus, current genome editing protocols recommend [9] that researchers select guides carefully to minimize potential off-target effects and test several to optimize on-target activity....
[...]
1,239 citations
1,142 citations
References
34,830 citations
12,865 citations
12,265 citations
10,746 citations
8,197 citations