Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence.
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3,332 citations
Cites background from "Rb-mediated heterochromatin formati..."
...This may imply that, once senescence is established, unknown mechanisms—potentially related to chromatin alterations—permanently lock the SASP in an irreversible open chromatin confirmation, analogous to the way the p16INK4a/pRB pathway is proposed to lock growth-promoting genes into a heterochromatic state (103)....
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2,114 citations
Cites background from "Rb-mediated heterochromatin formati..."
...Replicatively senescent cells develop dense foci of heterochromatin (Narita et al., 2003; Zhang et al., 2005) which coincide with pRBdependent heterochromatic repression of genes encoding cyclins and other positive cell cycle regulators (Bandyopadhyay et al., 2002; Narita et al., 2003)....
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...Replicatively senescent cells develop dense foci of heterochromatin (Narita et al., 2003; Zhang et al., 2005) which coincide with pRBdependent heterochromatic repression of genes encoding cyclins and other positive cell cycle regulators (Bandyopadhyay et al....
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...Many of these repressed genes are activation targets of E2F transcription factors (Narita et al., 2003), some of which are converted to transcriptional repressors when complexed with pRB (Trimarchi and Lees, 2002)....
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...…mechanisms are as yet unknown, the senescence response appears to result in a reorganization of chromatin, at least some aspects of which require pRB activity (Jacobs et al., 1999; Leung et al., 2001; Bandyopadhyay et al., 2002; Itahana et al., 2003; Narita et al., 2003; Zhang et al., 2003, 2005)....
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..., 2005) which coincide with pRBdependent heterochromatic repression of genes encoding cyclins and other positive cell cycle regulators (Bandyopadhyay et al., 2002; Narita et al., 2003)....
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2,074 citations
2,074 citations
Cites background from "Rb-mediated heterochromatin formati..."
...Some senescent cells contain senescence-associated heterochromatin foci (SAHF): cytologically detectable heterochromatin domains that also contain (and presumably silence) certain proproliferative genes (60)....
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...For example, global chromatin relaxation (such as that caused by broad-acting histone deacetylase inhibitors) induces senescence, often by derepressing the p16INK4a tumor suppressor (61), which promotes the formation of senescence-associated heterochromatin (60)....
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...Cellular senescence entails widespread changes in chromatin organization (59), including the formation of repressive heterochromatin at several loci that encode proproliferative genes (60)....
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References
6,696 citations
"Rb-mediated heterochromatin formati..." refers background in this paper
...(SA- -gal) activity (Campisi, 2001; Dimri et al., 1995; In many instances, p53 and Rb are activated to proShelton et al., 1999)....
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5,562 citations
Additional excerpts
...Nevertheless, the Rb family con(Hayflick, 1965)....
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4,770 citations
"Rb-mediated heterochromatin formati..." refers background or methods in this paper
...Allis), HP1 and infections were performed as described (Serrano et al., 1997) (Chemicon), Rb (C-15, Santa Cruz), p107 (C-18, Santa Cruz), and except that amphotropic viruses were used to produce cells for the p130 (C-20, Santa Cruz) antibodies....
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...…5B, Western BlottingAd-GFP was used at an MOI of 4 PFU cell 1 and Ad-E2F1 was used Western blotting analysis was carried out on 20 g whole-cell lysatein a range of 0.03 and 15 PFU cell 1. by using enhanced chemiluminescence (ECL; Amersham) detection as previously described (Serrano et al., 1997)....
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...…cells (G. Nolan, Stanford University, CA) H3 (ab7312, Abcam, Upstate, or provided by C.D. Allis), HP1 and infections were performed as described (Serrano et al., 1997) (Chemicon), Rb (C-15, Santa Cruz), p107 (C-18, Santa Cruz), and except that amphotropic viruses were used to produce cells for…...
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...Recent studies suggest of senescence-associated DNA foci, we focused on the effects of oncogenic ras, as it acutely and reproducibly that p107 and p130, but not Rb, are associated with E2Fresponsive genes during the cell cycle and quiescence induces senescence in IMR90 cells over several days (Serrano et al., 1997)....
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...…MEK1 Q56P) (Lin et al., 1998); pWZL Cells were permeabilized with 0.01% L- -lysophosphatidylcholine Hygro (H-RasV12, p16INK4a, and human p53175H) (Serrano et al., 1997); (Sigma) in 150 mM sucrose, 80 mM KCl, 35 mM HEPES [pH 7.4], 5 pLPC-Puro (EGFP-tagged human HP1 cDNA and E1A and E1A mM K2HPO4,…...
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2,820 citations
"Rb-mediated heterochromatin formati..." refers background in this paper
...Scale bar is equal to 5 m. nister et al., 2001; Lachner et al., 2001), a family of adaptor molecules that are required for heterochromatin assembly and are involved in epigenetic gene regulation....
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2,811 citations
"Rb-mediated heterochromatin formati..." refers background in this paper
...We next examined the occupancy of E2F target proE2F-Responsive Genes Are Stably Repressed moters by K9M-H3 and HP1 : two proteins that are in Senescent Cells enriched in the SAHFs and are known to be involved in In principle, the altered chromatin state accompanying heterochromatin formation (Bannister et al., 2001; SAHF formation and the binding of K9M-H3, HP1, and Lachner et al., 2001)....
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...…by K9M-H3 and HP1 : two proteins that are in Senescent Cellsenriched in the SAHFs and are known to be involved in In principle, the altered chromatin state accompanyingheterochromatin formation (Bannister et al., 2001; SAHF formation and the binding of K9M-H3, HP1, andLachner et al., 2001)....
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